Legumes as food ingredient: characterization, processing, and applications

Editores: Jiménez-López, José Carlos (CSIC); Clemente, Alfonso (CSIC

Discovering novel c-di-GMP activated EPS in bacteria

Resumen del poster presentado en: XIII Reunión del Grupo de Microbiología Molecular de La SEM. Granada, 7-9 septiembre (2022

Input Devices for DAS

This chapter gives an overview of the requirements for the input devices for driver assistance functions and the resulting design options: The reader is provided with a systematic procedure for designing input devices according to (Kircher JH, Baum E (Hrsg), Mensch-Maschine-Umwelt. Ergonomie für Konstrukteure, Designer, Planer und Arbeitsgestalter (Man-machine environment. Ergonomics for engineers, designers, planners and human factors engineers). Beuth Verlag GmbH, Berlin/Köln, 1986). This procedure will begin with identifying the requirements for driver assistance system (DAS) input devices, then follows an explanation of how which body part (ex. finger, hand, etc.), posture and grip type, for system interactions are determined. Additionally, it supports the selection of input devices and provides guidance of how to avoid accidental and unauthorized input. Finally it helps with the design and geometric integration of the arrangement, the definition of feedback and use direction, travel and resistance and the identification of controls. General recommendations are illustrated through specific examples of hardware, demonstrating how manifold input controls can be. In the last part of the chapter an overview of novel operation concepts is given, most of which are not currently implemented in vehicles, however, are estimated to gain importance in the future

Cluster binding studies with two anti-Thomsen-Friedenreich (anti-core-1, CD176, TF) antibodies: evidence for a multiple TF epitope

Antibodies to carbohydrate epitopes are often of the IgM isotype and require multiple binding for sufficient avidity. Therefore clusters of epitopes are preferred antigenic sites in these cases. We have examined the type of clusters recognized by two anti-Thomsen-Friedenreich (TF, core-1, CD176) IgM antibodies, NM-TF1 and NM-TF2, using several different sets of TF-carrying synthetic glycoconjugates in ELISA experiments. To our surprise, the single most important factor determining binding strength was a close vicinity of several TF glycans at distances of ≤1 nm. Considering the known dimensions of IgM antibodies, our data strongly suggest that a cluster of up to four TF moieties, presenting as a "multiple epitope", is required to attach to a single combining site in order to result in adequate binding strength. This effect can also be achieved by "surrogate-multiple epitopes" consisting of separate TF-carrying molecules in close vicinity. In addition, it was found that serine-linked TFs are stronger bound than threonine-linked TFs by both antibodies. This peculiar type of cluster recognition may contribute to improved avidity and explicit tumor specificity