Accurate spectroscopy of Sr atoms

We report the frequency measurement with an accuracy in the 100 kHz range of several optical transitions of atomic Sr : $^1S_0- ^3P_1$ at 689 nm, $^3P_1- ^3S_1$ at 688 nm and $^3P_0- ^3S_1$ at 679 nm. Measurements are performed with a frequency chain based on a femtosecond laser referenced to primary frequency standards. They allowed the indirect determination with a 70 kHz uncertainty of the frequency of the doubly forbidden 5s^2^1S_0- 5s5p^3P_0 transition of $^{87}$Sr at 698 nm and in a second step its direct observation. Frequency measurements are performed for $^{88}$Sr and $^{87}$Sr, allowing the determination of $^3P_0$, $^3P_1$ and $^3S_1$ isotope shifts, as well as the $^3S_1$ hyperfine constants.Comment: 12 pages, 16 figure

The unstructured linker arms of MutL enable GATC site incision beyond roadblocks during initiation of DNA mismatch repair

DNA mismatch repair (MMR) maintains genome stability through repair of DNA replication errors. In Escherichia coli, initiation of MMR involves recognition of the mismatch by MutS, recruitment of MutL, activation of endonuclease MutH and DNA strand incision at a hemimethylated GATC site. Here, we studied the mechanism of communication that couples mismatch recognition to daughter strand incision. We investigated the effect of catalytically-deficient Cas9 as well as stalled RNA polymerase as roadblocks placed on DNA in between the mismatch and GATC site in ensemble and single molecule nanomanipulation incision assays. The MMR proteins were observed to incise GATC sites beyond a roadblock, albeit with reduced efficiency. This residual incision is completely abolished upon shortening the disordered linker regions of MutL. These results indicate that roadblock bypass can be fully attributed to the long, disordered linker regions in MutL and establish that communication during MMR initiation occurs along the DNA backbone