Pembentukan 8-OHdG Dari Zat Toksik Pemicu Radikal Bebas

This study was conducted to observethe profile of DNA Adduct (8-OHdG) formation as DNA damage indicators, by using calf thymus DNA incubated with toxic and carcinogenic compounds. The compounds which could trigger free radicals in this research were PAH(Benzo[a]Pyrene), TiO2, and CuCl2. Calf thymus DNA was incubated with Benzo[a]Pyrene and CuCl2 compounds under pH and temperature variations. The incubation of calf thymus DNA with TiO2-UV radiation (254 nm) wasused to induce the formation of reactive oxygen species (ROS) in the process of oxidative DNA damage. From this research, all of compounds have potency to trigger the formation of DNA Adduct (8-OHdG). The ratio of absorbance to assess the purity of DNA at 260 nm and 280 nm (λ260/ λ280 ) was measured at ~1.9. The shifted peaks at λmax were indicating changes on structures of DNA as a result of calf thymus DNA incubation with B[a]P and CuCl2. The highest level of 8-OHdG results in calf thymus DNA incubation with B[a]P and CuCl2 under pH 8.5 and incubation temperature at 60°C, was about 120.856 μg/L. Calf thymus DNA incubation with TiO2-UV radiation (254 nm) under pH 8.5 resulting 8-OHdG level at 57.025 μg/L

Coexistence of superconductivity with exotic ferromagnetic state in pressurized non-superconducting UTe2_2

The discovery of superconductivity in heavy Fermion UTe$_2$, a candidate topological and triplet-paired superconductor, has aroused widespread interest. However, to date, superconductivity has only been reported in nonstoichiometric crystals of UTe$_2$ with a Te deficit. Here, we demonstrate that the application of uniaxial pressure induces superconductivity in stoichiometric UTe$_2$ crystals. Measurements of resistivity, magnetoresistance and susceptibility reveal that uniaxial pressure results in a suppression of the Kondo coherent state seen at ambient pressure, leading to the emergence of superconductivity initially at 1.5 GP, followed by the development of bulk superconductivity at 4.8 GPa. The superconducting state coexists with an exotic ferromagnetically ordered (FM) state that develops just below the onset temperature of the superconducting transition. High-pressure synchrotron x-ray diffraction measurements performed at 20 K indicate that no structural phase transition occurs over the measured pressure range. Our results not only demonstrate the coexistence of superconductivity with an exotic ferromagnetic state in pressurized stoichiometric UTe$_2$, but also highlight a vital role of Te deficiency in developing superconductivity at ambient pressures.Comment: 17 pages, 4 figure

Automatic Recognition of Laryngoscopic Images Using a Deep-Learning Technique

Objectives/Hypothesis: To develop a deep-learning–based computer-aided diagnosis system for distinguishing laryngeal neoplasms (benign, precancerous lesions, and cancer) and improve the clinician-based accuracy of diagnostic assessments of laryngoscopy findings. Study Design: Retrospective study. Methods: A total of 24,667 laryngoscopy images (normal, vocal nodule, polyps, leukoplakia and malignancy) were collected to develop and test a convolutional neural network (CNN)-based classifier. A comparison between the proposed CNN-based classifier and the clinical visual assessments (CVAs) by 12 otolaryngologists was conducted. Results: In the independent testing dataset, an overall accuracy of 96.24% was achieved; for leukoplakia, benign, malignancy, normal, and vocal nodule, the sensitivity and specificity were 92.8% vs. 98.9%, 97% vs. 99.7%, 89% vs. 99.3%, 99.0% vs. 99.4%, and 97.2% vs. 99.1%, respectively. Furthermore, when compared with CVAs on the randomly selected test dataset, the CNN-based classifier outperformed physicians for most laryngeal conditions, with striking improvements in the ability to distinguish nodules (98% vs. 45%, P <.001), polyps (91% vs. 86%, P <.001), leukoplakia (91% vs. 65%, P <.001), and malignancy (90% vs. 54%, P <.001). Conclusions: The CNN-based classifier can provide a valuable reference for the diagnosis of laryngeal neoplasms during laryngoscopy, especially for distinguishing benign, precancerous, and cancer lesions. Level of Evidence: NA Laryngoscope, 130:E686–E693, 2020

Rediscovery of the lost little dogwood Cornus wardiana (Cornaceae)—Its phylogenetic and morphological distinction and implication in the origin of the Arctic-Sino-Himalayan disjunction

The dwarf dogwoods (subgenus Arctocrania) have been widely known to consist of three circumboreal species Cornus suecica, Cornus canadensis, and Cornus unalaschkensis. A fourth putative species was discovered from the northern Myanmar in 1937, but it had never been formally reported on. Here, we formally report the species on the basis of phylogenetic and morphological evidence and name it Cornus wardiana Rushforth & Wahlsteen (sp. nov.). We conducted phylogenetic and morphometric analyses to determine its evolutionary relationship and differentiation from the existing relatives. We dated the phylogeny using molecular data and conducted a biogeographic analysis to gain insights into the evolution and biogeography of the Arctic-Sino-Himalayan disjunction. The phylogenetic analysis used sequences of the nrITS and plastid matK and rbcL genes and included all four dwarf dogwoods and 20 other species representing the three other major lineages of Cornus and the outgroup. The morphometric analyses included 60 populations and 102 specimens of dwarf dogwood, representing the entire range of the subgenus. The results showed that C. wardiana diverged first within subgenus Arctocrania in the Miocene, from a wide-spread ancestor. Results from principal component analysis and discriminant analysis also showed that the Myanmar samples are well separated from the others. Taken together, these results suggest that the dwarf dogwood lineage split from the big-bracted dogwoods in Asia or Asia-western North America during the late Paleocene and spread widely to form a Eurasia-North America distribution; the Arctic-Sino-Himalayan disjunction was the result of southward migration in the Miocene followed by extinction in the intervening highland areas

Mechanisms Underlying the Antidepressant Response of Acupuncture via PKA/CREB Signaling Pathway

Protein kinase A (PKA)/cAMP response element-binding (CREB) protein signaling pathway, contributing to impaired neurogenesis parallel to depressive-like behaviors, has been identified as the crucial factor involved in the antidepressant response of acupuncture. However, the molecular mechanisms associated with antidepressant response of acupuncture, neurogenesis, and depressive-like behaviors ameliorating remain unexplored. The objective was to identify the mechanisms underlying the antidepressant response of acupuncture through PKA signaling pathway in depression rats by employing the PKA signaling pathway inhibitor H89 in in vivo experiments. Our results indicated that the expression of hippocampal PKA-α and p-CREB was significantly downregulated by chronic unpredicted mild stress (CUMS) procedures. Importantly, acupuncture reversed the downregulation of PKA-α and p-CREB. The expression of PKA-α was upregulated by fluoxetine, but not p-CREB. No significant difference was found between Acu and FLX groups on the expression of PKA-α and p-CREB. Interestingly, H89 inhibited the effects of acupuncture or fluoxetine on upregulating the expression of p-CREB, but not PKA-α. There was no significant difference in expression of CREB among the groups. Conclusively, our findings further support the hypothesis that acupuncture could ameliorate depressive-like behaviors by regulating PKA/CREB signaling pathway, which might be mainly mediated by regulating the phosphorylation level of CREB

Light-Up Nonthiolated Aptasensor for Low-Mass, Soluble Amyloid‑β₄₀ Oligomers at High Salt Concentrations

Herein, a light-up nonthiolated aptasensor was developed for low-mass, soluble amyloid-β₄₀ oligomers (LS-Aβ_40‑O). Au nanoparticles (AuNP) were employed as colorimetric probes, and the nonthiolated aptamers (Apt) were adsorbed on AuNP surfaces, acting as binding elements for LS-Aβ_40‑O. The aggregation of AuNPs was induced when Apt-modified AuNPs (Apt@AuNPs) were under high-salt conditions. However, upon the addition of LS-Aβ_40‑O into the Apt@AuNP solution, the salt tolerance of the AuNPs was greatly enhanced. Further studies confirmed that the formed LS-Aβ_40‑O–Apt complex attached onto the AuNP surfaces via interactions between LS-Aβ_40‑O and Au, which led to electrostatic and steric stabilization of the AuNPs under high-salt conditions. On the basis of this outcome, a sensitive light-up nonthiolated aptasensor for LS-Aβ_40‑O was achieved with a detection limit of 10.0 nM and a linear range from 35.0 to 700 nM in a 175 mM NaCl solution. Cerebrospinal-fluid (CSF) samples from healthy persons and Alzheimer’s disease (AD) patients were successfully distinguished by using this proposed method. The concentrations of LS-Aβ_40‑O in the CSF of AD patients were of nanomolar grade, but there was no detectable LS-Aβ_40‑O in those of the healthy persons. This work provides a new insight into the interaction between Apt@AuNPs and Aβ_40‑O and also develops a simple, rapid, highly selective and sensitive, and applicable method for LS-Aβ_40‑O detection in real CSF samples, which is significant for the diagnosis of AD

Frequency distributions of the ages and <i>Ka/Ks</i> values through time within a 200 million year window using combined data from all eight <i>Cornus</i> species and the frequency distributions of the dates of WGD in the <i>Cornus</i> ancestor inferred from gene tree method.

<p>a. The frequency distributions of the ages of paralogous genes. The normal distribution components of ages estimated using EMMIX and colored according to those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171361#pone.0171361.g003" target="_blank">Fig 3</a>. The vertical dashed lines represent average absolute ages of corresponding components (or WGD). b. <i>Ka/Ks</i> values through time within a 200 million year window. The grey bars represent the average values of <i>Ka/Ks</i> in a increment of 5 myr. and evident increase of <i>Ka/Ks</i> values after the inferred WGD event (marked by the blue vertical line and black bars). c. The frequency distributions of the dates of gene duplications at the <i>Cornus</i> ancestral node (inferred as the WGD event) from 98 gene trees. The normal distribution components of dates estimated using EMMIX. The vertical blue dashed line represent average dates of component.</p

Summary of assembly for transcriptomes of ten species.

<p>Summary of assembly for transcriptomes of ten species.</p