Ruminal Fill Effect of Forages: Prediction and Relationship with Voluntary Intake

Voluntary dry matter intake (VDMI) and rumen fill were measured on sheep fed with 18 forages ranging from wheat straw to lucerne hay. In vivo fill effect (IVFE i.e. rumen DM pool divided by VDMI), in situ degradability, cell-wall composition, pepsin-cellulase digestibility and in vitro gas production were determined. In situ estimated fill effect (ISFE) was calculated as the retention time of insoluble potential degradable and undegradable fractions using a constant rate of passage. ISFE and IVFE were highly correlated (r2=0.89) but ISFE values were lower than IVFE values because in situ degradability does not integrate comminution time of large particles. NDF content, pepsin-cellulase digestibility and gas production were good predictors of IVFE (r2=0.86, 0.90 and 0.91 respectively) and to a lesser extent of VDMI (r2=0.78, 0.88 and 0.84)

Differential Forms on Log Canonical Spaces

The present paper is concerned with differential forms on log canonical varieties. It is shown that any p-form defined on the smooth locus of a variety with canonical or klt singularities extends regularly to any resolution of singularities. In fact, a much more general theorem for log canonical pairs is established. The proof relies on vanishing theorems for log canonical varieties and on methods of the minimal model program. In addition, a theory of differential forms on dlt pairs is developed. It is shown that many of the fundamental theorems and techniques known for sheaves of logarithmic differentials on smooth varieties also hold in the dlt setting. Immediate applications include the existence of a pull-back map for reflexive differentials, generalisations of Bogomolov-Sommese type vanishing results, and a positive answer to the Lipman-Zariski conjecture for klt spaces.Comment: 72 pages, 6 figures. A shortened version of this paper has appeared in Publications math\'ematiques de l'IH\'ES. The final publication is available at http://www.springerlink.co

Molecular developmental evidence for a subcoxal origin of pleurites in insects and identity of the subcoxa in the gnathal appendages

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ The attached file is the published version of the article

Evolution of Game Controllers: Toward the Support of Gamers with Physical Disabilities

Video games, as an entertaining media, dates back to the \u201850s and their hardware device underwent a long evolution starting from hand-made devices such as the \u201ccathode-ray tube amusement device\u201d up to the modern mass-produced consoles. This evolution has, of course, been accompanied by increasingly specialized interaction mechanisms. As of today, interaction with games is usually performed through industry-standard devices. These devices can be either general purpose (e.g., mouse and keyboard) or specific for gaming (e.g., a gamepad). Unfortunately, for marketing reasons, gaming interaction devices are not usually designed taking into consideration the requirements of gamers with physical disabilities. In this paper, we will offer a review of the evolution of gaming control devices with a specific attention to their use by players with physical disabilities in the upper limbs. After discussing the functionalities introduced by controllers designed for disabled players we will also propose an innovative game controller device. The proposed game controller is built around a touch screen interface which can be configured based on the user needs and will be accessible by gamers which are missing fingers or are lacking control in hands movement

Over-expression of adenosine deaminase in mouse podocytes does not reverse puromycin aminonucleoside resistance

<p>Abstract</p> <p>Background</p> <p>Edema in nephrotic syndrome results from renal retention of sodium and alteration of the permeability properties of capillaries. Nephrotic syndrome induced by puromycin aminonucleoside (PAN) in rats reproduces the biological and clinical signs of the human disease, and has been widely used to identify the cellular mechanisms of sodium retention. Unfortunately, mice do not develop nephrotic syndrome in response to PAN, and we still lack a good mouse model of the disease in which the genetic tools necessary for further characterizing the pathophysiological pathway could be used. Mouse resistance to PAN has been attributed to a defect in glomerular adenosine deaminase (ADA), which metabolizes PAN. We therefore attempted to develop a mouse line sensitive to PAN through induction of normal adenosine metabolism in their podocytes.</p> <p>Methods</p> <p>A mouse line expressing functional ADA under the control of the podocyte-specific podocin promoter was generated by transgenesis. The effect of PAN on urinary excretion of sodium and proteins was compared in rats and in mice over-expressing ADA and in littermates.</p> <p>Results</p> <p>We confirmed that expression of ADA mRNAs was much lower in wild type mouse than in rat glomerulus. Transgenic mice expressed ADA specifically in the glomerulus, and their ADA activity was of the same order of magnitude as in rats. Nonetheless, ADA transgenic mice remained insensitive to PAN treatment in terms of both proteinuria and sodium retention.</p> <p>Conclusions</p> <p>Along with previous results, this study shows that adenosine deaminase is necessary but not sufficient to confer PAN sensitivity to podocytes. ADA transgenic mice could be used as a background strain for further transgenesis.</p

Transcriptional Responses of Arabidopsis thaliana during Wilt Disease Caused by the Soil-Borne Phytopathogenic Bacterium, Ralstonia solanacearum

Bacterial wilt is a common disease that causes severe yield and quality losses in many plants. In the present study, we used the model Ralstonia solanacearum-Arabidopsis thaliana pathosystem to study transcriptional changes associated with wilt disease development. Susceptible Col-5 plants and RRS1-R-containing resistant Nd-1 plants were root-inoculated with R. solanacearum strains harbouring or lacking the matching PopP2 avirulence gene. Gene expression was marginally affected in leaves during the early stages of infection. Major changes in transcript levels took place between 4 and 5 days after pathogen inoculation, at the onset of appearance of wilt symptoms. Up-regulated genes in diseased plants included ABA-, senescence- and basal resistance-associated genes. The influence of the plant genetic background on disease-associated gene expression is weak although some genes appeared to be specifically up-regulated in Nd-1 plants. Inactivation of some disease-associated genes led to alterations in the plant responses to a virulent strain of the pathogen. In contrast to other pathosystems, very little overlap in gene expression was detected between the early phases of the resistance response and the late stages of disease development. This observation may be explained by the fact that above-ground tissues were sampled for profiling whereas the bacteria were applied to root tissues