Relationships between urina lithogenicity and neuro-humoral-immune factors in patients with chronic pyelonephrite and cholecystite

Hrinchenko B V, Snihur O V, Flyunt V R, Zukow W. Relationships between urina lithogenicity and neuro-humoral-immune factors in patients with chronic pyelonephrite and cholecystite. Journal of Education, Health and Sport. 2015;5(5):44-51. ISSN 2391-8306. DOI: 10.5281/zenodo.17369http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%285%29%3A44-51https://pbn.nauka.gov.pl/works/557141http://dx.doi.org/10.5281/zenodo.17369Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665X. Archives 2011 – 2014 http://journal.rsw.edu.pl/index.php/JHS/issue/archive Deklaracja.Specyfika i zawartość merytoryczna czasopisma nie ulega zmianie.Zgodnie z informacją MNiSW z dnia 2 czerwca 2014 r., że w roku 2014 nie będzie przeprowadzana ocena czasopism naukowych; czasopismo o zmienionym tytule otrzymuje tyle samo punktów co na wykazie czasopism naukowych z dnia 31 grudnia 2014 r.The journal has had 5 points in Ministry of Science and Higher Education of Poland parametric evaluation. Part B item 1089. (31.12.2014).© The Author (s) 2015;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland and Radom University in Radom, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 15.02.2015. Revised 27.03.2015. Accepted: 15.04.2015. RELATIONSHIPS BETWEEN URINA LITHOGENICITY AND NEURO-HUMORAL-IMMUNE FACTORS IN PATIENTS WITH CHRONIC PYELONEPHRITE AND CHOLECYSTITE B V Hrinchenko1, O V Snihur1, V R Flyunt1,2, W Zukow3 1JSC “Dnipro-Beskyd”, Truskavets’, Ukraine [email protected] of Balneology, OO Bohomolets’ Institute of Physiology, Truskavets’, Kyiv, Ukraine3Faculty of Physical Education, Health and Tourism, Kazimierz Wielki University, Bydgoszcz, Poland SUMMARYObjective. Despite a long history of research questions about the factors that determine lithogenicity urine remains open. Aim: to evaluate lithogenicity urine of patients with chronic pyelonephritis and cholecystitis and find her relationships with a concentration both in urine and plasma electrolytes and nitrogenous metabolites and the parameters gall-bladder motility, HRV, EEG and Immunity. Results. Lithogenicity urine Index (Lith) calculated by the formula: Lith=(Oxalates•Uric acid•Calcium/Magnesium•Creatinin)0,2, is determined positive by Oxalates (r=0,55) and Uric acid (r=0,34) but negative by Creatinin (r=-0,54) and Magnesium (r=-0,32). Revealed significantly correlation Lith with Uric acid Plasma level (r=-0,30) and Gall-bladder postprandial Volume, most 30 min (r=-0,51). Discovered unexpectedly negative correlation Lith with both Baevskiy Stress Index (r=-0,33) and Sympatho-Vagal Balance Index HRV (r=-0,36). Among EEG parameters correlated with Lith δ-rhythm amplitude (r=-0,50), θ-rhythm asymmetry index (r=0,45) and α-rhythm frequency (r=0,32). Among parameters of Immunity correlated with Lith blood levels of CD19+ B-Lymphocytes (r=0,34) and CD16+ NK-Lymphocytes (r=0,30). Canonical correlation between urina Lithogenicity and Neuro-Humoral-Immune factors is veri strong: R=0,97. Conclusion: in patients with chronic pyelonephritis and cholecystitis there are relationships between urina Lithogenicity and neuro-humoral-immune factors.Keywords: lithogenicity urine, neuro-humoral-immune factors, men

Relationships between caused by drinking of bioactive water Naftussya changes in urine lithogenicity and neuro-humoral-immune factors in humans with theirs abnormalities

Objective. Spa Truskavets' (Ukraine) considered to be indicated for the treatment and metaphylaxis renal stone disease. However, data on the effect of balneotherapy on the parameters of urine Lithogenicity ambiguous. This is due, perhaps, ambiguous influence balneotherapy on the neuroendocrine factors regulating exchange of electrolytes and uric acid. Aim: to find out the influence drinking of bioactive water Naftussya on urine lithogenicity and neuro-humoral-immune factors in humans with theirs abnormalities. Methods. The object of observation were ten women and ten men aged 33-76 years without clinical diagnose but with dysfunction of neuro-endocrine-immune complex and metabolism. In daily urine and blood we determined the content of electrolytes and nitrogenous metabolites, estimated parameters of immunity, recorded conductivity of acupuncture points and heart rate variability (HRV). After examination volunteers within 7 days used bioactive water Naftussya (250 ml three times a day), then repeated the tests listed. Results. Lithogenicity urine Index (Lith) calculated by formula: Lith=(Uric acid•Calcium/Magnesium•Creatinine)0,25. In 3 people initial normal Lith (0,65÷0,81 units) increased by 0,08÷0,16 units. In 4 people with normal or high Lith its changes were not detected (-0,01÷+0,01). In 12 people from a wide range of primary Lith (0,62÷1,07) it lowered by 0,03÷0,12 un. Even in a man maximum level of Lith (1,45 un.) down to the upper limit of normal (0,84 un.). Found a strong correlation between changes in Lith and a number parameters of HRV, metabolism and immunity. Conclusion. The use of bioactive water Naftussya causes ambiguous changes in Lithogenicity of urine, related to ambiguous changes in HRV, metabolism and immunity

Influence balneotherapy on spa Truskavets’ with taking Canephron® N on urina lithogenicity and its metabolic and neuroendocrine-immune accompaniments in patients with chronic pyelonephrite and cholecystite

Shulyak Oleksandr V., Flyunt Igor-Severyn S., Flyunt Victor R., Fil’ Vitaliǐ M., Hrinchenko Borys V., Snihur Oleh V., Zukow Walery, Yanchiy Oksana R., Popovych Igor L. Influence balneotherapy on spa Truskavets’ with taking Canephron® N on urina lithogenicity and its metabolic and neuroendocrine-immune accompaniments in patients with chronic pyelonephrite and cholecystite. Journal of Education, Health and Sport. 2015;5(11):453-465. ISSN 2391-8306. DOIhttp://dx.doi.org/10.5281/zenodo.34351http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%2811%29%3A453-465https://pbn.nauka.gov.pl/works/673673Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665X. Archives 2011–2014http://journal.rsw.edu.pl/index.php/JHS/issue/archive Deklaracja.Specyfika i zawartość merytoryczna czasopisma nie ulega zmianie.Zgodnie z informacją MNiSW z dnia 2 czerwca 2014 r., że w roku 2014 nie będzie przeprowadzana ocena czasopism naukowych; czasopismo o zmienionym tytule otrzymuje tyle samo punktów co na wykazie czasopism naukowych z dnia 31 grudnia 2014 r.The journal has had 5 points in Ministry of Science and Higher Education of Poland parametric evaluation. Part B item 1089. (31.12.2014).© The Author (s) 2015;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland and Radom University in Radom, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 25.09.2015. Revised 25.10.2015. Accepted: 24.11.2015. INFLUENCE BALNEOTHERAPY ON SPA TRUSKAVETS’ WITH TAKING CANEPHRON® N ON URINA LITHOGENICITY AND ITS METABOLIC AND NEUROENDOCRINE-IMMUNE ACCOMPANIMENTS IN PATIENTS WITH CHRONIC PYELONEPHRITE AND CHOLECYSTITE Oleksandr V. Shulyak1, Igor-Severyn S. Flyunt2, Victor R. Flyunt3,6, Vitaliǐ M. Fil’2,Borys V. Hrinchenko3, Oleh V. Snihur3, Walery Zukow4, Oksana R. Yanchiy5, Igor L. Popovych6 1Scientic Reseach Institute of Urology, Kyiv, Ukraine   [email protected] of Biology and Physical Education, Ivan Franko Pedagogical University, Drohobych, [email protected]    [email protected] “Dnipro-Beskyd”, Truskavets’, Ukraine   [email protected] of Physical Education, Health and Tourism, Kazimierz Wielki University, Bydgoszcz, Poland [email protected] of Biology, OO Bohomolets’ National Medical University, Kyiv, Ukraine6Laboratory of Balneology, OO Bohomolets’ Institute of Physiology NAS, Truskavets’-Kyiv, [email protected] SUMMARYObjective. We know that balneotherapy on spa Truskavets' did not significantly affect the parameters of urina Lithogenicity, including Ca/Mg ratio, so search for means of reducing urina Lithogenicity remains relevant. We also know that Phytopreparation Canephron® N recommended for inclusion in complex therapy of chronic pyelonephrite as effective elimination of infectious kidney inflammation, prevention of recurrence of inflammation and lithogenese. Aim: to find out the possibility of reducing urina Lithogenicity influenced balneotherapy on spa Truskavets' by additional taking Canephron® N. Results. Standardized balneotherapy not significantly affect urina Lithogenicity in patients with chronic pyelonephrite and cholecystite, even showed a trend to increase, while additional use Canephron® N significantly reduces urina Lithogenicity by reducing the concentration of Uric acid and downward trend Calcium. Reducing urina Lithogenicity accompanied by a decrease urina concentration Chloride and Potassium, and Baevskiy’s Activity Regulatory Systems Index, Asymmetry δ-, θ- and α-rhythms of background EEG and α-rhythm normalized Power Spectrum Density (PSD) in right loci Fp2, F8, T6 and O2, while HRV markers of vagal tone (RMSSD, pNN50, TI) also Frequency Deviation θ- and β-rhythms, Amplitude β- and δ-rhythms, δ-rhythm PSD in loci Fp2, F8, T3, T5, O1, O2, β-rhythm PSD in loci Fp1, F4 and θ-rhythm PSD in loci T3 and F7 rising. However, Canephron® N preventes or reverses increasing Uric acid plasma level, Mineralocorticoide Activity, Sympathetic tone, Baevskiy’s Stress Index and PSD ULF HRV also decreasing PSD VLF HRV. Concerning parameters of Immunity Canephron® N decreases blood level active T-Lymphocytes but increases level B-Lymphocytes, Stubnucleary Neutrophiles and total Leukocytes also prevents or reverses increasing CD16+ NK-Lymphocytes, CD8+ and Theophilinesensitive T-Lymphocytes, Monocytes and serum IgM also decreasing CD4+ T-Lymphocytes and Neutrophiles Phagocytose Count vs Staphylococcus aureus. In addition, Canephron® N potentiates increasing Parathyrine Activity, serum level IgG and Bactericidic Capacity of Neutrophiles vs Staphylococcus aureus also decreasing Urea and Creatinine plasma level, Sodium urina concentration and Neutrophiles Phagocytose Count vs Escherichia coli. Conclusion. Introduce Canephron® N into standard balneotherapeutic complex spa Truskavets’ contribute to decrease urina Lithogenicity accompanied by decreasing of HRV and EEG markers of chronic Stress and favorable modulation parameters of Immunity in patients with chronic pyelonephrite and cholecystite.Keywords: lithogenicity of urina, EEG, HRV, immunity, spa Truskavets’, Canephron® N

Similarity Of Effects On EEG Parameters Of Aramaic, Greek Catholic And Krishnaic Prayers

The neurotropic effects of Prayer are manifested in two inhibitory and three activating patterns. The first inhibitory pattern reflects the decrease in elevated and upper limit levels of SPD of θ-and δ-rhythm in frontal loci and the second - decrease in normal SPD levels of β-and θ-rhythm in the frontal, central, temporal and parietal loci. The first activating pattern reflects a small increase in normal levels of β-rhythm index and asymmetry and SPD entropy in locus C3, as well as a further increase in elevated δ-rhythm SPD levels in loci P3 and T3. The second pattern reflects the slight increase in normal SPD levels of θ-rhythm in loci T3, T5, T6, O2 and α-rhythm in locus T5, as well as their indices and entropy SPD in locus O2. The third pattern reflects a slight increase in amplitude and SPD of α-rhythm in central, frontal, temporal and occipital loci

The Interaction of Hypotaurine and Other Sulfinates with Reactive Oxygen and Nitrogen Species:A Survey of Reaction Mechanisms

Considerable strides have been made in understanding the oxidative mechanisms involved in the final steps of the cysteine pathway leading to taurine. The oxidation of sulfinates, hypotaurine and cysteine sulfinic acid, to the respective sulfonates, taurine and cysteic acid, has never been associated with any specific enzyme. Conversely, there is strong evidence that in vivo formation of taurine and cysteic acid is the result of sulfinate interaction with a variety of biologically relevant oxidants. In the last decade, many experiments have been performed to understand whether peroxynitrite, nitrogen dioxide and carbonate radical anion could be included in the biologically relevant reactive species capable of oxidizing sulfinates. Thanks to this work, it has been possible to highlight two possible reaction mechanisms (direct and indirect reaction) of sulfinates with reactive oxygen and nitrogen species.The sulfinates oxidation, mediated by peroxynitrite, is an example of both reaction mechanisms: through a two-electron-direct-reaction with peroxynitrite or through a one-electron-indirect-transfer reaction. In the indirect mechanism, the peroxynitrite homolysis releases hydroxyl and nitrogen dioxide radical and in addition the degradation of short-lived adduct formed by peroxynitrite and CO2 can generate carbonate radical anion. The reaction of hypotaurine and cysteine sulfinic acid with peroxynitrite-derived radicals is accompanied by extensive oxygen uptake with the generation of transient intermediates, which can begin a reaction by an oxygen-dependent mechanism with the sulfonates, taurine, and cysteic acid as final products. Due to pulse radiolysis studies, it has been shown that transient sulfonyl radicals (RSO2(•)) have been produced during the oxidation of both sulfinates by one-electron transfer reaction.The purpose is to analyze all the aspects of the reactive mechanism in the sulfinic group oxidation of hypotaurine and cysteine sulfinic acid through the results obtained from our laboratory in recent years

Some neural and hemato-immune factors correlated with lysozime saliva level in children living on territories polluted by radionucleides

Seniv T S, Seniv R A, Flyunt I S, Uhryn M R, Zukow W. Some neural and hemato-immune factors correlated with lysozime saliva level in children living on territories polluted by radionucleides. Journal of Education, Health and Sport. 2015;5(5):275-282. ISSN 2391-8306. DOI 10.5281/zenodo.17737 http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%285%29%3A275-282 https://pbn.nauka.gov.pl/works/560515 http://dx.doi.org/10.5281/zenodo.17737 Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665X. Archives 2011 – 2014 http://journal.rsw.edu.pl/index.php/JHS/issue/archive   Deklaracja. Specyfika i zawartość merytoryczna czasopisma nie ulega zmianie. Zgodnie z informacją MNiSW z dnia 2 czerwca 2014 r., że w roku 2014 nie będzie przeprowadzana ocena czasopism naukowych; czasopismo o zmienionym tytule otrzymuje tyle samo punktów co na wykazie czasopism naukowych z dnia 31 grudnia 2014 r. The journal has had 5 points in Ministry of Science and Higher Education of Poland parametric evaluation. Part B item 1089. (31.12.2014). © The Author (s) 2015; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland and Radom University in Radom, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 15.02.2015. Revised 27.04.2015. Accepted: 08.05.2015.   SOME NEURAL AND HEMATO-IMMUNE FACTORS CORRELATED WITH LYSOZIME SALIVA LEVEL IN CHILDREN LIVING ON TERRITORIES POLLUTED BY RADIONUCLEIDES   T S Seniv1, R A Seniv1, I S Flyunt2, M R Uhryn2, W Zukow3   1Clinical Sanatorium “Moldova”, Truskavets’, Ukraine [email protected] 2Faculty of Physical Education Ivan Franko University, Drohobych, Ukraine 3Faculty of Physical Education, Health and Tourism, Kazimierz Wielki University, Bydgoszcz, Poland   Summary Objective. We know that children who live on territories polluted by radionucleides, characterized, among other things, increased prevalence diseases to caries and periodontitis. On the other hand, for the rest of this contingent characterized by dysfunction of the neuroendocrine-immune complex. We set a goal to find out the relationships saliva lysozyme activity - a key factor in antibacterial protection of the oral cavity - with some blood-neural and immune factors. Methods. The study involved 80 children aged 10-17 years, who arrived to the spa Truskavets’ from territories polluted by radionucleides. In addition to the activity of lysozyme in saliva, recorded parameters of immune status I-II levels of WHO, HRV, Stange and Hench tests, and conducted routine clinical tests. Results. Normal activity of saliva lysozyme pronounced only in 17 (21%) children. Regarding immunity parameters detected significant positive correlation activity of lysozyme with the intensity (r=0,49) and activity (r=0,34) phagocytosis by neutrophils blood Staph. aureus and concentration of serum IgM (r=0,34) and circulating immune complexes (r=0,30), whereas a negative correlation with the levels of IgA (r=-0,46), CD19+ B lymphocytes (r=-0,32) theophilinsensitive T lymphocytes (r=-0,26) and CD8+ T lymphocytes (r=-0,23). Among the HRV parameters with the activity of lysozyme only weakly correlated markers vagal tone RMSSD (r=-0,19) i PSD HF (r=-0,19). The observed negative correlation lysozyme activity of inspiratory test Stange (r=-0,47) and expiratory test Hench (r=-0,27). Among hemogram parameters significantly correlated with the activity of lysozyme only platelet level (r=-0,22). The canonical correlation between saliva lysozyme activity and registered blood-neural and immune factors proved strong (R=0,82). Conclusion. Reduced activity of lysozyme in saliva of children, who live on territories polluted by radionucleides, is one of the manifestations of dysfunction neuroendocrine-immune complex.   Keywords: saliva lysozyme, immunity, HRV, Stange and Hench tests, children

Shedding light on the soft and efficient free radical induced reduction of graphene oxide: Hidden mechanisms and energetics

Reduction of graphene oxide (GO) in aqueous dispersions by strongly reducing free radicals has recently been identified to be a very powerful approach, because functional groups are removed softly but efficiently, and non-volatile impurities as well as defects are largely avoided. However, the reaction mechanisms remained somewhat speculative. Recently we showed that GO can be efficiently reduced in water by indirect photoreduction mediated by (CH3)2C(OH) radicals generated via the reaction of triplet acetone with isopropanol. Those radicals efficiently defunctionalize oxo-groups of GO forming the carbon lattice without generating additional defects. In this comprehensive study we shed more light on the reaction mechanism of reduction of GO by H, CO2 -, (CH3)2C(OH) and CH3CH(OH) by combining pulsed radiolysis and determine its overall energetics via quantum-chemical calculations. In time-dependent experiments mechanistic insights have been obtained and unknown intermediates have been discovered. Moreover, different reduction mechanisms, such as radical addition, electron-transfer, concerted water elimination and HCO3 - elimination are identified. Here we show that all mechanisms lead to sp2-carbon formation and therefore high quality graphene by reductive defunctionalization

High quality reduced graphene oxide flakes by fast kinetically controlled and clean indirect UV-induced radical reduction

This work highlights a surprisingly simple and kinetically controlled highly efficient indirect method for the production of high quality reduced graphene oxide (rGO) flakes via UV irradiation of aqueous dispersions of graphene oxide (GO), in which the GO is not excited directly. While the direct photoexcitation of aqueous GO (when GO is the only light-absorbing component) takes several hours of reaction time at ambient temperature (4 h) leading only to a partial GO reduction, the addition of small amounts of isopropanol and acetone (2% and 1%) leads to a dramatically shortened reaction time by more than two orders of magnitude (2 min) and a very efficient and soft reduction of graphene oxide. This method avoids the formation of non-volatile species and in turn contamination of the produced rGO and it is based on the highly efficient generation of reducing carbon centered isopropanol radicals via the reaction of triplet acetone with isopropanol. While the direct photolysis of GO dispersions easily leads to degradation of the carbon lattice of GO and thus to a relatively low electric conductivity of the films of flakes, our indirect photoreduction of GO instead largely avoids the formation of defects, keeping the carbon lattice intact. Mechanisms of the direct and indirect photoreduction of GO have been elucidated and compared. Raman spectroscopy, XPS and conductivity measurements prove the efficiency of the indirect photoreduction in comparison with the state-of-the-art reduction method for GO (hydriodic acid/trifluoroacetic acid). The rapid reduction times and water solvent containing only small amounts of isopropanol and acetone may allow easy process up-scaling for technical applications and low-energy consumption

Modulating effects of bioactive water Naftussya from layers Truskavets' and Pomyarky on some metabolic and biophysic parameters at humans with dysfunction of neuro-endocrine-immune complex

Gozhenko Anatoliy I, Sydoruk Nadiya O, Babelyuk Valeriy Ye, Dubkova Galyna I, Flyunt Viktor R, Hubyts’kyi Viktor Yo, Zukow Walery, Barylyak Liliya G, Popovych Igor L. Modulating effects of bioactive water Naftussya from layers Truskavets’ and Pomyarky on some metabolic and biophysic parameters at humans with dysfunction of neuro-endocrine-immune complex. Journal of Education, Health and Sport. 2016;6(12):826-842. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.238225 http://ojs.ukw.edu.pl/index.php/johs/article/view/4162 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 754 (09.12.2016). 754 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 05.12.2016. Revised 20.12.2016. Accepted: 25.12.2016. MODULATING EFFECTS OF BIOACTIVE WATER NAFTUSSYA FROM LAYERS TRUSKAVETS’ AND POMYARKY ON SOME METABOLIC AND BIOPHYSIC PARAMETERS AT HUMANS WITH DYSFUNCTION OF NEURO-ENDOCRINE-IMMUNE COMPLEX Anatoliy I Gozhenko1, Nadiya O Sydoruk1,2, Valeriy Ye Babelyuk3, Galyna I Dubkova3, Viktor R Flyunt2, Viktor Yo Hubyts’kyi3, Walery Zukow4, Liliya G Barylyak5, Igor L Popovych5 1Ukrainian Scientific Research Institute of Transport Medicine Ministry of Health, Odesa [email protected] 2JSC “Dnipro-Beskyd”, Truskavets’, Ukraine [email protected] [email protected] 3Clinical sanatorium „Моldova”, Truskavets’, Ukraine [email protected] 4Faculty of Physical Education, Health and Tourism, Kazimierz Wielki University, Bydgoszcz, Poland [email protected] 5Laboratory of Experimental Balneology, OO Bogomoletz Institute of Physiology NAS, Kyiv, Ukraine [email protected] Abstracts Background. Previously we have been carry out comparative investigation immediate effects of Bioactive Water Naftussya from layers Truskavets’, Pomyarky and Skhidnyts’a on neuro-endocrine-immune complex at men with its dysfunction. The aim of this study is the influence of the use of the course of Bioactive Water Naftussya from layers Truskavets’ and Pomyarky on some metabolic and biophysical parameters at similar patients. Materials and methods. The object of observation were 20 volunteers: ten women and ten men aged 33-76 years without clinical diagnose but with dysfunction of neuro-endocrine-immune complex and metabolism. In daily urine and venous blood we determined the content of electrolytes, nitrogenous metabolites and lipids, recorded conductivity of acupuncture points, rate of electronegative nuclei of buccal epithelium as well as parameters of gas discharge vizualisation (GDV). After examination volunteers within 7 days used bioactive water Naftussya (250 mL three times a day) from Truskavets’ or Pomyarky layer, then repeated the tests listed. Results. Weekly use of Bioactive Water Naftussya increases in the normal level of plasma chloride and sodium, normalizes low level of bicarbonate and decreases within the normal levels of potassium and phosphate. Urinary excretion of sodium and chloride increases while excretion and concentration of uric acid decreases, as the urine concentration of phosphates. The index lithogenicity urine decreased from 112% to 103% norm standard. Initially reduced level of plasma triacylglycerides increases, while decreases in the normal level of cholesterol in low-density lipoprotein composition. Among the biophysical parameters detected increase in the normal conductivity acupuncture points Pg (ND) at right side, which represent the nervous system, and left shift the ratio between the conductivity of acupuncture points MC (AVL), which represents the immune system. Increases electrokinetic index of epithelial cells of cheek to 1,7±0,3%, that indicating the "rejuvenation" of the organism to 1,4±0,3 years. We first discovered changes in parameters of GDV, namely elimination of asymmetries of virtual chakras (first, second and third) as well as reducing the excess energy of the first chakra. No significant differences between the effects of Bioactive Water Naftussya both fields generally not found. Conclusion. Bioactive water Naftussya both Truskavets’ and Pomyarky layers causes favorable normalizing effects on abnormalities parameters of metabolism and gas discharge visualization, which is a manifestation of its adaptogenic properties. Keywords: bioactive water Naftussya, electrolytes, nitrogenous metabolites, lipides, electroskin conductivity, gas discharge vizualisation