Deletion of Nhlh2 Results in a Defective Torpor Response and Reduced Beta Adrenergic Receptor Expression in Adipose Tissue

Mice with a targeted deletion of the basic helix-loop-helix transcription factor, Nescient Helix-Loop-Helix 2 (Nhlh2), display adult-onset obesity with significant increases in their fat depots, abnormal responses to cold exposure, and reduced spontaneous physical activity levels. These phenotypes, accompanied by the hypothalamic expression of Nhlh2, make the Nhlh2 knockout (N2KO) mouse a useful model to study the role of central nervous system (CNS) control on peripheral tissue such as adipose tissue.Differences in body temperature and serum analysis of leptin were performed in fasted and ad lib fed wild-type (WT) and N2KO mice. Histological analysis of white (WAT) and brown adipose tissue (BAT) was performed. Gene and protein level expression of inflammatory and metabolic markers were compared between the two genotypes.We report significant differences in serum leptin levels and body temperature in N2KO mice compared with WT mice exposed to a 24-hour fast, suggestive of a defect in both white (WAT) and brown adipose tissue (BAT) function. As compared to WT mice, N2KO mice showed increased serum IL-6 protein and WAT IL-6 mRNA levels. This was accompanied by slight elevations of mRNA for several macrophage markers, including expression of macrophage specific protein F4/80 in adipose, suggestive of macrophage infiltration of WAT in the mutant animals. The mRNAs for beta3-adrenergic receptors (beta3-AR), beta2-AR and uncoupling proteins were significantly reduced in WAT and BAT from N2KO mice compared with WT mice.These studies implicate Nhlh2 in the central control of WAT and BAT function, with lack of Nhlh2 leading to adipose inflammation and altered gene expression, impaired leptin response to fasting, all suggestive of a deficient torpor response in mutant animals

Influence of different organic sources of nutrients on growth and flowering behaviour of pomegranate (Punica granatum L.) cv. Bhagwa

A field experiment on the influence of different organic sources of nutrients on growth and flowering behaviour of pomegranate (Punica granatum L.) cv. Bhagwa was conducted at College Farm, College of Horticulture, Sardarkrushinagar Dantiwada Agricultural University, Jagudan, District-Mehsana in Gujarat, India during Mrig bahar (June-January)  2017-18 and 2018-19. The present investigation was laid out in Randomized Block Design with three replications consisting of twenty-two treatments with four different organic manures viz., farmyard manure, vermicompost, poultry manure and neem cake as a source of nitrogen and recommended dose of manure and fertilizers based on plant age with or without biofertilizers (Azotobacter, PSB & KMB) and biopesticides (Trichoderma viride and Paecilomyces lilacinus). The results based on pooled data revealed that a maximum number of hermaphrodite flowers (85.17) and incomplete flowers (96.50) up to two months after treatment application, fruit set (69.45 %) along with minimum fruit drop (13.18 %) and days taken for marketable picking (168.83) were significantly (@5%) noted under treatment 100 % RDN through poultry manure + 50 ml PSB + 25 ml KMB + 5 g Trichoderma viride + 5 ml Paecilomyces lilacinus. However, significantly (@5%) highest incremental primary growth parameters viz., plant height (0.60 m), plant spread (E-W: 0.41 m and N-S: 0.43 m) and stem girth (1.47 cm) after two months of treatment application were observed under treatment 100 % RDN through vermicompost + 50 ml PSB + 25 ml KMB + 5 g Trichoderma viride + 5 ml Paecilomyces lilacinus. Thus, the organic sources viz., vermicompost and poultry manure with biofertilizers and biopesticides would be very helpful for enhancing vegetative growth and flowering behaviour of the pomegranate

Review Paper on Privacy Preservation Techniques in Cloud

In this information world, large amounts of data are collected and analyzed every day. Cloud computing is the most known model for supporting large and complex data. Organizations are moving toward cloud computing for getting benefit of its cost reduction and elasticity features but cloud computing has potential risk and vulnerabilities. One of major problem in moving to cloud computing is its security and privacy concerns.Encryption is standalone problem for the security of data stored on the cloud. So we proposed method which combines the concept of encryption along with data deduplication methodology to enhance the privacy of data over cloud. Data deduplication is a specialized data compression technique for eliminating duplicate copies of repeating data in storage. In turns this technique saves the cost and time associated with redundant accessing and processing of data overhead involve as compared to normal operations.

Adjuvant Pluronic F68 is Compatible With a Plant Root-Colonizing Probiotic, \u3ci\u3ePseudomonas chlororaphis\u3c/i\u3e O6

Plant probiotic bacteria are being increasingly used to maximize both the productivity and quality of field crops. Pseudomonas chlororaphis O6 (PcO6) is a plant root colonizer with probiotic activities. This bacterium produces an array of metabolites, including a group of phenazines that are functional in plant protection. The paper reports responses of PcO6 to a nonionic triblock copolymer surfactant, Pluronic F68. This Pluronic exhibits membrane healing activity and improves cryopreservation recovery in eukaryotic cells. The product is FDA-approved and is applied as an adjuvant in formulations used in agriculture, medicine, and biotechnology. Growth of PcO6 on lysogeny broth at 25 °C was unhindered by 0.1 and 1.0 g/L F68, reduced at 10 g/L, and with significant inhibition at 100 g/L F68; micelle formation could account for inhibited growth at higher doses. Phenazine production was not changed by F68, whereas the surfactant activity of F68 induced the spread of bacterial colonization on 0.5% agar. Exposure of cells to fluorescein-labeled F68 resulted in intense fluorescence, stable to washing, showing a direct association of the Pluronic with the bacterium. However, neither protection nor harm was found for PcO6 cells suspended in either 0.1% or 1% F68 after three freeze (–20 °C)/thaw cycles. These findings suggest that F68 could be compatible for use in agricultural formulations with little effect on probiotics such as PcO6

A Review and an Approach of Flying Electric Generators as Alternate Source of Energy

This paper presents a review of flying electric generators which are used to harness kinetic energy in powerful, persistent high altitude winds. It has been found that FEGs could give individual output of up to 40MW. It is a lighter wind turbine that rotates about a horizontal axis in response to wind, generating electrical energy. This electrical energy is transferred down for immediate use, or to a set of batteries for later use, or to the power grid. This paper presents the critical analysis of existing literature which is relevant to flying electric generator Though, the literature consists of a lot many research contributions, but, here, we have analyzed some important research and review papers. The existing approaches are categorized based on the basic concepts involved in the mechanisms. The emphasis is on the concepts used by the concerned authors, the database used for experimentations and the performance evaluation parameters. Their claims are also highlighted. Finally, the findings are summarized related to the studied and analyzed research papers. Paper concludes with the motivation behind identified problem

A Review and an Approach of Water Pollution Indication using Arduino Uno

Drinking water is critical for the wellbeing and prosperity of all people and creatures because water play major role in all living beings and most danger disease are caused by water and it is our duty to provide clean and safe water and also to monitor the pollution level in water it is additionally essential for farming utilization for good product yielding and natural way of life linkage wellbeing issues. With over 200 children dying per day due to unsafe water, drinking water crisis is ranked one on the global risk by World Economic Forum, 2015. This paper presents an easy and comprehensive methodology is microcontroller sensor based system continuous observing and pollution recognition for both drinking and non-drinking water dissemination frameworks and in addition for customer locales

VUV PROPERTIES OF Eu3+- DOPED YBO3 PHOSPHOR PREPARED VIA ALDO-KETO AND SOLID-STATE PROCESS

The Eu3+ doped YBO3 was prepared by the novel aldo-keto method. The structure and VUV luminescence properties of the title compound were studied and compared to the corresponding properties of the materials prepared by a conventional solid state reaction. The use of novel aldo-keto method in preparation lowered the reaction threshold temperature by c.a. 300oC. The YBO3 phosphor from the aldo-keto method look like evenly sized spherical structures, whereas those from the solid-state process look like some agglomerates of little spheres. The Eu3+-doped YBO3 phosphors prepared by both methods showed the red emission with peak around 592, 611 and 627 nm at excitations wavelengths 147 and172 nm, which corresponds to the transitions from the excited 5D0 level to the 7FJ (J = 1, 2, 3, 4) levels of Eu3+ activators. However, the emission intensity of the Eu3+ doped YBO3 from the aldo-keto system is about 2 times as much as that from the sold-state process

Automatic Overhead Water Tank Cleaning System: A Review and an Approach

Aim of this paper is to develop a mechanical system for cleaning domestic cylindrical water tank. The mechanical system includes two main mechanisms which are rack and pinion gear mechanism and reciprocating four bar linkage mechanism. The rack and pinion arrangement is used to move whole mechanical system up and down for cleaning the cylindrical tank. The rack is fixed on the motor and the four-bar mechanism is attached to the motor shaft. PVC brushes are attached to the ends of the four-bar linkage. Four bar linkage is made in such a way that it can be adjusted according to inside diameter of the tank. When the motor is started the linkage rotates and with the help of brushes, cleaning of wall and base of tank takes place. The purpose of this project is to reduce the human efforts and to avoid the chemical influence on health of person entering the tank for cleaning. In this modern world, cleaning of overhead tanks manually is a tedious job. To overcome this we have aimed at tackling the disadvantages of cleaning overhead tanks, so an automatic system overhead tank cleaning is designed to provide high safety, high efficiency, less time for cleaning and to avoid environmental pollution problems. Purpose of this paper is to clean domestic cylindrical water tank with the help of mechatronics system. The mechatronics system consists of a grooved gear rod attached to two arms with brushes at ends. The two arms are connected to the gear rod by nut. By rotating the gear rod, the up and down motion of the two arms is achieved. The gear rod is rotated with the help of a D.C gear motor. The main grooved shaft is powered by an A.C motor. The motor and the shaft are connected by a rubber belt. The clockwise rotation of the main shaft will make the arms move and vice versa. The whole operation is controlled by a circuit consisting of relay switches, buttons, and PIC microcontroller. The number of times for the operation to repeat can be fed into the circuit. The achievement of this project is reduction of cost and manual labour because there will be harmful diseases for the person who will go inside and it will affect the health as well as the other human being who consumes water from the tank

Growth inhibition in Rhizoetonia bataticola and Xanthomonas axonopodis pv. malvacerum by herbal oils

Oils extracted from six medicinal plants viz., mentha (Mentha arvensis), ocimum (Ocimum sanctum), lemongrass (Cympobogan flexuosus), citronella (Cympobogan winternus), turmeric (Curcuma longa) and palmarosa (Cympobogan martinii) were tested under in vitro condition for their antifungal and antibacterial properties. Mentha, ocilnum, palmarosa and lemongrass oil exhibited 100% inhibition of Rhizoctonia bataticola at 1 and 2% concentrations, whereas citronella oil recorded 100% inhibition at 2% concentration. Turmeric oil was found to be less effective against Rhizoctonia. Mentha oil showed the highest (17 mm) inhibition zone against Xanthomonas axonopodis pv. malvacerum followed by ocimum oil (12 mm). The studies showed that mentha oil possessed very high inhibitory effects on fungi and bacteria. &nbsp