Analysis of the runoff response of an Alpine catchment at different scales

International audienceTo understand how hydrological processes are related across different spatial scales, 201 rainfall runoff events were examined in three nested catchments of the upper river Saalach in the Austrian Alps. The Saalach basin is a nested catchment covering different spatial scales, from the micro-scale (Limberg, 0.07 km2), to the small-catchment scale (Rammern, 15.5 km2), and the meso-scale (Viehhofen, 150 km2). At these three scales two different event types could clearly be identified, depending on rainfall characteristics and initial baseflow level: (1) a unimodal event type with a quick rising and falling hydrograph, responding to short duration rainfall, and (2) a bimodal event type with a double peak hydrograph at the micro-scale and substantially increased flow values at the larger basins Rammern and Viehhofen, responding to long duration rainfall events. In all cases where a bimodal event was identified at the microscale, the hydrographs at the larger scales exhibited significantly attenuated recession behavior, quantified by recession constants. At all scales, the bimodal events are associated with considerably higher runoff volumes than the unimodal events. From the investigations at the headwater Limberg we came to the conclusion that the higher amount of runoff of bimodal events is due to the mobilization of subsurface flow processes. The analysis shows that the occurrence of the two event types is consistent over three orders of magnitude in area. This link between the scales means that the runoff behavior of the headwater may be used as an indicator of the runoff behavior of much larger areas

Analysis of the runoff response of an alpine catchment at different scales

International audienceTo understand how hydrological processes are related across different spatial scales, 201 rainfall runoff events were examined in three nested catchments of the upper river Saalach in the Austrian Alps. The Saalach basin is a nested catchment covering different spatial scales, from the micro-scale (Limberg, 0.07 km²), to the small-catchment scale (Rammern, 15.5 km²), and the meso-scale (Viehhofen, 150 km²). At these three scales two different event types could clearly be identified, depending on rainfall characteristics and initial baseflow level: (1) a unimodal event type with a quick rising and falling hydrograph, responding to short duration rainfall, and (2) a bimodal event type with a double peak hydrograph at the micro-scale and substantially increased flow values at the larger basins Rammern and Viehhofen, responding to long duration rainfall events. In all cases where a bimodal event was identified at the microscale, the hydrographs at the larger scales exhibited significantly attenuated recession behavior, quantified by recession constants. At all scales, the bimodal events are associated with considerably higher runoff volumes than the unimodal events. From the investigations at the headwater Limberg we came to the conclusion that the higher amount of runoff of bimodal events is due to the mobilization of subsurface flow processes. The analysis shows that the occurrence of the two event types is consistent over three orders of magnitude in area. This link between the scales means that the runoff behavior of the headwater may be used as an indicator of the runoff behavior of much larger areas

Impact of snow state variation for design flood simulations in glacierized catchments

A continuously running hydrological energy balance model was used to analyse the influence of different snow covers on the formation of large floods within glacierized catchments. Model outputs from three different catchments in the Austrian Alps with glacierizations ranging from 30 to 39% were statistically interpreted to cover a wide range of possible combinations of areal snow cover distribution and mean snow water equivalent (SWE_m) and to define initialisation values for extreme runoff scenarios. These model states consider snow conditions between less than 10% snow coverage up to almost complete snow coverage, with different snow depths represented by minimum, mean or maximum levels of SWE_m. For the utilization of extreme runoffs, these snow cover states were combined with design storm input data. An analysis of the resulting flood hydrographs showed that the maximum runoff values occur with minimal snow cover conditions. With increasing snow coverage and SWE_m, flow magnitudes tend to decrease while the relevant precipitation time increases significantly. The influence of topographical catchment features, snow cover and SWE_m on the simulated scenario discharge is discussed. The contribution of the different runoff components to the modelled peak flows confirms the assumption that floods in glacierized catchments are a combination of directly discharging rain and ice melt

The value of multiple data set calibration versus model complexity for improving the performance of hydrological models in mountain catchments

The assessment of snow, glacier, and rainfall runoff contribution to discharge in mountain streams is of major importance for an adequate water resource management. Such contributions can be estimated via hydrological models, provided that the modeling adequately accounts for snow and glacier melt, as well as rainfall runoff. We present a multiple data set calibration approach to estimate runoff composition using hydrological models with three levels of complexity. For this purpose, the code of the conceptual runoff model HBV-light was enhanced to allow calibration and validation of simulations against glacier mass balances, satellite-derived snow cover area and measured discharge. Three levels of complexity of the model were applied to glacierized catchments in Switzerland, ranging from 39 to 103 km2. The results indicate that all three observational data sets are reproduced adequately by the model, allowing an accurate estimation of the runoff composition in the three mountain streams. However, calibration against only runoff leads to unrealistic snow and glacier melt rates. Based on these results, we recommend using all three observational data sets in order to constrain model parameters and compute snow, glacier, and rain contributions. Finally, based on the comparison of model performance of different complexities, we postulate that the availability and use of different data sets to calibrate hydrological models might be more important than model complexity to achieve realistic estimations of runoff composition

Hydrological modelling of glacierized catchments focussing on the validation of simulated snow patterns – applications within the flood forecasting system of the Tyrolean river Inn

The catchment of the river Inn is located in the Swiss and Austrian Alps. In the frame of the flood forecasting system "HoPI" (Hochwasserprognose für den Tiroler Inn), the Austrian part of the river Inn and its tributaries are covered within a hybrid numerical model. The runoff from the glacierized headwaters of the south-western Inn tributaries is calculated using the Snow- and Icemelt Model "SES" which utilizes a spatially-distributed energy balance approach; within SES, the accumulation and melting processes for snow, firn, and ice are considered. It is of great importance that such a type of model is used in the simulation of alpine areas since in these regions stream flow is influenced by the accumulation and melt of snow and ice and snow-free glaciers have also the potential to increase or even induce flood flow. For a prototype of the forecast system, SES was calibrated using the snow depletion of a glacier, but later, following the first results during the operational mode, the model was recalibrated and validated using remotely-sensed data covering all 13 glacierized catchments. Using the final snow-parameter setting, a simulation run of 15 hydrological years without any state corrections achieved overall agreements between observed and simulated snow cover ranging from 68% to 88% for all individual catchments. Runoff was calibrated and validated using the data from three different gauges. A parameter set, including both validated snow and runoff parameters, was applied for the modelling of a fourth gauged catchment and also achieved accurate results. This final unique parameterization was transferred to the remaining, ungauged watersheds

Prophylactic HPV vaccines: prospects for eliminating ano-genital cancer

Virtually all cases of cervical cancer and its precursor intra-epithelial lesions are a result of infection with one or other of a subset of genital human papillomaviruses (HPVs), suggesting that prevention of HPV infection by prophylactic vaccination would be a highly effective anticancer strategy. Two HPV L1 virus-like particle vaccines have been developed, a quadrivalent HPV16/18/6/11 product and a bivalent HPV16/18 product; both have been shown to be highly immunogenic with a good safety profile and 100% efficacy against HPV16/18-related high-grade cervical intra-epithelial neoplasia (CIN2/3), implying that they will be effective at preventing HPV16/18-related cervical cancer

Papillomavirus Capsid Binding and Uptake by Cells From Different Tissues and Species

The inability of papillomaviruses (PV) to replicate in tissue culture cells has hampered the study of the PV life cycle. We investigated virus-cell interactions by the following two methods: (i) using purified bovine PV virions or human PV type 11 (HPV type 11) virus-like particles (VLP) to test the binding to eukaryotic cells and (ii) using different VLP-reporter plasmid complexes of HPV6b, HPV11 L1 or HPV11 L1/L2, and HPV16 L1 or HPV16 L1/L2 to study uptake of particles into different cell lines. Our studies showed that PV capsids bind to a broad range of cells in culture in a dose-dependent manner. Binding of PV capsids to cells can be blocked by pretreating the cells with the protease trypsin. Penetration of PV into cells was monitored by using complexes in which the purified PV capsids were physically linked to DNA containing the gene for beta-galactosidase driven by the human cytomegalovirus promoter. Expression of beta-galactosidase occurred in < 1% of the cells, and the efficiency of PV receptor-mediated gene delivery was greatly enhanced (up to 10 to 20% positive cells) by the use of a replication-defective adenovirus which promotes endosomal lysis. The data generated by this approach further confirmed the results obtained from the binding assays, showing that PV enter a wide range of cells and that these cells have all functions required for the uptake of PV. Binding and uptake of PV particles can be blocked by PV-specific antisera, and different PV particles compete for particle uptake. Our results suggest that the PV receptor is a conserved cell surface molecule(s) used by different PV and that the tropism of infection by different PV is controlled by events downstream of the initial binding and uptake

A Pan-HPV Vaccine Based on Bacteriophage PP7 VLPs Displaying Broadly Cross-Neutralizing Epitopes from the HPV Minor Capsid Protein, L2

Current human papillomavirus (HPV) vaccines that are based on virus-like particles (VLPs) of the major capsid protein L1 largely elicit HPV type-specific antibody responses. In contrast, immunization with the HPV minor capsid protein L2 elicits antibodies that are broadly cross-neutralizing, suggesting that a vaccine targeting L2 could provide more comprehensive protection against infection by diverse HPV types. However, L2-based immunogens typically elicit much lower neutralizing antibody titers than L1 VLPs. We previously showed that a conserved broadly neutralizing epitope near the N-terminus of L2 is highly immunogenic when displayed on the surface of VLPs derived from the bacteriophage PP7. Here, we report the development of a panel of PP7 VLP-based vaccines targeting L2 that protect mice from infection with carcinogenic and non-carcinogenic HPV types that infect the genital tract and skin.L2 peptides from eight different HPV types were displayed on the surface of PP7 bacteriophage VLPs. These recombinant L2 VLPs, both individually and in combination, elicited high-titer anti-L2 IgG serum antibodies. Immunized mice were protected from high dose infection with HPV pseudovirus (PsV) encapsidating a luciferase reporter. Mice immunized with 16L2 PP7 VLPs or 18L2 PP7 VLPs were nearly completely protected from both PsV16 and PsV18 challenge. Mice immunized with the mixture of eight L2 VLPs were strongly protected from genital challenge with PsVs representing eight diverse HPV types and cutaneous challenge with HPV5 PsV.VLP-display of a cross-neutralizing HPV L2 epitope is an effective approach for inducing high-titer protective neutralizing antibodies and is capable of offering protection from a spectrum of HPVs associated with cervical cancer as well as genital and cutaneous warts

Murine Polyomavirus Virus-Like Particles Carrying Full-Length Human PSA Protect BALB/c Mice from Outgrowth of a PSA Expressing Tumor

Virus-like particles (VLPs) consist of capsid proteins from viruses and have been shown to be usable as carriers of protein and peptide antigens for immune therapy. In this study, we have produced and assayed murine polyomavirus (MPyV) VLPs carrying the entire human Prostate Specific Antigen (PSA) (PSA-MPyVLPs) for their potential use for immune therapy in a mouse model system. BALB/c mice immunized with PSA-MPyVLPs were only marginally protected against outgrowth of a PSA-expressing tumor. To improve protection, PSA-MPyVLPs were co-injected with adjuvant CpG, either alone or loaded onto murine dendritic cells (DCs). Immunization with PSA-MPyVLPs loaded onto DCs in the presence of CpG was shown to efficiently protect mice from tumor outgrowth. In addition, cellular and humoral immune responses after immunization were examined. PSA-specific CD4+ and CD8+ cells were demonstrated, but no PSA-specific IgG antibodies. Vaccination with DCs loaded with PSA-MPyVLPs induced an eight-fold lower titre of anti-VLP antibodies than vaccination with PSA-MPyVLPs alone. In conclusion, immunization of BALB/c mice with PSA-MPyVLPs, loaded onto DCs and co-injected with CpG, induces an efficient PSA-specific tumor protective immune response, including both CD4+ and CD8+ cells with a low induction of anti-VLP antibodies

A prospective study of the relationship between prediagnostic Human Papillomavirus seropositivity and HPV DNA in subsequent cervical carcinomas

Several prospective studies with invasive carcinoma as endpoint have supported Human Papillomavirus as a cause of cervical carcinoma. However, the largest study used seroepidemiology and did not analyse presence of Human Papillomavirus DNA in the subsequent tumour. Linkage of serum bank registries and cancer registries had identified 196 women with a registered cervical carcinoma after donation of a serum sample. For the present study, biopsies for 127 cases could be located, verified to contain invasive carcinoma and be amplified by PCR. Three control women who had remained alive and without cervical carcinoma during an equal length of follow-up had been matched to each of the case women and tested for HPV antibodies. Presence of Human Papillomavirus DNA in the tumours was analysed by general primer and type specific PCR. HPV16-seropositive women had a relative risk of 4.4 (95% CI: 2.2–8.8) to develop cervical carcinoma carrying HPV16 DNA. By contrast, there was no excess risk for Human Papillomavirus 16-seropositive women to develop cervical carcinoma devoid of HPV16 DNA. Prediagnostic HPV16 seropositivity was strongly correlated with later HPV16 DNA positivity of the tumour (P<0.001) and prediagnostic HPV18 seropositivity correlated with HPV18 DNA in the tumour (P<0.03). The link between prediagnostic seropositivity and type of viral DNA in the cancer implies that the carcinogenic effect of infection with these viruses is dependent on persistent presence of type-specific viral DNA