A study of some aspects of growth and reproduction in two inbred lines of mice and their crosses : a thesis presented in partial fulfilment of the requirements for the degree of Master of Agricultural Science in Massey University of Manawatu

The mating of closely related individuals is generally believed to result in inferior offspring. Recognition of this danger has been suggested as the reason for evolution of human social customs. However, historical researches suggest that inbreeding unavoidably occurred in small, isolated, ancient communities, and marriage of close relatives was encouraged in the Egyptian, Greek and Hebrew civilisations (Zirkle, 1952). Inbreeding is likely to have occurred in the isolated flocks of nomads, but it is probable that inbreeding depression, as such, was not recognised until the eighteenth century when the early livestock improvers are reputed to have found that the fertility of their animals deteriorated with continued inbreeding. Hybrid vigour from cross breeding plants was described by Koelreuter (1766) and confirmed by later botanists., .After much detailed work, Darwin (1876) concluded: " •••cross fertilisation is generally beneficial and self fertilisation injurious." and that (a) Mechanisms exist widely for the avoidance of inbreeding. (b) Inbreeding has effects likely to incur selective disadvantages. No suitable hypothesis could account for the usually deleterious effects of inbreeding, or the reverse phenomenon of hybrid vigour until the rediscovery of Mendel's work in 1900. The effects of inbreeding can now be explained in terms of the dominance and recessiveness of genes. (Falconer, 1960). The aim of experiments reported in this thesis was to study growth, reproduction and mortality in two inbred lines of mice, derived from common ancestors, to find if differences between them had arisen during inbreeding. Reciprocal crosses and matings of the F1 progeny of these crosses were made to find if hybridisation of the parent lines led to improvements of these charact­eristics. This study was suggested by Drs. D.S.. Flux, M.F. McDonald and R.E. Munford to whom the author is indebted for advice and guidance. The author also wishes to acknowledge the assistance of Mr. E. Ormsby in histological preparations, of Mr. P .S. Dale in photomicrography, and of Mr. C. Muir who drew some of the figures reproduced in this thesis. Thanks are also due to the staff of the University Library for their efforts in obtaining literature. [From Preface

Manifestation of three-body forces in f7/2-shell nuclei

The traditional nuclear shell model approach is extended to include many-body forces. The empirical Hamiltonian with a three-body force is constructed for the identical nucleons on the 0f7/2 shell. Manifestations of the three-body force in spectra, binding energies, seniority mixing, particle-hole symmetry, electromagnetic and particle transition rates are investigated. It is shown that in addition to the usual expansion of the valence space within the tranditional two-body shell model, the three-body component in the Hamiltonian can be an important part improving the quality of the theoretical approach.Comment: 5 pages, 1 figur

Localization of the Major NF-κB-activating Site and the Sole TRAF3 Binding Site of LMP-1 Defines Two Distinct Signaling Motifs

The TRAF3 molecule interacts with the cytoplasmic carboxyl terminus (COOH terminus) of the Epstein-Barr virus-encoded oncogene LMP-1. NF-κB activation is a downstream signaling event of tumor necrosis factor receptor-associated factor (TRAF) molecules in other signaling systems (CD40 for example) and is an event caused by LMP-1 expression. One region capable of TRAF3 interaction in LMP-1 is the membrane-proximal 45 amino acids (188–242) of the COOH terminus. We show that this region contains the only site for binding of TRAF3 in the 200-amino acid COOH terminus of LMP-1. The site also binds TRAF2 and TRAF5, but not TRAF6. TRAF3 binds to critical residues localized between amino acids 196 and 212 (HHDDSLPHPQQATDDSG), including the PXQX(T/S) motif, that share limited identity to the CD40 receptor TRAF binding site (TAAPVQETL). Mutation of critical residues in the TRAF3 binding site of LMP-1 that prevents binding of TRAF2, TRAF3, and TRAF5 does not affect NF-κB-activating potential. Deletion mapping localized the major NF-κB activating region of LMP-1 to critical residues in the distal 4 amino acids of the COOH terminus (383–386). Therefore, TRAF3 binding and NF-κB activation occur through two separate motifs at opposite ends of the LMP-1 COOH-terminal sequence

Hierarchical strategies for efficient fault recovery on the reconfigurable PAnDA device

A novel hierarchical fault-tolerance methodology for reconfigurable devices is presented. A bespoke multi-reconfigurable FPGA architecture, the programmable analogue and digital array (PAnDA), is introduced allowing fine-grained reconfiguration beyond any other FPGA architecture currently in existence. Fault blind circuit repair strategies, which require no specific information of the nature or location of faults, are developed, exploiting architectural features of PAnDA. Two fault recovery techniques, stochastic and deterministic strategies, are proposed and results of each, as well as a comparison of the two, are presented. Both approaches are based on creating algorithms performing fine-grained hierarchical partial reconfiguration on faulty circuits in order to repair them. While the stochastic approach provides insights into feasibility of the method, the deterministic approach aims to generate optimal repair strategies for generic faults induced into a specific circuit. It is shown that both techniques successfully repair the benchmark circuits used after random faults are induced in random circuit locations, and the deterministic strategies are shown to operate efficiently and effectively after optimisation for a specific use case. The methods are shown to be generally applicable to any circuit on PAnDA, and to be straightforwardly customisable for any FPGA fabric providing some regularity and symmetry in its structure

Young stars in Epsilon Cha and their disks: disk evolution in sparse associations

(abridge) The nearby young stellar association Epsilon Cha association has an estimated age of 3-5 Myr, making it an ideal laboratory to study the disk dissipation process and provide empirical constraints on the timescale of planet formation. We combine the available literature data with our Spitzer IRS spectroscopy and VLT/VISIR imaging data. The very low mass stars USNO-B120144.7 and 2MASS J12005517 show globally depleted spectral energy distributions pointing at strong dust settling. 2MASS J12014343 may have a disk with a very specific inclination where the central star is effectively screened by the cold outer parts of a flared disk but the 10 micron radiation of the warm inner disk can still reach us. We find the disks in sparse stellar associations are dissipated more slowly than those in denser (cluster) environments. We detect C_{2}H_{2} rovibrational band around 13.7 micron on the IRS spectrum of USNO-B120144.7. We find strong signatures of grain growth and crystallization in all Epsilon Cha members with 10 micron features detected in their IRS spectra. We combine the dust properties derived in the Epsilon Cha sample with those found using identical or similar methods in the MBM 12, Coronet cluster, Eta Cha associations, and in the cores to disks (c2d) legacy program. We find that disks around low-mass young stars show a negative radial gradient in the mass-averaged grain size and mass fraction of crystalline silicates. A positive correlation exists between the mass-averaged grain sizes of amorphous silicates and the accretion rates if the latter is above ~10^{-9} Msun/yr, possibly indicating that those disks are sufficiently turbulent to prevent grains of several microns in size to sink into the disk interior.Comment: 17 pages, 18 figures, 6 tables, language revised; accepted to A&

Safety hazards associated with the charging of lithium/sulfur dioxide cells

A continuing research program to assess the responses of spirally wound, lithium/sulfur dioxide cells to charging as functions of charging current, temperature, and cell condition prior to charging is described. Partially discharged cells that are charged at currents greater than one ampere explode with the time to explosion inversely proportional to the charging current. Cells charged at currents of less than one ampere may fail in one of several modes. The data allows an empirical prediction of when certain cells will fail given a constant charging current