541 research outputs found
Sampling Distributions of Random Electromagnetic Fields in Mesoscopic or Dynamical Systems
We derive the sampling probability density function (pdf) of an ideal
localized random electromagnetic field, its amplitude and intensity in an
electromagnetic environment that is quasi-statically time-varying statistically
homogeneous or static statistically inhomogeneous. The results allow for the
estimation of field statistics and confidence intervals when a single spatial
or temporal stochastic process produces randomization of the field. Results for
both coherent and incoherent detection techniques are derived, for Cartesian,
planar and full-vectorial fields. We show that the functional form of the
sampling pdf depends on whether the random variable is dimensioned (e.g., the
sampled electric field proper) or is expressed in dimensionless standardized or
normalized form (e.g., the sampled electric field divided by its sampled
standard deviation). For dimensioned quantities, the electric field, its
amplitude and intensity exhibit different types of
Bessel sampling pdfs, which differ significantly from the asymptotic
Gauss normal and ensemble pdfs when is relatively
small. By contrast, for the corresponding standardized quantities, Student ,
Fisher-Snedecor and root- sampling pdfs are obtained that exhibit
heavier tails than comparable Bessel pdfs. Statistical uncertainties
obtained from classical small-sample theory for dimensionless quantities are
shown to be overestimated compared to dimensioned quantities. Differences in
the sampling pdfs arising from de-normalization versus de-standardization are
obtained.Comment: 12 pages, 15 figures, accepted for publication in Phys. Rev. E, minor
typos correcte
Asymmetry Function of Interstellar Scintillations of Pulsars
A new method for separating intensity variations of a source's radio emission
having various physical natures is proposed. The method is based on a joint
analysis of the structure function of the intensity variations and the
asymmetry function, which is a generalization of the asymmetry coefficient and
characterizes the asymmetry of the distribution function of the intensity
fluctuations on various scales for the inhomogeneities in the diffractive
scintillation pattern. Relationships for the asymmetry function in the cases of
a logarithmic normal distribution of the intensity fluctuations and a normal
distribution of the field fluctuations are derived. Theoretical relationships
and observational data on interstellar scintillations of pulsars (refractive,
diffractive, and weak scintillations) are compared. Pulsar scintillations match
the behavior expected for a normal distribution of the field fluctuations
(diffractive scintillation) or logarithmic normal distribution of the intensity
fluctuations (refractive and weak scintillation). Analysis of the asymmetry
function is a good test for distinguishing scintillations against the
background of variations that have different origins
Immunolocalization of dually phosphorylated MAPKs in dividing root meristem cells of Vicia faba, Pisum sativum, Lupinus luteus and Lycopersicon esculentum
Key message In plants, phosphorylated MAPKs display
constitutive nuclear localization; however, not all
studied plant species show co-localization of activated
MAPKs to mitotic microtubules.
Abstract The mitogen-activated protein kinase (MAPK)
signaling pathway is involved not only in the cellular
response to biotic and abiotic stress but also in the regulation
of cell cycle and plant development. The role of
MAPKs in the formation of a mitotic spindle has been
widely studied and the MAPK signaling pathway was
found to be indispensable for the unperturbed course of cell
division. Here we show cellular localization of activated
MAPKs (dually phosphorylated at their TXY motifs) in
both interphase and mitotic root meristem cells of Lupinus
luteus, Pisum sativum, Vicia faba (Fabaceae) and Lycopersicon esculentum (Solanaceae). Nuclear localization
of activated MAPKs has been found in all species. Colocalization
of these kinases to mitotic microtubules was
most evident in L. esculentum, while only about 50 % of
mitotic cells in the root meristems of P. sativum and V.
faba displayed activated MAPKs localized to microtubules
during mitosis. Unexpectedly, no evident immunofluorescence
signals at spindle microtubules and phragmoplast
were noted in L. luteus. Considering immunocytochemical
analyses and studies on the impact of FR180204 (an
inhibitor of animal ERK1/2) on mitotic cells, we hypothesize
that MAPKs may not play prominent role in the
regulation of microtubule dynamics in all plant species
Airway surface liquid depth imaged by surface laser reflectance microscopy
The thin layer of liquid at the surface of airway epithelium, the airway surface liquid (ASL), is important in normal airway physiology and in the pathophysiology of cystic fibrosis. At present, the best method to measure ASL depth involves scanning confocal microscopy after staining with an aqueous-phase fluorescent dye. We describe here a simple, noninvasive imaging method to measure ASL depth by reflectance imaging of an epithelial mucosa in which the surface is illuminated at a 45-degree angle by an elongated 13-µm wide rectangular beam produced by a 670-nm micro-focus laser. The principle of the method is that air–liquid, liquid–liquid, and liquid–cell interfaces produce distinct specular or diffuse reflections that can be imaged to give a micron-resolution replica of the mucosal surface. The method was validated using fluid layers of specified thicknesses and applied to measure ASL depth in cell cultures and ex vivo fragments of pig trachea. In addition, the method was adapted to measure transepithelial fluid transport from the dynamics of fluid layer depth. Compared with confocal imaging, ASL depth measurement by surface laser reflectance microscopy does not require dye staining or costly instrumentation, and can potentially be adapted for in vivo measurements using fiberoptics
PsRBR1 encodes a pea retinoblastoma-related protein that is phosphorylated in axillary buds during dormancy-to-growth transition
In intact plants, cells in axillary buds are arrested at the G1 phase of the cell cycle during dormancy. In mammalian cells, the cell cycle is suppressed at the G1 phase by the activities of retinoblastoma tumor suppressor gene (RB) family proteins, depending on their phosphorylation state. Here, we report the isolation of a pea cDNA clone encoding an RB-related protein (PsRBR1, Accession No. AB012024) with a high degree of amino acid conservation in comparison with RB family proteins. PsRBR1 protein was detected as two polypeptides using an anti-PsRBR1 antibody in dormant axillary buds, whereas it was detected as three polypeptides, which were the same two polypeptides and another larger polypeptide 2 h after terminal decapitation. Both in vitro-synthesized PsPRB1 protein and lambda protein phosphatase-treated PsRBR1 protein corresponded to the smallest polypeptide detected by anti-PsRBR1 antibody, suggesting that the three polypeptides correspond to non-phosphorylated form of PsRBR1 protein, and lower- and higher-molecular mass forms of phosphorylated PsRBR1 protein. Furthermore, in vivo labeling with [32P]-inorganic phosphate indicated that PsRBR1 protein was more phosphorylated before mRNA accumulation of cell cycle regulatory genes such as PCNA. Together these findings suggest that dormancy-to-growth transition in pea axillary buds is regulated by molecular mechanisms of cell cycle control similar to those in mammals, and that the PsRBR1 protein has an important role in suppressing the cell cycle during dormancy in axillary buds
The Dark Side of the Salad: Salmonella typhimurium Overcomes the Innate Immune Response of Arabidopsis thaliana and Shows an Endopathogenic Lifestyle
Salmonella enterica serovar typhimurium contaminated vegetables and fruits are considerable sources of human infections. Bacteria present in raw plant-derived nutrients cause salmonellosis, the world wide most spread food poisoning. This facultative endopathogen enters and replicates in host cells and actively suppresses host immune responses. Although Salmonella survives on plants, the underlying bacterial infection mechanisms are only poorly understood. In this report we investigated the possibility to use Arabidopsis thaliana as a genetically tractable host system to study Salmonella-plant interactions. Using green fluorescent protein (GFP) marked bacteria, we show here that Salmonella can infect various Arabidopsis tissues and proliferate in intracelullar cellular compartments. Salmonella infection of Arabidopsis cells can occur via intact shoot or root tissues resulting in wilting, chlorosis and eventually death of the infected organs. Arabidopsis reacts to Salmonella by inducing the activation of mitogen-activated protein kinase (MAPK) cascades and enhanced expression of pathogenesis related (PR) genes. The induction of defense responses fails in plants that are compromised in ethylene or jasmonic acid signaling or in the MKK3-MPK6 MAPK pathway. These findings demonstrate that Arabidopsis represents a true host system for Salmonella, offering unique possibilities to study the interaction of this human pathogen with plants at the molecular level for developing novel drug targets and addressing current safety issues in human nutrition
Prognostic factors and effect modifiers for personalisation of internet-based cognitive behavioural therapy among university students with subthreshold depression: A secondary analysis of a factorial trial
BACKGROUND: Internet-cognitive behavioural therapy (iCBT) for depression can include multiple components. This study explored depressive symptom improvement prognostic factors (PFs) and effect modifiers (EMs) for five common iCBT components including behavioural activation, cognitive restructuring, problem solving, self-monitoring, and assertion training. METHODS: We used data from a factorial trial of iCBT for subthreshold depression among Japanese university students (N = 1093). The primary outcome was the change in PHQ-9 scores at 8 weeks from baseline. Interactions between each component and various baseline characteristics were estimated using a mixed-effects model for repeated measures. We calculated multiplicity-adjusted p-values at 5 % false discovery rate using the Benjamini-Hochberg procedure. RESULTS: After multiplicity adjustment, the baseline PHQ-9 total score emerged as a PF and exercise habits as an EM for self-monitoring (adjusted p-values <0.05). The higher the PHQ-9 total score at baseline (range: 5-14), the greater the decrease after 8 weeks. For each 5-point increase at baseline, the change from baseline to 8 weeks was bigger by 2.8 points. The more frequent the exercise habits (range: 0-2 points), the less effective the self-monitoring component. The difference in PHQ-9 change scores between presence or absence of self-monitoring was smaller by 0.94 points when the participant exercised one level more frequently. Additionally, the study suggested seven out of 36 PFs and 14 out of 160 EMs examined were candidates for future research. LIMITATIONS: Generalizability is limited to university students with subthreshold depression. CONCLUSIONS: These results provide some helpful information for the future development of individualized iCBT algorithms for depression
The Asymmetry Coefficient for Interstellar Scintillation of Extragalactic Radio Sources
Comparing the asymmetry coefficients and scintillation indices for observed
time variations of the intensity of the radiation of extragalactic sources and
the predictions of theoretical models is a good test of the nature of the
observed variations. Such comparisons can be used to determine whether
flux-density variations are due to scintillation in the interstellar medium or
are intrinsic to the source. In the former case, they can be used to estimate
the fraction of the total flux contributed by the compact component (core)
whose flux-density variations are brought about by inhomogeneities in the
interstellar plasma. Results for the radio sources PKS 0405-385, B0917+624, PKS
1257-336, and J1819+3845 demonstrate that the scintillating component in these
objects makes up from 50% to 100% of the total flux, and that the intrinsic
angular sizes of the sources at 5 GHz is 10-40 microarcseconds. The
characteristics of the medium giving rise to the scintillations are presented
- …