Academic Service-Learning Experience Embraces the Vincentian Mission in Physician Assistant Education

Motivated by the belief that health care is a basic human right and driven by a Vincentian mission, St. John’s University Physician Assistant Program incorporates service minded educational experiences created to expose students to the necessity to reach those most in need—“the poor, alienated and the aged.” In Spring 2018, in conjunction with the SJU Academic Service-Learning program, a geriatric service assignment was integrated into the geriatric course curriculum. Working with Selfhelp Virtual Senior Center, local senior centers and nursing homes, students participated in an outreach project to serve senior citizens. Students shared their experiences through reflection and pre- and post-experience surveys. The reflections included open-ended questions to encourage students to examine and explore ideas regarding older patient’s unique needs to promote empathy and understanding. Conscious and subconscious prejudices and assumptions regarding “old people” were challenged. Students found that participants were more knowledgeable and willing to try new things than they thought. They also discovered that seniors were able to utilize technology and were much more informed than the students anticipated. Together with the teachings of St. Vincent, this AS-L program not only provided an opportunity for senior citizen participants to socialize with the community, but helped our students grow personally and professionally. The results and student reflections collected favor providing service opportunities to enhance the learning objectives of the geriatric course, as well as instill or reinforce compassion and service to aged underserved communities

Spatially-explicit individual based modeling of marine populations: A review of the advances in the 1990s

The utility of individual based models (IBMs) is that properties of ecological systems can be derived by considering the properties of individuals constituting them. Individual differences may be physiological, behavioral or may arise from interactions among individuals. The differences result in unique life histories, which when considered as a whole give rise to growth and size distributions that provide a measure of the state of the population. Early IBMs generally did not consider the effect of a spatially variable physical environment. Recent advances in ocean circulation models that include realistic temporal and spatial variation of currents, turbulence, light, prey, etc., have enabled IBMs to be embedded in model flow fields and for unique, sometimes behaviorally modified, Lagrangian trajectories to be computed. The explicit consideration of realistic spatial heterogeneity provides an additional factor that contributes to the differentiation among individuals, to variances in population structure, and ultimately to our understanding of the recruitment process. This is particularly important in marine environments where fronts, boundary layers, pycnoclines, gyres and other smaller spatial features have been hypothesized to play a significant role in determining vital rates and population structure. In this paper we will review the status of research on spatially-explicit IBMs, their successes, limitations and future developments. Examples will be drawn from approaches used in the past decade in GLOBEC, FOCI, SABRE and other programs

Psychotic versus nonpsychotic depression in hospitalized adolescents

One hundred fifty adolescent inpatients with major depression were systematically assessed for demographic and clinical differences between psychotic and nonpsychotic depression. Delusions and/or hallucinations were present in 10% of the subjects. The psychotic group had significantly more frequent and severe suicidal ideation. Posttraumatic stress disorder was also more frequent in the psychotic group. Depression and Anxiety 6:40–42, 1997. © 1997 Wiley-Liss, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/35214/1/6_ftp.pd

Larval Trophodynamics, Turbulence, and Drift on Georges Bank : A Sensitivity Analysis of Cod and Haddock

Using an individual-based model approach we consider trophodynamic effects on the growth and survival of larval cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) on Georges Bank during late winter/early spring. These studies represent an extension of results described in Werner et al. (1996; Deep-Sea Res. II), wherein the effect of turbulence-enhanced larval-prey contact rates increased the effective prey concentration resulting in growth of cod larvae consistent with observed rates in the field. We reformulated the feeding of the larvae to include existing relationships between maximum prey-length and larval-length and we examined: (i) larval search behaviour and its effect on encounter with prey, (ii) the ability of larvae to pursue and capture prey in a turbulent environment, and (iii) the effect of turbulence on the dispersion of larvae in the vertical. We find that search behaviour, the effect of turbulence on pursuit and capture, and vertical dispersion decrease the predicted larval growth rates compared to those observed in the earlier study. These results suggest that larval feeding behaviour, and especially the ability of larvae to pursue encountered prey, could be an important input to larval growth and survival models. The inclusion of turbulence in determining the position of passive larvae in the water column allows the larvae to sample the entire water column, contributing to a decrease in the variance of the size of the larvae over time. The ability of larvae to swim and aggregate in the vertical will be necessary to reproduce distributions observed in the field

Metabolomic profiling of amines in sepsis predicts changes in NOS canonical pathways

Rationale Nitric oxide synthase (NOS) is a biomarker/target in sepsis. NOS activity is driven by amino acids, which cycle to regulate the substrate L-arginine in parallel with cycles which regulate the endogenous inhibitors ADMA and L-NMMA. The relationship between amines and the consequence of plasma changes on iNOS activity in early sepsis is not known. Objective Our objective was to apply a metabolomics approach to determine the influence of sepsis on a full array of amines and what consequence these changes may have on predicted iNOS activity. Methods and measurements 34 amino acids were measured using ultra purification mass spectrometry in the plasma of septic patients (n = 38) taken at the time of diagnosis and 24–72 hours post diagnosis and of healthy volunteers (n = 21). L-arginine and methylarginines were measured using liquid-chromatography mass spectrometry and ELISA. A top down approach was also taken to examine the most changed metabolic pathways by Ingenuity Pathway Analysis. The iNOS supporting capacity of plasma was determined using a mouse macrophage cell-based bioassay. Main results Of all the amines measured 22, including L-arginine and ADMA, displayed significant differences in samples from patients with sepsis. The functional consequence of increased ADMA and decreased L-arginine in context of all cumulative metabolic changes in plasma resulted in reduced iNOS supporting activity associated with sepsis. Conclusions In early sepsis profound changes in amine levels were defined by dominant changes in the iNOS canonical pathway resulting in functionally meaningful changes in the ability of plasma to regulate iNOS activity ex vivo

Predicting cell types and genetic variations contributing to disease by combining GWAS and epigenetic data

Genome-wide association studies (GWASs) identify single nucleotide polymorphisms (SNPs) that are enriched in individuals suffering from a given disease. Most disease-associated SNPs fall into non-coding regions, so that it is not straightforward to infer phenotype or function; moreover, many SNPs are in tight genetic linkage, so that a SNP identified as associated with a particular disease may not itself be causal, but rather signify the presence of a linked SNP that is functionally relevant to disease pathogenesis. Here, we present an analysis method that takes advantage of the recent rapid accumulation of epigenomics data to address these problems for some SNPs. Using asthma as a prototypic example; we show that non-coding disease-associated SNPs are enriched in genomic regions that function as regulators of transcription, such as enhancers and promoters. Identifying enhancers based on the presence of the histone modification marks such as H3K4me1 in different cell types, we show that the location of enhancers is highly cell-type specific. We use these findings to predict which SNPs are likely to be directly contributing to disease based on their presence in regulatory regions, and in which cell types their effect is expected to be detectable. Moreover, we can also predict which cell types contribute to a disease based on overlap of the disease-associated SNPs with the locations of enhancers present in a given cell type. Finally, we suggest that it will be possible to re-analyze GWAS studies with much higher power by limiting the SNPs considered to those in coding or regulatory regions of cell types relevant to a given disease

Expression and membrane-targeting of an active plant cytochrome P450 in the chloroplast of the green alga Chlamydomonas reinhardtii

The unicellular green alga Chlamydomonas reinhardtii has potential as a cell factory for the production of recombinant proteins and other compounds, but mainstream adoption has been hindered by a scarcity of genetic tools and a need to identify products that can be generated in a cost-effective manner. A promising strategy is to use algal chloroplasts as a site for synthesis of high value bioactive compounds such as diterpenoids since these are derived from metabolic building blocks that occur naturally within the organelle. However, synthesis of these complex plant metabolites requires the introduction of membrane-associated enzymes including cytochrome P450 enzymes (P450s). Here, we show that a gene (CYP79A1) encoding a model P450 can be introduced into the C. reinhardtii chloroplast genome using a simple transformation system. The gene is stably expressed and the P450 is efficiently targeted into chloroplast membranes by means of its endogenous N-terminal anchor domain, where it is active and accounts for 0.4% of total cell protein. These results provide proof of concept for the introduction of diterpenoid synthesis pathways into the chloroplast of C. reinhardtii

Reconstitution of neutral amino acid transport from partially purified membrane components from ehrlich ascites tumor cells

Solubilized protein fractions have been obtained from plasma membranes of Ehrlich ascites cells either by extraction with 0.5% Triton X-100 or by extraction with 2% cholate. Partial purification of the solubilized protein fraction has been obtained by utilizing a combination of ammonium sulfate precipitation and column chromatography. Leucine-binding activity has been detected in the Triton X-100 solubilized membrane fraction. The leucine-binding activity was measured by equilibrium dialysis and was saturable with high levels of leucine or phenylalanine and is not strongly effected by alanine. These properties are similar to those previously identified as System L. In addition, the cholate extracted protein fraction was partially purified and reconstituted into liposomes. Sodium dependent uptake of alanine and leucine could be demonstrated in the reconstituted vesicles. Concentrative uptake was dependent upon a sodium gradient. A membrane potential produced by valinomycin mediated potassium diffusion in the presence of sodium also stimulated amino acid transport in reconstituted liposomes.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/38206/1/400070317_ftp.pd