Pathophysiology and transcriptomic analysis of Picea koraiensis inoculated by bark beetle-vectored fungus Ophiostoma bicolor

Ophiostomatoid fungi exhibit a complex relationship with bark beetles; exhausting of host tree defenses is traditionally regarded as one of the key benefits provided to beetle vectors. Ophiostoma bicolor is one of the dominant species of the mycobiota associated with Ips genus bark beetles which infect the spruce trees across the Eurasian continent. Host spruce trees resist fungal invasion through structural and inducible defenses, but the underlying mechanisms at the molecular level, particularly with respect to the interaction between bark beetle-associated fungi and host trees, remain unclear. The aim of this study was to observe the pathological physiology and molecular changes in Picea koraiensis seedlings after artificial inoculation with O. bicolor strains (TS, BH, QH, MX, and LWQ). This study showed that O. bicolor was a weakly virulent pathogen of spruce, and that the virulent of the five O. bicolor strains showed differentiation. All O. bicolor strains could induce monoterpenoid release. A positive correlation between fungal virulence and release of monoterpenoids was observed. Furthermore, the release rate of monoterpenoids peaked at 4 days post-inoculation (dpi) and then decreased from 4 to 90 dpi. Transcriptomic analysis at 4 dpi showed that many plant-pathogen interaction processes and mitogen-activated protein kinase (MAPK) metabolic processes were activated. The expression of monoterpenoid precursor synthesis genes and diterpenoid synthesis genes was upregulated, indicating that gene expression regulated the release rate of monoterpenoids at 4 dpi. The enriched pathways may reveal the immune response mechanism of spruce to ophiostomatoid fungi. The dominant O. bicolor possibly induces the host defense rather than defense depletion, which is likely the pattern conducted by the pioneers of beetle-associated mycobiota, such as Endoconidiophora spp.. Overall, these results facilitate a better understanding of the interaction mechanism between the dominant association of beetles and the host at the molecular level

Design and Evaluate Coordinated Ramp Metering Strategies for Utah Freeways

MPC-641During the past few decades, ramp metering control has been widely implemented in many U.S. states, including Utah. Numerous studies and applications have demonstrated that ramp metering control is an effective strategy to reduce overall freeway congestion by managing the amount of traffic entering the freeway. Ramp metering controllers can be implemented as coordinated or uncoordinated systems. Currently, Utah freeway on-ramps are operated in an uncoordinated way. Despite improvements to the operational efficiency of mainline flows, uncoordinated ramp metering will inevitably create additional delays to the ramp flows. Therefore, this project aims to assist the Utah Department of Transportation (UDOT) in deploying coordinated ramp metering systems and evaluating the performance of deployed systems. First, we leverage a method to identify existing freeway bottlenecks using current UDOT datasets, including PeMs and ClearGuide. Based on this, we select the site that may benefit from coordinated ramp metering from those determined locations. A VISSIM model is then developed for this selected corridor and the VISSIM model is calibrated based on collected traffic flow data. We apply the calibrated VISSIM model to conduct simulations to evaluate system performance under different freeway mainline congestion levels. Finally, the calibrated VISSIM model is leveraged to evaluate the coordinated ramp metering strategy of the bottleneck algorithm from both operational and safety aspects

Three-dimensional reconstruction optimization of tunnel face and intelligent extraction of discontinuity orientation based on binocular stereo vision

In the process of grading and dynamically optimizing the design and construction parameters of the surrounding rock mass of a rock tunnel face, efficiently and accurately acquiring the geometrical parameters of the rock discontinuities is an important basic task. To address the problems of time consuming, low accuracy, and high danger associated with traditional methods of obtaining the structural information of rock mass, this paper proposes a method for three-dimensional reconstruction and intelligent information extraction of tunnel face based on binocular stereo vision (BSV). First, the parallel binocular device with a single camera was improved, calibrated using the checkerboard calibration method. By integrating with the semi-global matching algorithm, the BSV based method for the three-dimensional reconstruction of the rock mass of the tunnel face was optimized. Furthermore, based on the results from on-site engineering applications, this study leveraged two parameters, point cloud density and algorithm runtime, to determine the optimal values for the disparity range and window size parameters within the semi-global stereo matching algorithm. This enhancement improved the performance of the 3D reconstruction method based on binocular stereo vision. Finally, efficient and refined intelligent methods for extracting structural parameters of the rock mass were proposed based on k-nearest neighbor search and kernel density estimation. The research results can provide reliable technical support for the intelligent and efficient acquisition of rock mass structural information in rock tunnel engineering faces

Removal of Hepatitis B virus surface HBsAg and core HBcAg antigens using microbial fuel cells producing electricity from human urine

© 2019, The Author(s). Microbial electrochemical technology is emerging as an alternative way of treating waste and converting this directly to electricity. Intensive research on these systems is ongoing but it currently lacks the evaluation of possible environmental transmission of enteric viruses originating from the waste stream. In this study, for the first time we investigated this aspect by assessing the removal efficiency of hepatitis B core and surface antigens in cascades of continuous flow microbial fuel cells. The log-reduction (LR) of surface antigen (HBsAg) reached a maximum value of 1.86 ± 0.20 (98.6% reduction), which was similar to the open circuit control and degraded regardless of the recorded current. Core antigen (HBcAg) was much more resistant to treatment and the maximal LR was equal to 0.229 ± 0.028 (41.0% reduction). The highest LR rate observed for HBsAg was 4.66 ± 0.19 h−1 and for HBcAg 0.10 ± 0.01 h−1. Regression analysis revealed correlation between hydraulic retention time, power and redox potential on inactivation efficiency, also indicating electroactive behaviour of biofilm in open circuit control through the snorkel-effect. The results indicate that microbial electrochemical technologies may be successfully applied to reduce the risk of environmental transmission of hepatitis B virus but also open up the possibility of testing other viruses for wider implementation

A Decrease in the Staminode-Mediated Visitor Screening Mechanism in Response to Nectar Robbers Positively Affects Reproduction in Delphinium caeruleum Jacq. ex Camb. (Ranunculaceae)

Nectar-robbing insects, which are frequently described as cheaters in plant–pollinator mutualisms, may affect plant reproductive fitness by obtaining nectar rewards without providing pollination services. The negative effects of nectar robbing on plant reproductive success have been widely reported, but the reasons for possible positive effects demand further investigation. The goal of the study was to evaluate the effects of nectar robbing on the reproductive success of Delphinium caeruleum. Two staminodes cover the stamens and pistils in the flowers of D. caeruleum, forming a “double door” type of structure that compels pollinators to physically manipulate the staminodes to access the sex organs. In order to explore whether the operative strength required to open the staminodes is affected by actions associated with nectar robbing, we set up five different treatment groups: no nectar robbing, natural nectar robbing, artificial nectar robbing, hole making, and nectar removal. A biological tension sensor was used to measure the operative strength required to open the staminodes in the flowers. We also assessed the effect of nectar robbing on the flower-visiting behavior of pollinators and the effect of nectar robbing on reproductive fitness by the flower. The results showed that the operative strength needed to open staminodes was reduced by nectar robbers but not by artificial nectar robbing, hole making, or nectar removal. The flowers’ continuous visitation rate and visitation frequency by pollinators decreased significantly in robbed flowers. Both the pollen export and pollen deposition in naturally robbed flowers were significantly higher than those in nonrobbed flowers. Our results demonstrate that nectar robbers play an indirect positive role in the reproductive fitness of D. caeruleum flowers by reducing the operative strength of staminodes to promote pollen transfer. The reduction in operative strength of staminodes might be an adaptive mechanism that responds to nectar robbing

Connected Vehicle System Design for Signalized Arterials

It can be expected that connected vehicles (CVs) systems will soon go beyond testbed and appear in real-world applications. To accommodate a large number of connected vehicles on the roads, traffic signal control systems on signalized arterials would require supports of various components such as roadside infrastructure, vehicle on-board devices, an effective communication network, and optimal control algorithms. In this project, we aim to establish a real-time and adaptive system for supporting the operations of CV-based traffic signal control functions. The proposed system will prioritize the communication needs of different types of CVs and best utilize the capacity of the communication channels. The CV data sensing and acquisition protocol, built on a newly developed concept of Age of Information (AoI), will support the feedback control loop to adjust signal timing plans. Our multidisciplinary research team, including researchers from transportation engineering and electrical engineering, will carry out the project tasks along four directions that capitalized on the PIs’ expertise: (i) Data collection and communication, in which the proposed system will be based on the AoI, prioritize the data needs of different types of CVs, and optimize the communication network; (ii) Dynamic traffic signal coordination, which will concurrently facilitate the progression of traffic flows along multiple critical paths; (iii) Smart traffic signal control, where both operational efficiency and safety improvement are accounted for at signalized intersections; and (iv) Multimodal system design, which will integrate transit signal priority (TSP) and suppression controls for accommodating connected buses. This project addresses the urgent needs in CV system designs and offers control foundations to support the operations of urban signalized arterial in a CV environment