2,274 research outputs found

    High resolution synchrotron imaging of wheat root hairs growing in soil and image based modelling of phosphate uptake

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    Root hairs are known to be highly important for uptake of sparingly soluble nutrients, particularly in nutrient deficient soils. Development of increasingly sophisticated mathematical models has allowed uptake characteristics to be quantified. However, modelling has been constrained by a lack of methods for imaging live root hairs growing in real soils.We developed a plant growth protocol and used Synchrotron Radiation X-ray Tomographic Microscopy (SRXTM) to uncover the 3D interactions of root hairs in real soil. We developed a model of phosphate uptake by root hairs based directly on the geometry of hairs and associated soil pores as revealed by imaging.Previous modelling studies found that root hairs dominate phosphate uptake. By contrast, our study suggests that hairs and roots contribute equally. We show that uptake by hairs is more localised than by roots and strongly dependent on root hair and aggregate orientation.The ability to image hair-soil interactions enables a step change in modelling approaches, allowing a more realistic treatment of processes at the scale of individual root hairs in soil pores

    Tidal signals in ocean-bottom magnetic measurements of the Northwestern Pacific: observation versus prediction

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    Motional induction in the ocean by tides has long been observed by both land and satellite measurements of magnetic fields. While these signals are weak (∼10 nT) when compared to the main magnetic field, their persistent nature makes them important for consideration during geomagnetic field modelling. Previous studies have reported several discrepancies between observations and numerical predictions of the tidal magnetic signals and those studies were inconclusive of the source of the error. We address this issue by (1) analysing magnetometer data from ocean-bottom stations, where the low-noise and high-signal environment is most suitable for detecting the weak tidal magnetic signals, (2) by numerically predicting the magnetic field with a spatial resolution that is 16times higher than the previous studies and (3) by using four different models of upper-mantle conductivity. We use vector magnetic data from six ocean-bottom electromagnetic (OBEM) stations located in the Northwestern Pacific Ocean. The OBEM tidal amplitudes were derived using an iteratively re-weighted least-squares (IRLS) method and by limiting the analysis of lunar semidiurnal (M2), lunar elliptic semidinurnal (N2) and diurnal (O1) tidal modes to the night-time. Using a 3-D electromagnetic induction solver and the TPX07.2 tidal model, we predict the tidal magnetic signal. We use earth models with non-uniform oceans and four 1-D mantle sections underneath taken from Kuvshinov and Olsen, Shimizu etal. and Baba etal. to compare the effect of upper-mantle conductivity. We find that in general, the predictions and observations match within 10-70 per cent across all the stations for each of the tidal modes. The median normalized percent difference (NPD) between observed and predicted amplitudes for the tidal modes M2, N2 and O1 were 15 per cent, 47 per cent and 98 per cent, respectively, for all the stations and models. At the majority of stations, and for each of the tidal modes, the higher resolution (0.25°×0.25°) modelling gave amplitudes consistently closer to the observations than the lower resolution (1°×1°) modelling. The difference in lithospheric resistance east and west of the Izu-Bonin trench system seems to be affecting the model response and observations in the O1 tidal mode. This response is not seen in the M2 and N2 modes, thereby indicating that the O1 mode is more sensitive to lithospheric resistanc

    Inhibition of protein crystallization by evolutionary negative design

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    In this perspective we address the question: why are proteins seemingly so hard to crystallize? We suggest that this is because of evolutionary negative design, i.e. proteins have evolved not to crystallize, because crystallization, as with any type of protein aggregation, compromises the viability of the cell. There is much evidence in the literature that supports this hypothesis, including the effect of mutations on the crystallizability of a protein, the correlations found in the properties of crystal contacts in bioinformatics databases, and the positive use of protein crystallization by bacteria and viruses.Comment: 5 page

    Boosting Performance of Data-intensive Analysis Workflows with Distributed Coordinated Caching

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    Data-intensive end-user analyses in high energy physics require high data throughput to reach short turnaround cycles. This leads to enormous challenges for storage and network infrastructure, especially when facing the tremendously increasing amount of data to be processed during High-Luminosity LHC runs. Including opportunistic resources with volatile storage systems into the traditional HEP computing facilities makes this situation more complex. Bringing data close to the computing units is a promising approach to solve throughput limitations and improve the overall performance. We focus on coordinated distributed caching by coordinating workows to the most suitable hosts in terms of cached files. This allows optimizing overall processing efficiency of data-intensive workows and efficiently use limited cache volume by reducing replication of data on distributed caches. We developed a NaviX coordination service at KIT that realizes coordinated distributed caching using XRootD cache proxy server infrastructure and HTCondor batch system. In this paper, we present the experience gained in operating coordinated distributed caches on cloud and HPC resources. Furthermore, we show benchmarks of a dedicated high throughput cluster, the Throughput-Optimized Analysis-System (TOpAS), which is based on the above-mentioned concept

    Satellite tidal magnetic signals constrain oceanic lithosphere-asthenosphere boundary

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    The tidal flow of electrically conductive oceans through the geomagnetic field results in the generation of secondary magnetic signals, which provide information on the subsurface structure. Data from the new generation of satellites were shown to contain magnetic signals due to tidal flow; however, there are no reports that these signals have been used to infer subsurface structure. We use satellite-detected tidal magnetic fields to image the global electrical structure of the oceanic lithosphere and upper mantle down to a depth of about 250 km. The model derived from more than 12 years of satellite data reveals a ≈72-km-thick upper resistive layer followed by a sharp increase in electrical conductivity likely associated with the lithosphere-asthenosphere boundary, which separates colder rigid oceanic plates from the ductile and hotter asthenosphere.ISSN:2375-254

    Dynamic Resource Extension for Data Intensive Computing with Specialized Software Environments on HPC Systems

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    Modern High Energy Physics (HEP) requires large-scale processing of extensive amounts of scientific data. The needed computing resources are currently provided statically by HEP specific computing centers. To increase the number of available resources, for example to cover peak loads, the HEP computing development team at KIT concentrates on the dynamic integration of additional computing resources into the HEP infrastructure. Therefore, we developed ROCED, a tool to dynamically request and integrate computing resources including resources at HPC centers and commercial cloud providers. Since these resources usually do not support HEP software natively, we rely on virtualization and container technologies, which allows us to run HEP workflows on these so called opportunistic resources. Additionally, we study the efficient processing of huge amounts of data on a distributed infrastructure, where the data is usually stored at HEP specific data centers and is accessed remotely over WAN. To optimize the overall data throughput and to increase the CPU efficiency, we are currently developing an automated caching system for frequently used data that is transparently integrated into the distributed HEP computing infrastructure

    Federation of compute resources available to the German CMS community

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    The German CMS community (DCMS) as a whole can benefit from the various compute resources, available to its different institutes. While Grid-enabled and National Analysis Facility resources are usually shared within the community, local and recently enabled opportunistic resources like HPC centers and cloud resources are not. Furthermore, there is no shared submission infrastructure available. Via HTCondor\u27s [1] mechanisms to connect resource pools, several remote pools can be connected transparently to the users and therefore used more efficiently by a multitude of user groups. In addition to the statically provisioned resources, also dynamically allocated resources from external cloud providers as well as HPC centers can be integrated. However, the usage of such dynamically allocated resources gives rise to additional complexity. Constraints on access policies of the resources, as well as workflow necessities have to be taken care of. To maintain a well-defined and reliable runtime environment on each resource, virtualization and containerization technologies such as virtual machines, Docker, and Singularity, are used
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