Effects of a trapped vortex cell on thick wing profile

Experimental investigation on the effects originated from a trapped vortex cell on the NACA0024 airfoi

Sum-of-Squares approach to feedback control of laminar wake flows

A novel nonlinear feedback control design methodology for incompressible fluid flows aiming at the optimisation of long-time averages of flow quantities is presented. It applies to reduced-order finite-dimensional models of fluid flows, expressed as a set of first-order nonlinear ordinary differential equations with the right-hand side being a polynomial function in the state variables and in the controls. The key idea, first discussed in Chernyshenko et al. 2014, Philos. T. Roy. Soc. 372(2020), is that the difficulties of treating and optimising long-time averages of a cost are relaxed by using the upper/lower bounds of such averages as the objective function. In this setting, control design reduces to finding a feedback controller that optimises the bound, subject to a polynomial inequality constraint involving the cost function, the nonlinear system, the controller itself and a tunable polynomial function. A numerically tractable approach to the solution of such optimisation problems, based on Sum-of-Squares techniques and semidefinite programming, is proposed. To showcase the methodology, the mitigation of the fluctuation kinetic energy in the unsteady wake behind a circular cylinder in the laminar regime at Re=100, via controlled angular motions of the surface, is numerically investigated. A compact reduced-order model that resolves the long-term behaviour of the fluid flow and the effects of actuation, is derived using Proper Orthogonal Decomposition and Galerkin projection. In a full-information setting, feedback controllers are then designed to reduce the long-time average of the kinetic energy associated with the limit cycle. These controllers are then implemented in direct numerical simulations of the actuated flow. Control performance, energy efficiency, and physical control mechanisms identified are analysed. Key elements, implications and future work are discussed

Consumer acceptance of Italian or New Zealander lamb meat: an Italian case study

A Central location test was performed to compare heavy Apennine lamb meat to New Zealander lamb meat in order to create a Protected Geographical Indication for lamb meat. Roasted New-Zealander and heavy Apennine lamb legs were tested by 106 consumers according to the following experimental plan: blind phase (B) without any possibility to recognize the meat type tasted, expected phase (E) in which were described the characteristics of the two types of meat without tasting, informed phase (I) in which the tasted meat was recognized in provenience. Results showed in a nine point scale of appreciation for flavour, juiciness and overall pleasure, the highest values in Apennine lamb meat. B test showed the highest value for overall pleasure (P<0.01). Interaction, Italian lamb x Informed test showed the highest values for all the parameters except for overall liking for which Italian lamb x E test showed the highest values. About foreign lamb meat B test showed higher values than I and E test. Information about lamb meat origin showed disconfirmation for tenderness in Italian lamb meat. Foreign lamb meat showed a positive disconfirmation for flavour and overall pleasure, that confirm the better perception by consumers in blind consumption than in the informed one

Multi-time delay, multi-point Linear Stochastic Estimation of a cavity shear layer velocity from wall-pressure measurements

Multi-time-delay Linear Stochastic Estimation (MTD-LSE) technique is thoroughly described, focusing on its fundamental properties and potentialities. In the multi-time-delay ap- proach, the estimate of the temporal evolution of the velocity at a given location in the flow field is obtained from multiple past samples of the unconditional sources. The technique is applied to estimate the velocity in a cavity shear layer flow, based on wall-pressure measurements from multiple sensor

Groundwater resources in the main ethiopian rift valley: An overview for a sustainable development

In arid and semi-arid areas, human health and economic development depend on water availability, which can be greatly compromised by droughts. In some cases, the presence of natural contaminants may additionally reduce the availability of good quality water. This research analyzed the water resources and hydrochemical characteristics in a rural area of the central Main Ethiopian Rift Valley, particularly in the districts of Shashemene, Arsi Negelle, and Siraro. The study was developed using a census of the main water points (springs and wells) in the area and the sampling and physico-chemical analysis of the water, with particular regard to the fluoride concentration. In many cases, fluoride content exceeded the drinking water limits set by the World Health Organization, even in the absence of anthropogenic contamination. Two different aquifers were recognized: A shallow aquifer related to the eastern escarpment and highlands, and a deep aquifer in the lowland areas of the rift valley on the basis of compositional changes from Ca&ndash;Mg/HCO3 to Na&ndash;HCO3. The distribution of fluoride, as well as pH and EC values, showed a decrease from the center of the lowlands to the eastern highlands, with similar values closely aligned along an NNE/SSW trend. All these data contribute to creating awareness among and sharing information on the risks with rural communities and local governments to support the adequate use of the available water resources and to plan appropriate interventions to increase access to fresh water, aimed at the sustainable human and rural local development of the region

Genetic diversity in four populations of Nguni (Zulu) sheep assessed by microsatellite analysis

Zulu sheep are found mainly in the rural KwaZulu-Natal province and the numbers are declining due to indiscriminate inbreeding. There is thus a need for phenotypic and genetic characterisation as a first phase for planning conservation strategies. Zulu sheep populations sampled were from Makhathini research station (MS) (n=33), University of Zululand (UZ) (n=21), a community at KwaMthethwa (KM) (n=32) and from Msinga (EM) (n=33). One European breed Appen - ninica (AP) was used as out group. Microsatellite analysis using 29 microsatellite loci was used in this study. Among the Zulu sheep, the mean number of alleles per locus was the lowest (3.86) in UZ and the highest (6.24) was realised in EM. The mean values of observed and expected heterozygosity were 0.57 and 0.61, respectively. Neighbour-joining tree showed two main Zulu sheep clusters: the UZ, KM and MS sheep populations clustered together and the second cluster included only representatives from the EM population. The STRUCTURE analysis showed that KM, AP and EM were founded in separate clusters, whereas UZ and MS clustered together. The study demonstrated that there was a common origin of the population from the research stations (MS and UZ populations). It also demonstrated that the EM had a different history for the other three populations. This work suggests that exchange of rams could be useful in reducing inbreeding when considering conservation breeding programmes

Peptide Sequence and Conformation Strongly Influence Tryptophan Fluorescence

AbstractThis article probes the denatured state ensemble of ribonuclease Sa (RNase Sa) using fluorescence. To interpret the results obtained with RNase Sa, it is essential that we gain a better understanding of the fluorescence properties of tryptophan (Trp) in peptides. We describe studies of N-acetyl-L-tryptophanamide (NATA), a tripeptide: AWA, and six pentapeptides: AAWAA, WVSGT, GYWHE, HEWTV, EAWQE, and DYWTG. The latter five peptides have the same sequence as those surrounding the Trp residues studied in RNase Sa. The fluorescence emission spectra, the fluorescence lifetimes, and the fluorescence quenching by acrylamide and iodide were measured in concentrated solutions of urea and guanidine hydrochloride. Excited-state electron transfer from the indole ring of Trp to the carbonyl groups of peptide bonds is thought to be the most important mechanism for intramolecular quenching of Trp fluorescence. We find the maximum fluorescence intensities vary from 49,000 for NATA with two carbonyls, to 24,400 for AWA with four carbonyls, to 28,500 for AAWAA with six carbonyls. This suggests that the four carbonyls of AWA are better able to quench Trp fluorescence than the six carbonyls of AAWAA, and this must reflect a difference in the conformations of the peptides. For the pentapeptides, EAWQE has a fluorescence intensity that is more than 50% greater than DYWTG, showing that the amino acid sequence influences the fluorescence intensity either directly through side-chain quenching and/or indirectly through an influence on the conformational ensemble of the peptides. Our results show that peptides are generally better models for the Trp residues in proteins than NATA. Finally, our results emphasize that we have much to learn about Trp fluorescence even in simple compounds

Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice

<p>Abstract</p> <p>Background</p> <p>The correlation between neurofibrillary tangles of tau and disease progression in the brains of Alzheimer's disease (AD) patients remains an area of contention. Innovative data are emerging from biochemical, cell-based and transgenic mouse studies that suggest that tau oligomers, a pre-filament form of tau, may be the most toxic and pathologically significant tau aggregate.</p> <p>Results</p> <p>Here we report that oligomers of recombinant full-length human tau protein are neurotoxic in vivo after subcortical stereotaxic injection into mice. Tau oligomers impaired memory consolidation, whereas tau fibrils and monomers did not. Additionally, tau oligomers induced synaptic dysfunction by reducing the levels of synaptic vesicle-associated proteins synaptophysin and septin-11. Tau oligomers produced mitochondrial dysfunction by decreasing the levels of NADH-ubiquinone oxidoreductase (electron transport chain complex I), and activated caspase-9, which is related to the apoptotic mitochondrial pathway.</p> <p>Conclusions</p> <p>This study identifies tau oligomers as an acutely toxic tau species in vivo, and suggests that tau oligomers induce neurodegeneration by affecting mitochondrial and synaptic function, both of which are early hallmarks in AD and other tauopathies. These results open new avenues for neuroprotective intervention strategies of tauopathies by targeting tau oligomers.</p

Evaluating economic sustainability of the first automatic system for paddy irrigation in Europe

Italy is the leading rice producer in Europe, accounting for more than half of the total high-quality production of this crop. Rice is traditionally grown in fields that remain flooded starting with crop establishment until close to harvest, and this traditional irrigation technique (i.e., continuous submergence) is recognised as an important water resource sink (almost 40% of the irrigation water available worldwide is used for paddy areas). Meanwhile, the water management in rice areas requires a high level of labour because it is based on maintaining a predetermined water height in paddy fields and because the regulation of input and output flow is typically operated manually by the farmer. This study aims to evaluate the economical sustainability of adopting automatic and remote-controlled systems for irrigation of paddy fields. The results show that the investment is affordable for Italian farmers, it amount on average at about 650 \u20ac hectare-1 and it is repaid in about 15 year

High-density single nucleotide polymorphism markers reveal the population structure of 2 local chicken genetic resources

Italy counts a large number of local chicken populations, some without a recognized genetic structure, such as Val Platani (VPL) and Cornuta (COS), which represent noteworthy local genetic resources. In this study, the genotype data of 34 COS and 42 VPL, obtained with the Affymetrix Axiom600KChicken Genotyping Array, were used with the aim to investigate the genetic diversity, the runs of homozygosity (ROH) pattern, as well as the population structure and relationship within the framework of other local Italian and commercial chickens. The genetic diversity indices, estimated using different approaches, displayed moderate levels of genetic diversity in both populations. The identified ROH hotspots harbored genes related to immune response and adaptation to local hot temperatures. The results on genetic relationship and population structure reported a clear clustering of the populations according to their geographic origin. The COS formed a nonoverlapping genomic cluster and clearly separated from the other populations, but showed evident proximity to the Siciliana breed (SIC). The VPL highlighted intermediate relationships between the COS-SIC group and the rest of the sample, but closer to the other Italian local chickens. Moreover, VPL showed a complex genomic structure, highlighting the presence of 2 subpopulations that match with the different source of the samples. The results obtained from the survey on genetic differentiation underline the hypothesis that Cornuta is a population with a defined genetic structure. The substructure that characterizes the Val Platani chicken is probably the consequence of the combined effects of genetic drift, small population size, reproductive isolation, and inbreeding. These findings contribute to the understanding of genetic diversity and population structure, and represent a starting point for designing programs to monitor and safeguard these local genetic resources, in order to define a possible official recognition program as breeds