Identification of a Surface Protein from Lactobacillus reuteri JCM1081 That Adheres to Porcine Gastric Mucin and Human Enterocyte-Like HT-29 Cells

Adhesion of lactobacilli to the host gastrointestinal (GI) tract is considered an important factor in health-promoting effects. However, studies addressing the molecular mechanisms of the adhesion of lactobacilli to the host GI tract have not yet been performed. The aim of this work was to identify Lactobacillus reuteri surface molecules mediating adhesion to intestinal epithelial cells and mucins. Nine strains of lactobacilli were tested for their ability to adhere to human enterocyte-like HT-29 cells. The cell surface proteins involved in the adhesion of Lactobacillus to HT-29 cells and gastric mucin were extracted. The active fractions were detected by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western blotting with horseradish peroxidase-labeled mucin and NHS-Biotin-labeled HT-29 cells. Furthermore, tandem mass spectrometry analysis was performed to identify the surface protein that participates in adhesion. It was shown that the ability of lactobacilli to adhere to HT-29 cells in vitro varied considerably among different strains. The most adhesive strain was the chicken intestinal tract isolate Lactobacillus reuteri JCM1081 (495.07 ± 80.03 bacterial cells/100 HT-29 cells). The adhesion of L. reuteri JCM1081 to HT-29 cells appeared to be mediated by a cell surface protein, with an approximate molecular mass of 29 kDa. The peptides generated from the 29-kDa protein significantly matched the Lr0793 protein sequence of L. reuteri strain ATCC55730 (∼71.1% identity) and displayed significant sequence similarity to the putative ATP-binding cassette transporter protein CnBP

Chemical composition of the essential oil of whole plant of Elsholtizia dense Benth and its anti-tumor effect on human hepatoma cells

Purpose: To determine the chemical components of the essential oil of Elsholtizia dense in Sichuan Province and evaluate the effect of the oil on human hepatoma cells (SMMC-7721) in vitro.Methods: The essential oil was extracted using the modified steam-distillation  extraction method, and its chemical components were determined by gas  chromatography-mass spectrometry (GC-MS). The effect of the essential oil on proliferation of SMMC-7721 cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, with L02 and HeLa cells serving as control groups.Results: GC-MS results show that the essential oil of E. dense contains 40  components. Thirty seven components were identified and accounted for 98.39 % of the essential oil. The two main components were rosefuran epoxide (53.12 %) and 2-ethyl imidazole (29.8 %). The oil significantly inhibited cell proliferation in a concentration- and time-dependent manner (p < 0.05). SMMC-7721 cells were more inhibited than L02 and HeLa cells by the oil, with half maximal inhibitory concentration (IC50) values of 26.23 and 25.46 μg/mL after 8-h and 24-h treatments, respectively.Conclusion: Out of the 40 chemical components of the essential oil of E. dense, rosefuran epoxide and 2-ethyl imidazole were the most abundant. The oil has a significant anti-tumor effect on SMMC-7721 cells, and thus has a potential to be developed as an anti-liver cancer drug.Keywords: Medicinal herb, Elsholtizia dense Benth, Essential oil, Rosefuran epoxide, 2-Ethyl imidazole, Anti-tumor activit

1,2;5,6-Di-O-isopropyl­idene-3-C-nitro­methyl-α-d-allofuran­ose

The mol­ecule of the title compound, C13H21NO8, consists of two methyl­enedi­oxy rings and one tetra­hydro­furan ring. In the crystal, inter­molecular O—H⋯O hydrogen bonds link the mol­ecules into helical chains running along the 61 screw axis. Weak inter­molecular C—H⋯O hydrogen bonds help to stabilize the crystal packing. Voids of 245 Å3 per unit cell occur

Removal of As(V) and As(III) from aqueous solution using hydrous ceric oxide

Removal of As(V) and As(III) from water using hydrous ceric oxide, CeO(2)center dot xH(2)O (HCO) was investigated under different pH and As loading conditions, using batch equilibrium adsorption and FTIR methods. Adsorption of both As(V) and As(III) anions was virtually independent of pH and up to 100% removal can be achieved in the lower concentration range 0.5 - 5.0 mg L(-1) As at sorbent dosage of 1.0 g L(-1). As the initial As concentration increased to 50, 100 or 250 mg L(-1) for the same sorbent dosage, distinct adsorption maxima of As(V) appeared and shift to lower pH, whereas that of As(III) was found at approximately pH 8. The effect of contact time was dependent on pH but adsorption equilibriums were reached after 6 h in all cases for the studied systems. The isotherms fit well in the Langmuir model of adsorption. Both As(V) and As(III) anions were adsorbed on HCO principally by forming inner-sphere complexes as revealed by the FTIR spectra

3,6-Didehydro-5-hy­droxy-1,2-O-iso­propyl­idene-5-C-nitro­meth­yl-α-d-gluco­furan­ose

The title compound, C10H15NO7, consists of one methyl­enedi­oxy ring and two fused tetra­hydro­furan rings. The three fused rings exhibit cis arrangements at the ring junctions. One O atom of a tetra­hydro­furan ring and the H atoms bound to the neighboring C atoms are disordered over two orientations with site-occupancy factors of 0.69 (1) and 0.31 (1). intra­molecular O—H⋯O and C—H⋯O inter­actions stabilize the mol­ecular conformation. In the crystal structure, inter­molecular O—H⋯O and C—H⋯O inter­actions link the mol­ecules into a three-dimensional network

IPC02-27155 DEVELOPMENT OF LARGE DIAMETER X70 HIGH TOUGHNESS HSAW LINEPIPE FOR GAS TRANSMMISION

ABSTRACT X70 large diameter linepipe with helical seam SAW were developed, with1016mm OD and 14.6mm WT. Acicular ferrite type linepipe steel is adopted for the base material, which was found having high toughness and low yield strength loss after pipe forming. The very stringent requirements for toughness, i.e. 190J/140J for average/minimum for pipe body and 120J/90J for average/minimum for weld and HAZ were meet successfully. The yield strength loss due to Bauschinger effect was found lower than 20 MPa, which benefited

In Vivo Disruption of TGF-β Signaling by Smad7 in Airway Epithelium Alleviates Allergic Asthma but Aggravates Lung Carcinogenesis in Mouse

BACKGROUND: TGF-beta has been postulated to play an important role in the maintenance of epithelial homeostasis and the development of epithelium-derived cancers. However, most of previous studies are mainly focused on the function of TGF-beta in immune cells to the development of allergic asthma and how TGF-beta signaling in airway epithelium itself in allergic inflammation is largely unknown. Furthermore, the in vivo TGF-beta function specifically in the airway epithelium during lung cancer development has been largely elusive. METHODOLOGY/PRINCIPAL FINDINGS: To evaluate the in vivo contribution of TGF-beta signaling in lung epithelium to the development of allergic disease and lung cancer, we generated a transgenic mouse model with Smad7, an intracellular inhibitor of TGF-beta signaling, constitutively expressed in mouse airway Clara cells using a mouse CC10 promoter. The mice were subjected to the development of OVA-induced allergic asthma and urethane-induced lung cancer. The Smad7 transgenic animals significantly protected from OVA-induced asthma, with reduced airway inflammation, airway mucus production, extracellular matrix deposition, and production of OVA-specific IgE. Further analysis of cytokine profiles in lung homogenates revealed that the Th2 cytokines including IL-4, IL-5 and IL-13, as well as other cytokines including IL-17, IL-1, IL-6, IP10, G-CSF, and GM-CSF were significantly reduced in the transgenic mice upon OVA induction. In contrast, the Smad7 transgenic animals had an increased incidence of lung carcinogenesis when subjected to urethane treatment. CONCLUSION/SIGNIFICANCE: These studies, therefore, demonstrate for the first time the in vivo function of TGF-beta signaling specifically in airway epithelium during the development of allergic asthma and lung cancer

Dumbbell shaped craniorbital cavernous hemangioma.

BACKGROUND: Cavernous hemangioma of the orbit is a benign tumor mostly located behind the eye globe, but it rarely spread into the brain, which is called cerebral cavernous malformation as well, the lesion in the brain is irregular and enlarged blood. Here we report one particular case of craniorbital cavernous hemangioma. CASE PRESENTATION: A 53-year-old woman presented with exophthalmos of the right eye and reduced vision. Computerized tomographical (CT) scan showed osteolytic honeycomb radial changes of the outer plate of the skull. A magnetic resonance imaging (MRI) scan was performed to obtain further details. T1-weighted (T1W) imaging showed slightly low signal mixed with small patchy high signal. T2-weighted (T2W) imaging showed uneven high signal. There was obvious enhancement in the middle and no enhancement in the peripheral bars. A surgically manage was performed using a left frontotemporal approach, the tumor excised fully, and the histopathology results revealed a cavernous hemangioma. The patient recovered well in the follow-up. Post-operative CT scan identified the lesion was successfully resected, MRI scan also showed full resection and enhanced signal from the presence of fat. CONCLUSIONS: Craniorbital cavernous hemangioma is uncommon, however within the cranium, they can lead to numerous complications particularly if affecting the visual apparatus. it could be diagnosed by imaging, which CT scan shows osteolytic honeycomb radial changes of the outer plate of the skull, T1W imaging shows slightly low signal mixed with small patchy high signal, T2W imaging shows uneven high signal, it is obvious enhancement in the middle and no enhancement in the peripheral bars. The surgically manage is the ideally treatment when there are some symptoms