Molecular Lines of 13 Galactic Infrared Bubble Regions

We investigated the physical properties of molecular clouds and star formation processes around infrared bubbles which are essentially expanding HII regions. We performed observations of 13 galactic infrared bubble fields containing 18 bubbles. Five molecular lines, 12CO (J=1-0), 13CO (J=1-0), C18O(J=1-0), HCN (J=1-0), and HCO+ (J=1-0), were observed, and several publicly available surveys, GLIMPSE, MIPSGAL, ATLASGAL, BGPS, VGPS, MAGPIS, and NVSS, were used for comparison. We find that these bubbles are generally connected with molecular clouds, most of which are giant. Several bubble regions display velocity gradients and broad shifted profiles, which could be due to the expansion of bubbles. The masses of molecular clouds within bubbles range from 100 to 19,000 solar mass, and their dynamic ages are about 0.3-3.7 Myr, which takes into account the internal turbulence pressure of surrounding molecular clouds. Clumps are found in the vicinity of all 18 bubbles, and molecular clouds near four of these bubbles with larger angular sizes show shell-like morphologies, indicating that either collect-and-collapse or radiation-driven implosion processes may have occurred. Due to the contamination of adjacent molecular clouds, only six bubble regions are appropriate to search for outflows, and we find that four of them have outflow activities. Three bubbles display ultra-compact HII regions at their borders, and one of them is probably responsible for its outflow. In total, only six bubbles show star formation activities in the vicinity, and we suggest that star formation processes might have been triggered.Comment: 55 Pages, 32 figures. Accepted for publication in A

Thrusting and exhumation of the southern Mongolian Plateau: Joint thermochronological constraints from the Langshan Mountains, western Inner Mongolia, China

The Mongolian Plateau has undergone multi-stage denudation since the Late Triassic, and the NE-trending Langshan Mountains in the southern margin of the Mongolian Plateau is crucial to unraveling the Meso-Cenozoic cooling and exhumation history of the Mongolian Plateau. The Langshan Mountains are dominated by Precambrian gneiss and Permian–Middle Triassic granitic plutons crosscut by a set of NE-striking thrust faults. A joint thermochronological study was conducted on 31 granitic and gneissic samples along the HQ and CU transects across the Langshan Mountains and other two samples from the BQ in the north of the Langshan Mountains. Four biotite/muscovite and three K-feldspar 40Ar/39Ar plateau ages range from 205 ± 1 to 161 ± 1 and 167 ± 1 to 131 ± 1 Ma, respectively. Thirty-three apatite fission track (AFT) ages are between 184 ± 11 and 79 ± 4 Ma, with mean track lengths from 11.1 ± 1.8 to 13.1 ± 1.4 μm of mostly unimodal distributions. Thirty-one single-grain raw AHe ages are in a range of 134 ± 8 to 21 ± 1 Ma. The AFT ages decrease monotonously from NW to SE until thrust faults along the two transects, with an age-jump across thrust F35. Joint thermal history modelling shows a three-stage cooling history as a result of denudation, especially with spatial differentiation in the first stage. Relative slow cooling at c. 0.6–1.0 °C/Ma occurred in the BQ and the northern part of the HQ transect during 220–100 Ma and the northern part of the CU transect during 160–100 Ma, respectively, with an amount of c. 2–3 km denudation between 160 and 100 Ma, implying little movement along the thrusts F13 and F33. In the middle and southern parts of the HQ transect and the southern part of the CU transect, rapid cooling at c. 4.0–7.0 °C/Ma, with c. 6–9 km denudation during 170–130 or 160–100 Ma, respectively, is probably influenced by thrusting of F35, F38 and F42 and the resultant tilting. A combination of thrusting, tilting, and denudation led to the youngering trends towards thrusts in different parts. However, there was no significant denudation across the Langshan Mountains in the second stage from c. 100 or 80 Ma until the last stage of rapid denudation (c. 2 km) since 20–10 Ma, which is simultaneous with the rapid uplift of the northern part of the Tibetan Plateau at c. 15 Ma. A youngering trend of AFT ages from the inner to the peripherals of the Mongolian Plateau implies the outward propagation of the Mongolian Plateau since the Mesozoic

The Distances to Molecular Clouds at High Galactic Latitudes based on GAIA DR2

We report the distances of molecular clouds at high Galactic latitudes (|b|>10$^\circ$) derived from parallax and G band extinction (A$_{\rm G}$) measurements in the second Gaia data release, Gaia DR2. Aided by Bayesian analyses, we determined distances by identifying the breakpoint in the extinction A$_{\rm G}$ towards molecular clouds and using the extinction A$_{\rm G}$ of Gaia stars around molecular clouds to confirm the breakpoint. We use nearby star-forming regions, such as Orion, Taurus, Cepheus, and Perseus, whose distances are well-known to examine the reliability of our method. By comparing with previous results, we found that the molecular cloud distances derived from this method are reliable. The systematic error in the distances is approximately 5%. In total, 52 molecular clouds have their distances well determined, most of which are at high Galactic latitudes, and we provide reliable distances for 13 molecular clouds for the first time.Comment: Accepted by A&A. 13 pages, 9 figure

Molecular Tweezers-like Calix[4]arene Based Alkaline Earth Metal Cation (Ca2+, Sr2+, and Ba2+) Chemosensor and Its Imaging in Living Cells and Zebrafish

Although alkaline earth metal cations play an important role in our daily life, little attention has been paid to the field of fast quantitative analysis of their content due to a lack of satisfactory precision and a fast and convenient means of detection. In this study, we have designed a set of molecular tweezers based on the calix[4]arene chemosensor L, which was found to exhibit high selectivity and sensitivity toward Ca2+, Sr2+, and Ba2+ (by UV-vis and fluorescence methods) with low detection limits of the order of 10-7 to 10-8 M and high association constants (of the order of 106). More significantly, sensor L not only can recognize Ca2+, Sr2+, and Ba2+ but also can further discriminate between these three cations via the differing red shifts in their UV-vis spectra (560 nm for L·Ca2+, 570 nm for L·Sr2+, and 580 nm for L·Ba2+ complex) which is attributed to their different atomic radii. A rare synergistic effect for the recognition mechanism has been demonstrated by 1H NMR spectroscopic titration. Sensor L constructed a high shielding field by the cooperation of Tris with alkaline earth metal ion after complex. Additionally, the presence of acetoxymethyl group in sensor L results in enhancement of cell permeability, and as a consequence, sensor L exhibited excellent sensing and imaging (in vivo) in living cells and in zebrafish

DNA-PKcs plays a dominant role in the regulation of H2AX phosphorylation in response to DNA damage and cell cycle progression

<p>Abstract</p> <p>Background</p> <p>When DNA double-strand breaks (DSB) are induced by ionizing radiation (IR) in cells, histone H2AX is quickly phosphorylated into γ-H2AX (p-S139) around the DSB site. The necessity of DNA-PKcs in regulating the phosphorylation of H2AX in response to DNA damage and cell cycle progression was investigated.</p> <p>Results</p> <p>The level of γH2AX in HeLa cells increased rapidly with a peak level at 0.25 - 1.0 h after 4 Gy γ irradiation. SiRNA-mediated depression of DNA-PKcs resulted in a strikingly decreased level of γH2AX. An increased γH2AX was also induced in the ATM deficient cell line AT5BIVA at 0.5 - 1.0 h after 4 Gy γ rays, and this IR-increased γH2AX in ATM deficient cells was dramatically abolished by the PIKK inhibitor wortmannin and the DNA-PKcs specific inhibitor NU7026. A high level of constitutive expression of γH2AX was observed in another ATM deficient cell line ATS4. The alteration of γH2AX level associated with cell cycle progression was also observed. HeLa cells with siRNA-depressed DNA-PKcs (HeLa-H1) or normal level DNA-PKcs (HeLa-NC) were synchronized at the G1 phase with the thymidine double-blocking method. At ~5 h after the synchronized cells were released from the G1 block, the S phase cells were dominant (80%) for both HeLa-H1 and HeLa-NC cells. At 8 - 9 h after the synchronized cells released from the G1 block, the proportion of G2/M population reached 56 - 60% for HeLa-NC cells, which was higher than that for HeLa H1 cells (33 - 40%). Consistently, the proportion of S phase for HeLa-NC cells decreased to ~15%; while a higher level (26 - 33%) was still maintained for the DNA-PKcs depleted HeLa-H1 cells during this period. In HeLa-NC cells, the γH2AX level increased gradually as the cells were released from the G1 block and entered the G2/M phase. However, this γH2AX alteration associated with cell cycle progressing was remarkably suppressed in the DNA-PKcs depleted HeLa-H1 cells, while wortmannin and NU7026 could also suppress this cell cycle related phosphorylation of H2AX. Furthermore, inhibition of GSK3β activity with LiCl or specific siRNA could up-regulate the γH2AX level and prolong the time of increased γH2AX to 10 h or more after 4 Gy. GSK3β is a negative regulation target of DNA-PKcs/Akt signaling via phosphorylation on Ser9, which leads to its inactivation. Depression of DNA-PKcs in HeLa cells leads to a decreased phosphorylation of Akt on Ser473 and its target GSK3β on Ser9, which, in other words, results in an increased activation of GSK3β. In addition, inhibition of PDK (another up-stream regulator of Akt/GSK3β) by siRNA can also decrease the induction of γH2AX in response to both DNA damage and cell cycle progression.</p> <p>Conclusion</p> <p>DNA-PKcs plays a dominant role in regulating the phosphorylation of H2AX in response to both DNA damage and cell cycle progression. It can directly phosphorylate H2AX independent of ATM and indirectly modulate the phosphorylation level of γH2AX via the Akt/GSK3 β signal pathway.</p

Association between gut microbiota and gastrointestinal cancer: a two-sample bi-directional Mendelian randomization study

BackgroundThe gut microbiome is closely related to gastrointestinal (GI) cancer, but the causality of gut microbiome with GI cancer has yet to be fully established. We conducted this two-sample Mendelian randomization (MR) study to reveal the potential causal effect of gut microbiota on GI cancer.Materials and methodsSummary-level genetic data of gut microbiome were derived from the MiBioGen consortium and the Dutch Microbiome Project. Summary statistics of six GI cancers were drawn from United Kingdom Biobank. Inverse-variance-weighted (IVW), MR-robust adjusted profile score (MR-RAPS), and weighted-median (WM) methods were used to evaluate the potential causal link between gut microbiota and GI cancer. In addition, we performed sensitivity analyses and reverse MR analyses.ResultsWe identified potential causal associations between 21 bacterial taxa and GI cancers (values of p &lt; 0.05 in all three MR methods). Among them, phylum Verrucomicrobia (OR: 0.17, 95% CI: 0.05–0.59, p = 0.005) retained a strong negative association with intrahepatic cholangiocarcinoma after the Bonferroni correction, whereas order Bacillales (OR: 1.67, 95% CI: 1.23–2.26, p = 0.001) retained a strong positive association with pancreatic cancer. Reverse MR analyses indicated that GI cancer was associated with 17 microbial taxa in all three MR methods, among them, a strong inverse association between colorectal cancer and family Clostridiaceae1 (OR: 0.91, 95% CI: 0.86–0.96, p = 0.001) was identified by Bonferroni correction.ConclusionOur study implicates the potential causal effects of specific microbial taxa on GI cancer, potentially providing new insights into the prevention and treatment of GI cancer through specific gut bacteria