31 research outputs found
Identification of an expanded set of translationally active methionine analogues in Escherichia coli
Amino acid incorporation into proteins in vivo is controlled most stringently by the aminoacyl-tRNA synthetases. Here we report the incorporation of several new methionine analogues into protein by increasing the rate of their activation by the methionyl-tRNA synthetase (MetRS) of Escherichia coli. cis-Crotylglycine (4), 2-aminoheptanoic acid (7), norvaline (8), 2-butynylglycine (11), and allylglycine (12) will each support protein synthesis in methionine-depleted cultures of E. coli when MetRS is overexpressed and the medium is supplemented with the analogue at millimolar concentrations. These investigations suggest important opportunities for protein engineering, as expansion of the translational apparatus toward other amino acid analogues by similar strategies should also be possible
Corrigendum to: Identification of an expanded set of translationally active methionine analogues in Escherichia coli
Design and Synthesis of a Two Compartment Micellar System Based on the Self-Association Behavior of Poly(N-acylethylene imine) End-capped with a Fluorocarbon and a Hydrocarbon Chain
Cylindrical micelles of alpha-fluorocarbon-omega-hydrocarbon end-capped poly(N-acylethylene imine)s
Fine-tuning the transition temperature of a stimuli-responsive polymer by a simple blending procedure
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Reversible Immobilization onto PEG-based Emulsion-templated Porous Polymers by Co-assembly of Stimuli Responsive Polymers
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Synthesis and Characterization of Cationic Hydrogels from Thiolated Copolymers for Independent Manipulation of Mechanical and Chemical Properties of Cell Substrates
Elastin-based side-chain polymers: Improved synthesis via RAFT and stimulus responsive behavior
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