Comparison of two commercial ELISA kits and magnetic stirrer method for detection of Trichinella spp. in a pig slaughterhouse

ELISA represents a useful rapid method to detect the presence of specific antibodies on serum, plasma or meat juice collected at slaughter, however, false- and positive-results may occur depending on the sensitivity and specificity of the test. In this study we compare two commercial ELISA kits for the detection of specific antibodies against Trichinella spp. with respect to the gold standard method (artificial digestion) in a pig slaughterhouse. A total of 709 Iberian pigs belonging to 79 free-range herds were randomly selected and sampled (five to ten animals/herd) (Win Episcope 2.0; 95% confidence level, 8% accepted error)

Quid: observatorio de medios

En la primera parte de la obra se aborda el tema de medios y las elecciones de 2009; en la segunda, la transparencia y la rendición de cuentas, y en la tercera parte los medios de comunicación y el periodismo. Al final se presentan sembalnzas de Rigo Mora, Álvaro González de Mendoza y David “el Negro” Guerrero, quienes desde el cine y la radiodifusión, dejaron momentos memorables a la comunidad tapatía.ITESO, A.C

Comparison of two commercial ELISA kits and magnetic stirrer method for detection of Trichinella spp. in a pig slaughterhouse

ELISA represents a useful rapid method to detect the presence of specific antibodies on serum, plasma or meat juice collected at slaughter, however, false- and positive-results may occur depending on the sensitivity and specificity of the test. In this study we compare two commercial ELISA kits for the detection of specific antibodies against Trichinella spp. with respect to the gold standard method (artificial digestion) in a pig slaughterhouse. A total of 709 Iberian pigs belonging to 79 free-range herds were randomly selected and sampled (five to ten animals/herd) (Win Episcope 2.0; 95% confidence level, 8% accepted error).</p

Isolation of Salmonella spp. in pigs during transport, lairage, slaughterline and quartering

The aim of this study was to determine the prevalence of Salmonella in different points of the chain (transport, lairage, slaughterline and quartering). From eight consecutive assays a total of 134 Salmonella isolates out of 1180 different samples (11.3%) were recovered. The highest percentage of isolates were detected at the point of pre-scalding (30/80, 37.5%), caecal contents (18/80, 22.5%), trucks (13/60, 21.6%), tonsils (15/80, 18.7%), ileocaecal lymph nodes (13/80, 16.2%) and lairage (9/80, 11.2%).</p