How Small Firms in the High Quality Food Sector Can Improve Their Business Performance: The Ligurian Oil Case Study

This paper proposes a methodology to create market value in the high quality food sector. The research starts from the consumer’s opinion about the attributes characterizing a high quality food, then a market research shows which are the attributes able to increase the good value in the different distribution channels. The paper shows how attributes are market and channel dependent, therefore attribute able to create value in certain markets and channels are uninflected in others. The methodology proposed is concretely applied on a case study: the Italian Extra virgin olive oil sector with a particular focus on the Ligurian olive oil, one of the most appreciated Italian oil. Likewise the methodology can be implemented form many other high quality food

APCcdh1 Mediates Degradation of the Oncogenic Rho-GEF Ect2 after Mitosis

Background: Besides regulation of actin cytoskeleton-dependent functions, Rho GTPase pathways are essential to cell cycle progression and cell division. Rho, Rac and Cdc42 regulate G1 to S phase progression and are involved in cytokinesis. RhoA GDP/GTP cycling is required for normal cytokinesis and recent reports have shown that the exchange factor Ect2 and the GTPase activating protein MgcRacGAP regulate RhoA activity during mitosis. We previously showed that the transcription factors E2F1 and CUX1 regulate expression of MgcRacGAP and Ect2 as cells enter S-phase. Methodology/Principal Findings: We now report that Ect2 is subject to proteasomal degradation after mitosis, following ubiquitination by the APC/C complex and its co-activator Cdh1. A proper nuclear localization of Ect2 is necessary for its degradation. APC-Cdh1 assembles K11-linked poly-ubiquitin chains on Ect2, depending upon a stretch of,25 amino acid residues that contain a bi-partite NLS, a conventional D-box and two TEK-like boxes. Site-directed mutagenesis of target sequences generated stabilized Ect2 proteins. Furthermore, such degradation-resistant mutants of Ect2 were found to activate RhoA and subsequent signalling pathways and are able to transform NIH3T3 cells. Conclusions/Significance: Our results identify Ect2 as a bona fide cell cycle-regulated protein and suggest that its ubiquitination-dependent degradation may play an important role in RhoA regulation at the time of mitosis. Our findings raise the possibility that the overexpression of Ect2 that has been reported in some human tumors might result not only from deregulated transcription, but also from impaired degradation

Saccharomyces boulardii Improves Intestinal Cell Restitution through Activation of the α2β1 Integrin Collagen Receptor

Intestinal epithelial cell damage is frequently seen in the mucosal lesions of inflammatory bowel diseases such as ulcerative colitis or Crohn's disease. Complete remission of these diseases requires both the cessation of inflammation and the migration of enterocytes to repair the damaged epithelium. Lyophilized Saccharomyces boulardii (Sb, Biocodex) is a nonpathogenic yeast widely used as a therapeutic agent for the treatment and prevention of diarrhea and other gastrointestinal disorders. In this study, we determined whether Sb could accelerate enterocyte migration. Cell migration was determined in Sb force-fed C57BL6J mice and in an in vitro wound model. The impact on α2β1 integrin activity was assessed using adhesion assays and the analysis of α2β1 mediated signaling pathways both in vitro and in vivo. We demonstrated that Sb secretes compounds that enhance the migration of enterocytes independently of cell proliferation. This enhanced migration was associated with the ability of Sb to favor cell-extracellular matrix interaction. Indeed, the yeast activates α2β1 integrin collagen receptors. This leads to an increase in tyrosine phosphorylation of cytoplasmic molecules, including focal adhesion kinase and paxillin, involved in the integrin signaling pathway. These changes are associated with the reorganization of focal adhesion structures. In conclusion Sb secretes motogenic factors that enhance cell restitution through the dynamic regulation of α2β1 integrin activity. This could be of major importance in the development of novel therapies targeting diseases characterized by severe mucosal injury, such as inflammatory and infectious bowel diseases

Complexity and dynamics of the winemaking bacterial communities in berries, musts, and wines from apulian grape cultivars through time and space

Currently, there is very little information available regarding the microbiome associated with the wine production chain. Here, we used an amplicon sequencing approach based on high-throughput sequencing (HTS) to obtain a comprehensive assessment of the bacterial community associated with the production of three Apulian red wines, from grape to final product. The relationships among grape variety, the microbial community, and fermentation was investigated. Moreover, the winery microbiota was evaluated compared to the autochthonous species in vineyards that persist until the end of the winemaking process. The analysis highlighted the remarkable dynamics within the microbial communities during fermentation. A common microbial core shared among the examined wine varieties was observed, and the unique taxonomic signature of each wine appellation was revealed. New species belonging to the genus Halomonas were also reported. This study demonstrates the potential of this metagenomic approach, supported by optimized protocols, for identifying the biodiversity of the wine supply chain. The developed experimental pipeline offers new prospects for other research fields in which a comprehensive view of microbial community complexity and dynamics is desirable.Peer ReviewedPostprint (published version

Anthroprometrical profile of cüban volleyball scholar athletes.

Durante la celebración de los pasados Juegos Nacionales Escolares efecutados en la Habana se realizó un estudio con 116 atletas escolares de todo el país, controlando 18 variables antropométricas, determinándose la talla, el peso la edad morfológica, la predicción de crecimiento de la talla, la menarquía, la composición corporal y el somatotipo, ya que se encuentran dentro de los parámetros más importantes en las jugadoras escolares de Voleibol, tanto para su selección como para la realización del entrenamiento deportivo. Los resultados del trabajo aportan tablas percentilares de la talla, el peso, la composición corporal y el somatotipo para las edades de 12 y 13 años. Los mismo permitieron apreciar la importancia de los indicadores estudiados y valorar el perfil antropométricos como una forma a utilizar en la selección de talentos deportivos por nuestros entrenadores.A study of 116 scholar athletes of the whole country was done during the last National Scholar Games, controlling 18 anthroprometrical variables, determining size, weight, morphological age, growing size prediction, menarquy, body composition and somatotype, since they form part of the most important parameters for both selection and sportive training in volleyball scholar players. As a result percentilar tables of weight, size, body composition and somatotype are presented which may estímate the importance of the studied indicators and valué the anthroprometrical profile as a way to be used un the selection of sport talents by our trainers.Incluye referencias bibliográfica

Physical and physiological heterogeneity within grape bunch: Impact on mechanical properties

National audienc