Characterisation of DOG-1 expression in salivary gland tumours and comparison with myoepithelial markers

DOG1 is an established diagnostic marker for gastrointestinal stromal tumours (GIST), but has been reported in salivary gland tumours (SGT) as an acinar and intercalated duct marker. However, its specificity and distribution is not well established. The aim of this study was to evaluate the diagnostic utility of DOG-1 expression in SGT in addition to comparing it with myoepithelial markers. Normal salivary tissue and SGT (n = 184) were examined for expression of DOG1 and a range of myoepithelial markers. SGT included: acinic cell carcinoma (ACC, n = 15), secretory carcinoma (SC, n = 9), pleomorphic adenoma (PA, n = 49), carcinoma ex-PA (Ca ex-PA, n = 11), adenoid cystic carcinoma (AdCC, n = 20), polymorphous adenocarcinoma (PAC, n = 6), myoepithelioma (n = 6), myoepithelial carcinoma (MC, n = 2), basal cell adenoma (BCA, n = 14), canalicular adenoma (CA, n = 19), mucoepidermoid carcinoma (MEC, n = 11), oncocytoma (n = 2), adenocarcinoma NOS (AdNOS, n = 4), basal cell adenocarcinoma (BCAC, n = 2), salivary duct carcinoma (SDC, n = 3) and papillary cystadenocarcinoma (PCAC, n = 1). Normal acini and ACC (14/15) showed strong luminal DOG1 staining; SC were largely negative with only focal expression in 3/9 cases. Luminal staining was seen in PA (14/49), PAC (4/6), Ca ex-PA (4/11) and AdCC (6/20). 8/11 MEC showed luminal and/or mucous cell staining. No staining was seen in myoepithelioma, MC, CA, adNOS and BCAC. BCA showed strong staining of myoepithelial cells in some cases (5/14). Variable myoepithelial DOG1 staining was seen in PA, Ca ex PA, BCA, SDC and PCAC which was not as consistent as myoepithelial markers such as calponin, p63 and αSMA. Absence of DOG1 can differentiate ACC from SC, but staining is variable in PA, PLGA and Ca ex-PA. Myoepithelial staining in some tumours but not in normal gland suggests a wider distribution in SGT than originally envisaged

Comparative effectiveness of mobile health smoking cessation approaches among underserved patients in primary care: Study protocol for the PROMOTE-UP trial

Tobacco smoking is the leading cause of preventable disease, disability, and premature death in the United States. Recent advances have led to two efficacious mobile health (mHealth) treatments for smoking cessation: iCanQuit, an Acceptance and Commitment Therapy-based behavioral treatment promoting cessation through accepting triggers and committing to values; and Motiv8, a contingency management intervention promoting smoking cessation with financial incentives via biochemically verified abstinence. This study will evaluate the comparative effectiveness of the Florida Quitline, iCanQuit alone, and iCanQuit+Motiv8 in a pragmatic trial among patients who smoke in underserved primary care settings. The study will be an individually-randomized controlled trial with three arms (Florida Quitline, iCanQuit alone, iCanQuit+Motiv8 combined) conducted in multiple primary care practices affiliated with the OneFlorida+ Clinical Research Consortium. Adult patients who smoke will be randomized to one of the 3 study arms (n = 444/arm), stratified by healthcare setting (academic vs. community). The primary outcome will be 7-day point prevalence smoking abstinence at 6 months post-randomization. Secondary outcomes will be 12-month smoking abstinence, patient satisfaction with the interventions, and changes in patient quality of life and self-efficacy. The study will also assess how and for whom the interventions help sub-group patients in achieving smoking abstinence by measuring theory-derived factors that mediate smoking outcome-specific baseline moderators. Results from this study will provide evidence for the comparative effectiveness of mHealth smoking cessation interventions in healthcare settings. Use of mHealth interventions can make smoking cessation resources more equitably accessible and have far-reaching impact on community and population health. ClinicalTrials.gov, NCT05415761, Registered 13 June 2022

Reproduction of Cichla kelberi Kullander and Ferreira, 2006 introduced into an artificial lake in southeastern Brazil

The objective of this study was to characterize the reproduction of Cichla kelberi in an artificial lake, located in the municipality of Leme, in the state of São Paulo, Brazil. Breeding occurred in spring, and summer. The L50 and the L100 of this species were 192 and 235 mm (L50), for males and females, respectively, and 290 mm (L100) for both sexes. Spawning was parceled. The oocytes matured at a size of 428.4 µm, reaching their maximum at 2,203.2 µm. A mean of fecundity were of 12,129.2 oocytes, with the mean of oocytes in each batch of 4,897.7. This species has external fertilization, is nonmigratory, and with parental care of the young. Various attributes of the peacock bass make their introduction a temptation. However, due to their feeding and reproductive characteristics, they have no natural predators, making it difficult to control their population growth or eradicate them

Dielectrical, optical, and structural characterization of TiO2/PVA nanocomposite films for dielectric applications

In this study, titanium dioxide/poly(vinyl) alcohol (TiO2/PVA) nanocomposite thin films were prepared by a simple spin-coating method for dielectric applications. Structural, morphological, optical, and dielectric properties of samples were analyzed by XRD, SEM, AFM, UV-VIS, and dielectric measurements. The effect of TiO2 nanoparticles on these properties was investigated. It was found that TiO2 nanoparticles lead to increase the crystallinity of nanocomposites. As TiO2 content in the composite structure increases, while average transmittance values decrease, reflectance values increase. The dielectric parameters such as real and imaginary part of complex permittivity which are related to the stored energy and the dissipation (or loss) of energy were significantly affected by the presence of TiO2 nanoparticles. In addition, alternating current (A.C.) conductivity increased with increasing applied frequency. The A.C. conductivity also confirmed that all the samples exhibited the insulator behavior and obeyed the universal power law