Myrica esculenta Buch.-Ham. ex D. Don: A natural source for health promotion and disease preventdion

Myrica esculenta (Myricaceae) is a popular medicinal plant most commonly found in the sub-tropical Himalayas. It is widely used in folk medicine to treat several ailments such as asthma, cough, chronic bronchitis, ulcers, inflammation, anemia, fever, diarrhea, and ear, nose, and throat disorders. Due to its multidimensional pharmacological and therapeutic effects, it is well recognized in the ayurvedic pharmacopeia. However, the recent upsurge in M. esculenta use and demand has led to illicit harvesting by the horticultural trade and habitat loss, pushing the plant to the brink of extinction. Thus, the present review aims to provide updated information on M. esculenta botany, ethnomedicinal uses, phytochemistry, pharmacological effects, toxicity, and conservation methods, as well as also highlight prospective for future research. Particular emphasis is also given to its antioxidant potential in health promotion. In-depth literature was probed by searching several sources via online databases, texts, websites, and thesis. About 57 compounds were isolated and identified from M. esculenta, and the available reports on physicochemical parameters, nutritional and high-performance thin-layer chromatography analysis of bioactive plant parts are portrayed in a comparative manner. Friendly holistic conservation approaches offered by plant biotechnology applications, such as micropropagation, germplasm preservation, synthetic seed production, and hairy root technologies are also discussed. Nonetheless, further studies are needed to propose the mechanistic role of crude extracts and other bioactives, and even to explore the structure–function relationship of active components.Authors are thankful to A.P. Singh, Dean RIC, I. K. Gujral Punjab Technical University and members of sta_ in the department of RIC, I. K. Gujral Punjab Technical University for support and encouragement in this work. N. Martins would like to thank the Portuguese Foundation for Science and Technology (FCT-Portugal) for the Strategic project ref. UID/BIM/04293/2013 and “NORTE2020 - Northern Regional Operational Program” (NORTE-01-0145-FEDER-000012)

Phytochemical composition, antioxidant, and antimicrobial attributes of different solvent extracts from myrica esculenta buch.-ham. ex. d. don leaves

Background: Plant diversity is a basic source of food and medicine for local Himalayan communities. The current study was designed to assess the effect of different solvents (methanol, ethyl acetate, and water) on the phenolic profile, and the corresponding biological activity was studied. Methods: Antioxidant activity was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2¿-azino-bis(3-ethylbenzothiazoline-6-sulphonic) acid (ABTS) assay, while the antimicrobial activity was evaluated by disk diffusion method using various bacterial and fungal strains. Results: The outcomes demonstrated that methanol acted as the most effective solvent for polyphenols extraction, as strengthened by the liquid chromatography and mass spectroscopy (LC-MS) and fourier transform infrared spectroscopy (FTIR) analysis. M. esculenta methanol extract showed the highest DPPH and ABTS radical scavenger antioxidant activity with IC50 values of 39.29 µg/mL and 52.83 µg/mL, respectively, while the ethyl acetate and aqueous extracts revealed minimum antioxidant potential. Methanol extract also revealed higher phenolic content, 88.94 ± 0.24 mg of equivalent gallic acid (GAE)/g), measured by the Folin–Ciocalteu method, while the minimum content was recorded for aqueous extract (62.38 ± 0.14 GAE/g). The highest flavonoid content was observed for methanol extract, 67.44 ± 0.14 mg quercetin equivalent (QE)/g) measured by an aluminum chloride colorimetric method, while the lowest content was recorded for aqueous extract (35.77 ± 0.14 QE/g). Antimicrobial activity findings also reveal that the methanol extract led to a higher inhibition zone against bacterial and fungal strains. FTIR analysis reveals the presence of various functional groups, viz. alkenes, amines, carboxylic acids, amides, esters, alcohols, phenols, ketones, carboxylic acids, and aromatic compounds. This FTIR analysis could serve as a basis for the authentication of M. esculenta extracts for future industrial applications. Compounds identified by LC-MS analysis were gallic acid, myricanol, myricanone, epigallocatechin 3-O-gallate, ß-sitosterol, quercetin, p-coumaric acid, palmitic acid, n-pentadecanol, n-octadecanol, stigmasterol, oleanolic acid, n-hexadecanol, cis-ß-caryophyllene, lupeol, and myresculoside. Conclusion: This study suggests that the methanolic extract from M. esculenta leaves has strong antioxidant potential and could be a significant source of natural antioxidants and antimicrobials for functional foods formulation.Authors are thankful to A.P. Singh, Dean RIC, I.K. Gujral Punjab Technical University and members of staff in the department of RIC, I.K. Gujral Punjab Technical University for support and encouragement in this work. N. Martins would like to thank the Portuguese Foundation for Science and Technology (FCT-Portugal) for the Strategic project ref. UID/BIM/04293/2013 and “NORTE2020—Northern Regional Operational Program” (NORTE-01-0145-FEDER-000012)

Domain Private Transformers for Multi-Domain Dialog Systems

Large, general purpose language models have demonstrated impressive performance across many different conversational domains. While multi-domain language models achieve low overall perplexity, their outputs are not guaranteed to stay within the domain of a given input prompt. This paper proposes domain privacy as a novel way to quantify how likely a conditional language model will leak across domains. We also develop policy functions based on token-level domain classification, and propose an efficient fine-tuning method to improve the trained model's domain privacy. Experiments on membership inference attacks show that our proposed method has comparable resiliency to methods adapted from recent literature on differentially private language models.Comment: Accepted to Findings of EMNLP 2023 (short paper). Code available at https://github.com/asappresearch/domain-private-transformer

Studies in Sulphonamides Part VII: Synthesis of 1,3 diary1 2 (substituted sulphonamidobenzeneazo) propane 1, 3 diones and evaluation of their antibacterial properties

Different 1, 3-diarylpropane-1, 3-diones, 1-(P-nitropheny1) 3-phenyI-, p-nitropheny1) 3-(p-methyI-pheny1)- 1,)p-nitropheny1-3-(p-methoxypheny1)-, and 1-(m-nitropheny)-3- (p-methyIpheny1) propane 1, 3-diones have synthesised and coupled with a number a diazotised sulphonamide bases to yield the respective 1,3-diaryI-2-(substituted sulphonamidozeneazo) propane. All these substituted sulphonamidobenzence propane-1,3- diones have been screened in vitro for their antibacterial properties using cup plate agar diffusion method and some of these have been found active

Studies in Sulphonamides Part X

The present paper describes the synthesis of four differently substituted dibenzoylmethanes, viz., 1(m-nitropheny1)-3-(p-chloropheny1)-, 1-(m-notropheny1)-3-(p-bromopheny1)-, 1,3-di(p-methoxypheny1) and 1-(p-ethoxypheny1)-3-pheny1 propane-1,3-diones and study of their coupling reactions with different diazotised sulphonamide bases. The resulting 2-sulphonamidobenzeneazo derivatives were subjected to biological assay in vitro against S. aureus and E. coli and some of these found to posses considerable activity

Arrested States formed on Quenching Spin Chains with Competing Interactions and Conserved Dynamics

We study the effects of rapidly cooling to T = 0 a spin chain with conserved dynamics and competing interactions. Depending on the degree of competition, the system is found to get arrested in different kinds of metastable states. The most interesting of these has an inhomogeneous mixture of interspersed active and quiescent regions. In this state, the steady-state autocorrelation function decays as a stretched exponential $\sim \exp(-{(t/\tau_{o})}^{{1}\over{3}})$, and there is a two-step relaxation to equilibrium when the temperature is raised slightly.Comment: 4 pages, Latex, 3 postscript figures. Phys. Rev. E to appear (1999

Studies in Heterocyclic Compounds -Part VIII

Different azo compounds, 1-(m-nitrophenyl)-3-(p-bromophenyl)-and 1-(m-nitrophenyl) 3-(p-chlorophenyl)-2-(substituted sulphonamidobenzeneazo) propane-1,3-dinoes on condensation with hydrazine hydrate (100%), phenylhydrazine, p-nitrophenyllhydrazine and benzoylhydrazine yield the corresponding 1-simple/substituted-3-(m-nitrophenyl)-5-(p-bromo chlorophenyl)-4-(substituted sulphonamidobenzeneazo) pyrazoles. The homogeneity and purity of these was confirmed by TLC and these on screening in vitro against S. aureus and E. coli were found to exhibit antibacterial activity

Neutron diffraction analysis of stress and strain partitioning in a two-phase microstructure with parallel-aligned phases

By time-of-flight (TOF) neutron diffraction experiments, the influence of segregation-induced microstructure bands of austenite (γ) and martensite (α′ ) phases on the partitioning of stress and strain between these phases was investigated. Initially, tensile specimens of a Co-added stainless steel were heat treated by quenching and partitioning (Q&P) processing. Tensile specimens were subsequently loaded at 350 °C parallel to the length of the bands within the apparent elastic limit of the phase mixture. Lattice parameters in both axial and transverse directions were simultaneously measured for both phases. The observation of a lattice expansion for the γ phase in the transverse direction indicated a constraint on the free transverse straining of γ arising from the banded microstructure. The lateral contraction of α′ imposed an interphase tensile microstress in the transverse direction of the γ phase. The multiaxial stress state developed in the γ phase resulted in a large deviation from the level of plastic strain expected for uniaxial loading of single phase γ. Since segregation-induced banded microstructures commonly occur in many engineering alloys, the analysis of stress and strain partitioning with the present Q&P steel can be used to interpret the observations made for further engineering alloys with two-phase microstructures

Norspermidine is not a self-produced trigger for biofilm disassembly

SummaryFormation of Bacillus subtilis biofilms, consisting of cells encapsulated within an extracellular matrix of exopolysaccharide and protein, requires the polyamine spermidine. A recent study reported that (1) related polyamine norspermidine is synthesized by B. subtilis using the equivalent of the Vibrio cholerae biosynthetic pathway, (2) exogenous norspermidine at 25 μM prevents B. subtilis biofilm formation, (3) endogenous norspermidine is present in biofilms at 50–80 μM, and (4) norspermidine prevents biofilm formation by condensing biofilm exopolysaccharide. In contrast, we find that, at concentrations up to 200 μM, exogenous norspermidine promotes biofilm formation. We find that norspermidine is absent in wild-type B. subtilis biofilms at all stages, and higher concentrations of exogenous norspermidine eventually inhibit planktonic growth and biofilm formation in an exopolysaccharide-independent manner. Moreover, orthologs of the V. cholerae norspermidine biosynthetic pathway are absent from B. subtilis, confirming that norspermidine is not physiologically relevant to biofilm function in this species