Trends in hydroidomedusan research from 1911 to 1997

The papers on hydroidomedusae published from 1911 to 1997 total 10,934. They have been assigned to the following categories: faunistics and systematics; sub-organismal biology; ecology; evolution; life cycles; paleontology. The general trend, comprising all papers, can be divided into four time intervals: the first (1911-1939) with an average of sixty papers/year and with a slight decrease due to First World War; the second one (1940-1947), with an average of 38 papers/year, marked by a dramatic decrease coinciding with Second World War; the period 1948-1991 shows a steady increase until the mid-Seventies, when a small decrease occurred, followed by an increasing trend reaching its apex in the late Eighties-early Ninenties with a record of 296 papers in 1991 and with an average of 175 papers/year; the period 1992 1997, with an average of 178 papers/year, is marked by a sharp decrease, reaching the values of the mid Sixties. The most important category in terms of number of papers is sub-organismal biology, followed by faunistics and systematics. Systematic studies dictated the trend in the first decades of the century, whereas sub-organismal ones are prevalent from the Sixties onwards. Faunistic and systematic-taxonomic papers have a steady trend of production, with just a slight decrease over these last years. The formerly leading countries in systematics (UK, USA, France) are now almost inactive in this discipline, whereas countries with little or no tradition in this field (such as Spain) are taking the leadership

Nondecaying Hydrodynamic Interactions along Narrow Channels.

Particle-particle interactions are of paramount importance in every multibody system as they determine the collective behavior and coupling strength. Many well-known interactions such as electrostatic, van der Waals, or screened Coulomb interactions, decay exponentially or with negative powers of the particle spacing r. Similarly, hydrodynamic interactions between particles undergoing Brownian motion decay as 1/r in bulk, and are assumed to decay in small channels. Such interactions are ubiquitous in biological and technological systems. Here we confine two particles undergoing Brownian motion in narrow, microfluidic channels and study their coupling through hydrodynamic interactions. Our experiments show that the hydrodynamic particle-particle interactions are distance independent in these channels. This finding is of fundamental importance for the interpretation of experiments where dense mixtures of particles or molecules diffuse through finite length, water-filled channels or pore networks.U.F.K. was supported by an ERC starting grant (PassMembrane 261101). S.P. acknowledges funding from a Leverhulme Early Career Fellowship. K.M. was supported by a grant from the EPSRC. E.L. was supported by Marie Curie CIG grant from EU.This is the author accepted manuscript. The final version is available from APS via http://dx.doi.org/10.1103/PhysRevLett.115.03830

Versatile multiplexed super-resolution imaging of nanostructures by Quencher-Exchange-PAINT

This is the final version of the article. Available from Springer Verlag via the DOI in this record.The optical super-resolution technique DNA-PAINT (Point Accumulation Imaging in Nanoscale Topography) provides a flexible way to achieve imaging of nanoscale structures at ∼10-nanometer resolution. In DNA-PAINT, fluorescently labeled DNA “imager” strands bind transiently and with high specificity to complementary target “docking” strands anchored to the structure of interest. The localization of single binding events enables the assembly of a super-resolution image, and this approach effectively circumvents photobleaching. The solution exchange of imager strands is the basis of Exchange-PAINT, which enables multiplexed imaging that avoids chromatic aberrations. Fluid exchange during imaging typically requires specialized chambers or washes, which can disturb the sample. Additionally, diffusional washout of imager strands is slow in thick samples such as biological tissue slices. Here, we introduce Quencher-Exchange-PAINT—a new approach to Exchange-PAINT in regular open-top imaging chambers—which overcomes the comparatively slow imager strand switching via diffusional imager washout. Quencher-Exchange-PAINT uses “quencher” strands, i.e., oligonucleotides that prevent the imager from binding to the targets, to rapidly reduce unwanted single-stranded imager concentrations to negligible levels, decoupled from the absolute imager concentration. The quencher strands contain an effective dye quencher that reduces the fluorescence of quenched imager strands to negligible levels. We characterized Quencher-Exchange-PAINT when applied to synthetic, cellular, and thick tissue samples. Quencher-Exchange-PAINT opens the way for efficient multiplexed imaging of complex nanostructures, e.g., in thick tissues, without the need for washing steps. [Figure not available: see fulltext.]The work was supported by funding from the Human Frontier Science Program (No. 0027/2013) and the Engineering and Physical Sciences Research Council of the UK (No. EP/N008235/1)

Strangeness in Astrophysics and Cosmology

Some recent developments concerning the role of strange quark matter for astrophysical systems and the QCD phase transition in the early universe are addressed. Causality constraints of the soft nuclear equation of state as extracted from subthreshold kaon production in heavy-ion collisions are used to derive an upper mass limit for compact stars. The interplay between the viscosity of strange quark matter and the gravitational wave emission from rotation-powered pulsars are outlined. The flux of strange quark matter nuggets in cosmic rays is put in perspective with a detailed numerical investigation of the merger of two strange stars. Finally, we discuss a novel scenario for the QCD phase transition in the early universe, which allows for a small inflationary period due to a pronounced first order phase transition at large baryochemical potential.Comment: 8 pages, invited talk given at the International Conference on Strangeness in Quark Matter (SQM2009), Buzios, Brasil, September 28 - October 2, 200

5-FU targets rpL3 to induce mitochondrial apoptosis via cystathionine-b-synthase in colon cancer cells lacking p53

Recent findings revealed in cancer cells novel stress response pathways, which in response to many chemotherapeutic drugs causing nucleolar stress, will function independently from tumor protein p53 (p53) and still lead to cell cycle arrest and/or apoptosis. Since it is known that most cancers lack functional p53, it is of great interest to explore these emerging molecular mechanisms. Here, we demonstrate that nucleolar stress induced by 5-fluorouracil (5-FU) in colon cancer cells devoid of p53 leads to the activation of ribosomal protein L3 (rpL3) as proapoptotic factor. rpL3, as ribosome-free form, is a negative regulator of cystathionine-β-synthase (CBS) expression at transcriptional level through a molecular mechanism involving Sp1. The rpL3-CBS association affects CBS stability and, in addition, can trigger CBS translocation into mitochondria. Consequently apoptosis will be induced through the mitochondrial apoptotic cell death pathway characterized by an increased ratio of Bax to Bcl-2, cytochrome c release and subsequent caspase activation. It is noteworthy that silencing of CBS is associated to a strong increase of 5-FU-mediated inhibition of cell migration and proliferation. These data reveal a novel mechanism to accomplish p53-independent apoptosis and suggest a potential therapeutic approach aimed at upregulating rpL3 for treating cancers lacking p53

Single cell imaging of nuclear architecture changes

This is the final version. Available from Frontiers Media via the DOI in this record.Data and materials availability: Data obtained in this work are available upon request.The dynamic architecture of chromatin, the macromolecular complex comprised primarily of DNA and histones, is vital for eukaryotic cell growth. Chemical and conformational changes to chromatin are important markers of functional and developmental processes in cells. However, chromatin architecture regulation has not yet been fully elucidated. Therefore, novel approaches to assessing chromatin changes at the single-cell level are required. Here we report the use of FTIR imaging and microfluidic cell-stretcher chips to assess changes to chromatin architecture and its effect on the mechanical properties of the nucleus in immune cells. FTIR imaging enables label-free chemical imaging with subcellular resolution. By optimizing the FTIR methodology and couple it with cell segmentation analysis approach, we have identified key spectral changes corresponding to changes in DNA levels and chromatin conformation at the single cell level. By further manipulating live single cells using pressure-driven microfluidics, we found that chromatin decondensation – either during general transcriptional activation or during specific immune cell maturation – can ultimately lead to nuclear auxeticity which is a new biological phenomenon recently identified. Taken together our findings demonstrate the tight and, potentially bilateral, link between extra-cellular mechanotransduction and intra-cellular nuclear architecture.Engineering and Physical Sciences Research Council (EPSRC)Biotechnology and Biological Sciences Research Council (BBSRC)Academy of Medical SciencesRoyal Societ

Survival patterns of Dekkera bruxellensis in wines and inhibitory effect of sulphur dioxide

The wine spoilage yeast species Dekkera bruxellensis, after inoculation in red wines, displayed three survival patterns characterized by: i) initial lag phase followed by growth and sequential death; ii) initial death phase leading to reduced viable counts followed by growth and sequential death; and iii) death phase leading to complete loss of viability. These survival patterns were observed for the same strain in different dry red wine blends with 12% (v/v) ethanol and pH 3.50, in the absence of free sulphur dioxide. For the same wine blend, these patterns also varied with the tested strain. Under laboratory conditions the addition of 150 mg/l of potassium metabisulphite (PMB) to dry red wine with 12% (v/v) ethanol and pH 3.50 reduced initial cell counts by more than 6 logarithmic cycles, inducing full death within less than 24 h. Winery trials showed that D. bruxellensis blooms were only prevented in the presence of about 40 mg/l of free sulphur dioxide in dry red wine, with 13.8% (v/v) ethanol and pH 3.42, matured in oak barrels. These different amounts of PMB and sulphur dioxide corresponded to about 1 mg/l of molecular sulphur dioxide. Our results therefore demonstrate that the control of populations of D. bruxellensis growing in red wine can only be achieved under the presence of relatively high doses of molecular sulphur dioxide

Equation of state at high densities and modern compact star observations

Recently, observations of compact stars have provided new data of high accuracy which put strong constraints on the high-density behaviour of the equation of state of strongly interacting matter otherwise not accessible in terrestrial laboratories. The evidence for neutron stars with high mass (M =2.1 +/- 0.2 M_sun for PSR J0751+1807) and large radii (R > 12 km for RX J1856-3754) rules out soft equations of state and has provoked a debate whether the occurence of quark matter in compact stars can be excluded as well. In this contribution it is shown that modern quantum field theoretical approaches to quark matter including color superconductivity and a vector meanfield allow a microscopic description of hybrid stars which fulfill the new, strong constraints. The deconfinement transition in the resulting stiff hybrid equation of state is weakly first order so that signals of it have to be expected due to specific changes in transport properties governing the rotational and cooling evolution caused by the color superconductivity of quark matter. A similar conclusion holds for the investigation of quark deconfinement in future generations of nucleus-nucleus collision experiments at low temperatures and high baryon densities such as CBM @ FAIR.Comment: 6 pages, 2 figures, accepted for publication in J. Phys. G. (Special Issue

Strange Exotic States and Compact Stars

We discuss the possible appearance of strange exotic multi-quark states in the interior of neutron stars and signals for the existence of strange quark matter in the core of compact stars. We show how the in-medium properties of possible pentaquark states are constrained by pulsar mass measurements. The possibility of generating the observed large pulsar kick velocities by asymmetric emission of neutrinos from strange quark matter in magnetic fields is outlined.Comment: 10 pages, invited talk given at the International Conference on Strangeness in Quark Matter 2006 (SQM2006), UCLA, USA, March 26-31, 2006, Journal of Physics G in press, refs. adde

Optical properties of in-vitro biomineralised silica

This is the final version of the article. Available from the publisher via the DOI in this record.Silicon is the second most common element on the Earth's crust and its oxide (SiO(2)) the most abundant mineral. Silica and silicates are widely used in medicine and industry as well as in micro- and nano-optics and electronics. However, the fabrication of glass fibres and components requires high temperature and non-physiological conditions, in contrast to biosilica structures in animals and plants. Here, we show for the first time the use of recombinant silicatein-α, the most abundant subunit of sponge proteins catalyzing biosilicification reactions, to direct the formation of optical waveguides in-vitro through soft microlithography. The artificial biosilica fibres mimic the natural sponge spicules, exhibiting refractive index values suitable for confinement of light within waveguides, with optical losses in the range of 5-10 cm(-1), suitable for application in lab-on-chips systems. This method extends biosilicification to the controlled fabrication of optical components by physiological processing conditions, hardly addressed by conventional technologies.This work is financially supported by the BIO-LITHO European project (6th Framework Program, NMP). W.E.G. Muller is holder of an ERC Advanced Research Grant