“Pilot implementation of an interdisciplinary course on climate solutions”

A pilot implementation of an experimental interdisciplinary course on climate solutions was undertaken at San Jose´ State University in the fall semester of 2008. The course, co-taught by seven faculty members from six colleges, was approved for a general education requirement and was open to upperclass students campus-wide. A course with such a breadth of topics and range of student backgrounds was the first of its kind here. The lessons learned from the pilot effort were assessed from student, faculty, and administrative perspectives. The educational benefits to students from the interdisciplinary format were found to be substantial, in addition to faculty development. However, challenges associated with team-teaching were also encountered and must be overcome for the long-term viability of the course. The experimental course was approved as a permanent course starting in the fall semester of 2009 based on the pilot effort, and plays a role in the College of Engineering’s recent initiatives in sustainability in addition to campus-wide general educatio

Four stories on wildlife and sound

Thesis (S.M. in Science Writing)--Massachusetts Institute of Technology, Dept. of Humanities, Graduate Program in Science Writing, 2007.Includes bibliographical references (leaves 43-44).Typically, if scientists want to study animals in the wild they rely on field observations by eye. If they want to track those species to know where they are, where they are going, and how they behave, then researchers may capture and tag them. These methods, however, are difficult if not impossible for rare and hard-to-see species like whales in the ocean, elephants under a forest canopy, or birds at night. Sound gives scientists a new way of knowing what is swimming, roaming, and flying where. And some scientists are using these sounds for conservation, to identify the habitats animals need to survive and to protect the animals from human activity. Of course, as with any new science, there are unanswered questions. The uncertainties are especially profound in the ocean, where researchers know little about how marine creatures hear. Scientists are still searching for answers, but now they have a new way to find them.by Elizabeth H. Quill.S.M.in Science Writin

Satellite DNA as a target for TaqMan real-time PCR detection of the pinewood nematode, Bursaphelenchus xylophilus

The pinewood nematode (PWN), Bursaphelenchus xylophilus , is a major pathogen of conifers, which impacts on forest health, natural ecosystem stability and international trade. As a consequence, it has been listed as a quarantine organism in Europe. A real-time PCR approach based on TaqMan chemistry was developed to detect this organism. Specific probe and primers were designed based on the sequence of the Msp I satellite DNA family previously characterized in the genome of the nematode. The method proved to be specific in tests with target DNA from PWN isolates from worldwide origin. From a practical point of view, detection limit was 1 pg of target DNA or one individual nematode. In addition, PWN genomic DNA or single individuals were positively detected in mixed samples in which B. xylophilius was associated with the closely related non-pathogenic species B. mucronatus , up to the limit of 0.01% or 1% of the mixture, respectively. The real-time PCR assay was also used in conjunction with a simple DNA extraction method to detect PWN directly in artificially infested wood samples. These results demonstrate the potential of this assay to provide rapid, accurate and sensitive molecular identification of the PWN in relation to pest risk assessment in the field and quarantine regulation

Investigating the association of rs2910164 with cancer predisposition in an Irish cohort.

IntroductionMicroRNAs (miRNAs) are small noncoding RNA molecules that exert post-transcriptional effects on gene expression by binding with cis-regulatory regions in target messenger RNA (mRNA). Polymorphisms in genes encoding miRNAs or in miRNA-mRNA binding sites confer deleterious epigenetic effects on cancer risk. miR-146a has a role in inflammation and may have a role as a tumour suppressor. The polymorphism rs2910164 in the MIR146A gene encoding pre-miR-146a has been implicated in several inflammatory pathologies, including cancers of the breast and thyroid, although evidence for the associations has been conflicting in different populations. We aimed to further investigate the association of this variant with these two cancers in an Irish cohort.MethodsThe study group comprised patients with breast cancer (BC), patients with differentiated thyroid cancer (DTC) and unaffected controls. Germline DNA was extracted from blood or from saliva collected using the DNA Genotek Oragene 575 collection kit, using crystallisation precipitation, and genotyped using TaqMan-based PCR. Data were analysed using SPSS, v22.ResultsThe total study group included 1516 participants. This comprised 1386 Irish participants; 724 unaffected individuals (controls), 523 patients with breast cancer (BC), 136 patients with differentiated thyroid cancer (DTC) and three patients with dual primary breast and thyroid cancer. An additional cohort of 130 patients with DTC from the South of France was also genotyped for the variant. The variant was detected with a minor allele frequency (MAF) of 0.19 in controls, 0.22 in BC and 0.27 and 0.26 in DTC cases from Ireland and France, respectively. The variant was not significantly associated with BC (per allele odds ratio = 1.20 (0.98-1.46), P  = 0.07), but was associated with DTC in Irish patients (per allele OR = 1.59 (1.18-2.14), P = 0.002).ConclusionThe rs2910164 variant in MIR146A is significantly associated with DTC, but is not significantly associated with BC in this cohort

Electrochemical formation of nanoporosity in n-InP anodes in KOH

We review our recent work on anodic formation of nanoporosity in n-InP in aqueous KOH. Typically, a nanoporous sub-surface region is formed beneath a thin, dense near-surface layer. Atomic force microscopy (AFM) shows pit formation on the surface in the earlier stages of etching, and transmission electron microscopy (TEM) shows individual nanoporous domains separated from the surface by a thin InP layer. Each domain develops from a surface pit. We developed a model based on propagation of pores along the A directions. The model predicts porous domains with a truncated tetrahedral shape and this was confirmed by scanning electron microscopy (SEM) and TEM. Pores are cylindrical and have well-developed facets only near their tips. No porous layers are observed at a KOH concentration of 1.1 mol dm -2 or lower. Linear sweep voltammograms (LSVs) show a pronounced anodic peak corresponding to the formation of the porous region. We describe a technique to deconvolute the effects of potential and time in LSVs and explain their shape and their relationship to porous layer formation

Development of improved structural adhesives Annual summary report, 1 Jul. 1967 - 3 Dec. 1968

Improved structural adhesives for bonding aluminum over low temperature

Mechanism that dictates pore width and <111>a pore propagation in InP

We report a mechanism for pore growth and propagation based on a three-step charge transfer model. The study is supported by electron microscopy analysis of highly doped n-InP samples anodised in aqueous KOH. The model and experimental data are used to explain propagation of pores of characteristic diameter preferentially along the A directions. We also show evidence for deviation of pore growth from the A directions and explain why such deviations should occur. The model is self-consistent and predicts how carrier concentration affects the internal dimensions of the porous structures

Deconvolution of the potential and time dependence of electrochemical porous semiconductor formation

A layer of porous InP is grown beneath a thin dense surface layer when n-InP electrodes are anodized to sufficiently high potentials in aqueous KOH solutions. The shape of the linear sweep (LSV) or the cyclic voltammogram (CV) is dependent on carrier concentration. A technique is presented to deconvolute the effects of potential and time on a CV. The results obtained from this technique are used to explain the shape of the anodic current response and its relation to porous layer formation. The accuracy of the deconvolution technique is then tested by comparison to experimental results

Preferential <111>A pore propagation mechanism in n-InP anodized in KOH

This paper describes the formation of pores during the anodization of n-InP in aqueous KOH. The pores propagate preferentially along the A crystallographic directions and form truncated tetrahedral domains. A model is presented that explains preferential A pore propagation and the uniform diameters of pores. The model outlines how pores can deviate from the A directions and from their characteristic diameters. It also details the effect of variation of carrier concentration on the dimensions of the porous structures

Thermally Resistant Polymers for Use as Fuel Tanks Sealants Annual Summary Report, 1 Jul. 1968 - 30 Jun. 1969

Siloxane and perfluoroalkylenic polymers for elastomeric fuel tank sealant material