Automatic Vehicle Number Plate Recognition for Vehicle Parking Management System

A license plate recognition (LPR) system is one type of intelligent transportation system (ITS). It is a type of technology in which the software enables computer system to read automatically the license number plate of vehicle from digital pictures. Reading automatically the number plate means converting the pixel information of digital image into the ASCII text of the number plate. This paper discuses a method for the vehicle number plate recognition from the image using mathematical morphological operations. The main objective is to use different morphological operations in such a way that the number plate of vehicle can be identified accurately. This is based on various operation such as image enhancement, morphological transformation, edge detection and extraction of number plate from vehicle image. After this segmentation is applied to recognize the characters present on number plate using template matching. This algorithm can recognize number plate quickly and accurately from the vehicles image. Keywords: ANPR, ITS, Image Enhancement, Edge Detection, Morphological Operation, Number Plate Extraction,  Template Matching

Antigenic determinants on chicken riboflavin carrier protein. A study with monoclonal antibodies

Monoclonal antibodies raised against chicken egg white riboflavin carrier protein were classified into seven categories each recognizing a distinct epitope. Of these, six were directed against conformation dependent epitopes and one to a sequential epitope. The roles of lysine residues and the post-translationally attached phosphate and oligosaccharide moieties in the antigenicity of riboflavin carrier protein recognized by the monoclonal antibodies were investigated. The binding region of three monoclonal antibodies could be located within the 87-219 amino acid sequence of the protein and one antibody among these recognized a sequence of 182-204 amino acid residues. All the monoclonal antibodies were able to recognize riboflavin carrier proteins present in the sera of pregnant rats, cows and humans indicating that the epitopes to which they are directed are conserved through evolution from chicken to the human

Biochemical and immunological aspects of riboflavin carrier protein

Riboflavin carrier protein which is obligatorily involved in yolk deposition of the vitamin in the chicken egg, is a unique glycophosphoprotein present in both the yolk and white compartments. The yolk and egg white proteins are products of a single estrogen-inducible gene expressed in the liver and the oviduct respectively of egg laying birds. Despite the fact that the carbohydrate composition of the yolk and white riboflavin carrier proteins differ presumably due to differential post-translational modification, the proteins are immunologically similar and have identical amino acid sequence (including a cluster of 8 phosphoser residues towards the C-terminus) except at the carboxy terminus where the yolk riboflavin carrier protein lacks 13 amino acids as a consequence of proteolytic cleavage during uptake by oocytes. The protein is highly conserved throughout evolution all the way to humans in terms of gross molecular characteristics such as molecular weight and isoelectric point, and in immunological properties, preferential affinity for free riboflavin and estrogen inducibility at the biosynthetic locusviz., liver. Obligatory involvement of the mammalian riboflavin carrier protein in transplacental flavin transport to subserve fetal vitamin nutrition during gestation is revealed by experiments using pregnant rodent or subhuman primate models wherein immunoneutralisation of endogenous maternal riboflavin carrier protein results in fetal wastage followed by pregnancy termination due to selective yet drastic curtailment of vitamin efflux into the fetoplacental unit. Using monoclonal antibodies to chicken riboflavin carrier protein, it could be shown that all the major epitopes of the avian riboflavin carrier protein are highly conserved throughout evolution although the relative affinities of some of the epitopes for different monoclonal antibodies have undergone progressive changes during evolution. Using these monoclonal antibodies, an attempt is being made to map the different epitopes on the riboflavin carrier protein molecule with a view to delineate the immunodominant regions of the vitamin carrier to understand its structure-immunogenicity relationship

Intracytoplasmic type A particles from mammary tumours and leukaemias of strain ICRC mice.

The ovarian-hormone-induced leukaemias of strain ICRC mice, with an abundance of intracytoplasmic type A particles in primary as well as transplanted lesions, were used to study morphological, biophysical, immunological and structural characteristics of type A particles. Mammary tumours of strains ICRC and C3H(Jax) were also used as sources for type A particles. The purified virions banded at the density of 1.20 g/ml in 12--60% linear sucrose-density gradient when subjected to spinning at 113,000 g for 4 h. The SDS-polyacrylamide-gel electrophoresis of type A particles from mammary tumours and leukaemias reproducibly resolved at least 8 polypeptides, 2 of these 54,000 and 24,000 dalton proteins, showing variable expression. Type A particles and B particles, despite the fact that each had a distinct polypeptide pattern, showed common antigens with different electrophoretic mobilities. Proteins of 24,000, 18,000 and 12,000 daltons from B particles were found to be antigenically related to those from type A particles. The bioassay studies carried out with purified A particles showed that 2/7 males of strain ICRC and 1/6 females of strain DBA-MTI developed leukaemias, as against none in the controls, when inoculated between the ages of 1-7 days. Spleen tumour and cervical tumour were seen in one female each of strain DBA-MTI

Impact of metal on the DNA photocleavage activity and cytotoxicity of ferrocenyl terpyridine 3d metal complexes

Ferrocenyl terpyridine 3d metal complexes and their analogues, viz. [M(Fc-tpy)2](ClO4)2 (1-4), [Zn(Ph-tpy)2](ClO4)2 (5) and [Zn(Fc-dpa)2]X2 (X = ClO4, 6; PF6, 6a), where M = Fe(II) in 1, Co(II) in 2, Cu(II) in 3 and Zn(II) in 4, Fc-tpy is 4'-ferrocenyl-2,2':6',2''-terpyridine, Ph-tpy is 4'-phenyl-2,2':6',2''-terpyridine and Fc-dpa is ferrocenyl-N,N-dipicolylmethanamine, are prepared and their DNA binding and photocleavage activity in visible light studied. Complexes 2, 4, 5 and 6a that are structurally characterized by X-ray crystallography show distorted octahedral geometry with the terpyridyl ligands binding to the metal in a meridional fashion, with Fc-dpa in 6a showing a facial binding mode. The Fc-tpy complexes display a charge transfer band in the visible region. The ferrocenyl (Fc) complexes show a quasi-reversible Fc+-Fc redox couple within 0.48 to 0.66 V vs. SCE in DMF-0.1 M TBAP. The DNA binding constants of the complexes are 104 M-1. Thermal denaturation and viscometric data suggest DNA surface binding through electrostatic interaction by the positively charged complexes. Barring the Cu(II) complex 3, the complexes do not show any chemical nuclease activity in the presence of glutathione. Complexes 1-4 exhibit significant plasmid DNA photocleavage activity in visible light via a photoredox pathway. Complex 5, without the Fc moiety, does not show any DNA photocleavage activity. The Zn(II) complex 4 shows a significant PDT effect in HeLa cancer cells giving an IC50 value of 7.5 µ M in visible light, while being less toxic in the dark (IC50 = 49 µ M)

SMART CAR PARKING SYSTEM FOR VEHICLES

In day to day life theres massive drawback of parking of cars in metro cities attributable to lack of parking areas. So now a days we are constructing new system to solve this problem named as multilevel automatic parking system for vehicles Robotic automotive parking system. That the project work is to develop a automotive parking system that permits 6 to 26 cars inside an area of 32.17 sq.m with security of fingerprint ID for licensed entry solely. This model is very helpful for development in numerous areas like automation i.e. PLC micro-controllers and automation https://journalnx.co

The Bivariate Rogers-Szeg\"{o} Polynomials

We present an operator approach to deriving Mehler's formula and the Rogers formula for the bivariate Rogers-Szeg\"{o} polynomials $h_n(x,y|q)$. The proof of Mehler's formula can be considered as a new approach to the nonsymmetric Poisson kernel formula for the continuous big $q$-Hermite polynomials $H_n(x;a|q)$ due to Askey, Rahman and Suslov. Mehler's formula for $h_n(x,y|q)$ involves a ${}_3\phi_2$ sum and the Rogers formula involves a ${}_2\phi_1$ sum. The proofs of these results are based on parameter augmentation with respect to the $q$-exponential operator and the homogeneous $q$-shift operator in two variables. By extending recent results on the Rogers-Szeg\"{o} polynomials $h_n(x|q)$ due to Hou, Lascoux and Mu, we obtain another Rogers-type formula for $h_n(x,y|q)$. Finally, we give a change of base formula for $H_n(x;a|q)$ which can be used to evaluate some integrals by using the Askey-Wilson integral.Comment: 16 pages, revised version, to appear in J. Phys. A: Math. Theo

End tidal CO2 level (PETCO2) during laparoscopic surgery: comparison between spinal anaesthesia and general anaesthesia

Background: Laparoscopy is a procedure which involves insufflations of the abdomen by a gas, so that endoscope can visualise intra abdominal content without being in direct contact with viscera or tissues. Its advantages are small incisions, less pain, less postoperative ileus, short hospital stay compared to traditional open method. Monitoring of end tidal carbon dioxide (PETCO2) and hemodynamics is very necessary during Laparoscopy surgery. This study is conducted to find out effects of CO2 insufflation on parameters like PETCO2, Mean arterial pulse pressure, SPO2 under spinal anaesthesia and general anaesthesia in ASA I and ASA II patients.Methods: The present study was conducted in the department of anaesthesiology from December 2014 to September 2015.This study was a prospective, randomized controlled, single blind. Each group consisted of 30 patients having Group A and Group B as patient undergoing laparoscopic surgery under Spinal anaesthesia and General anaesthesia respectively. Preoperatively patients in Group A (Spinal anaesthesia) given inj. Midazolam 0.3mg/kg IM 45 before surgery and Group B (General anaesthesia) inj. pentazocin 0.3mg/kg, inj. promethazine 0.5mg/kg, inj. Glycopyrrolate 0.004 mg/kg IM 45 before surgery. In operation theatre, intra operative pulseoximetre, ECG, SPO2, Heart rate (HR), Mean arterial pulse pressure and PETCO2 monitoring done. Amount of CO2 insufflated noted.Results: It was found from present study that in both group there was significant progressive rise in PETCO2 after CO2 insufflation, with peak at 30 min and thereafter plateau till the end of procedure (avg. duration 45-60 min). In group A i.e. laparoscopic surgery under spinal anaesthesia with (spontaneous respiration) the rise in PETCO2 was significant as compared to the group B i.e. laparoscopic surgery under general anaesthesia with controlled ventilation. The heart rate increased after CO2 insufflation in both the group, but it was significant in group A. The increase in SBP, DBP, MAP were less in group A as compared to group B. SPO2 showed no significant changes and it remained above 97% in all patients throughout surgery. All values come to baseline 15 min after insufflation.Conclusions: From the present study it can be concluded that balanced general anaesthesia using IPPV with moderate hyperventilation, as the preferred anaesthetic technique for laparoscopic surgery

Immediate impact of an educational intervention on knowledge of use of disinfectants in nurses

Background: Adequate disinfection and sterilization is crucial to prevent hospital acquired infections, this requires knowledge of various types of disinfectants and of the categories of medical and surgical devices. Nurses play a key role in supervising the use of disinfectants. Hence, they are an important target group for educational interventions for rationalization of disinfectant use. We conducted an educational intervention in nurses, related to rational use of disinfectants. The objective was to evaluate the immediate impact of this intervention on change in knowledge of nurses.Methods: This was a questionnaire‑based pre‑ and post‑test cross‑sectional study. The questions were formulated to test nurses’ ability to (1) categorize commonly used medical and surgical devices (MSDs) (2) categorize disinfectants as high, intermediate and low level disinfectants (3) to evaluate their knowledge about different aspects of disinfectant use. Results of pre and post‑test were calculated as a percentage and Z test for difference between proportions was applied to test the statistical significance.Results: A total of 72 nurses filled the pre‑test and 70 the post‑test. Percentage of correct responders for classification of MSDs improved as follows‑critical (77.77% pre‑test to 95.71% post‑test), semicritical (18.05‑54.28%), noncritical (41.66‑72.85%). Percentage of correct responders for classification of disinfectants improved from pre‑ to post‑test glutaraldehyde (48.61‑88.57%), Hydrogen peroxide (30.55‑72.85%), benzalkonium (33.33‑58.57%). Identification of chlorine concentration required for cleaning floors improved from 38.88% to 70%. There was a significant improvement seen post‑test to items related to different aspects of disinfectant use.Conclusion: A positive immediate impact was observed, but there is a need for continuing education with interventions focused on various aspects of disinfectant use