The Location Privacy Preserving of Social Network Based on RCCAM Access Control

Location-based services in social networks provide much convenience for people but bring much risk of location privacy disclosure. Aiming at this problem, a location privacy preservation algorithm based on RCCAM access control model is proposed to assign the accessing users of the access permission and the visibility level of location information through the combination of conflict resolution strategy, permission allocation strategy and location generalization strategy. RCCAM is a relationship-based multi-users cooperation access control model, which takes the same shared contents that may involves the privacy profits of multi-users into consideration. The core of the algorithm is the value of open tendency which depends on the location sensitivity and the intimacy between the users. The conflict resolution strategy adopts the value of open tendency to vote for concessions. The permission allocation strategy and location generalization strategy to obtain the specific access permission and the location visibility level for accessing users according to the value of open tendency. The algorithm can achieve fine-grained control of location publishing of the shared content which involves stakeholder's privacy profit and maintain the sharing will of promulgator as possible

Effects of ABA and CaCl₂ on GABA accumulation in fava bean germinating under hypoxia-NaCl stress

<div><p>Effects of exogenous abscisic acid (ABA) and CaCl₂ on γ-aminobutyric acid (GABA) accumulation of germinated fava bean under hypoxia-NaCl stress were investigated. Exogenous ABA resulted in the enhancement of glutamate decarboxylase (GAD) and diamine oxidase (DAO) activity as well as GABA content in cotyledon and shoot. CaCl₂ increased both enzyme activities in shoot and GABA content in cotyledon and shoot. ABA downregulated <i>GAD</i> expression in cotyledon and radicle, while upregulated that in shoot; it also upregulated <i>DAO</i> expression in each organ. CaCl₂ upregulated <i>GAD</i> expression in cotyledon, while downregulated that in radicle. However, it upregulated <i>DAO</i> expression in shoot, downregulated that in radicle. ABA inhibitor fluridon and ethylenediaminetetraacetic acid inhibited GAD and DAO activities significantly so that inhibited GABA accumulation through reducing ABA biosynthesis and chelating Ca²⁺, respectively. However, they upregulated <i>GAD</i> and <i>DAO</i> expression in varying degrees. These results indicate that ABA and Ca²⁺ participate in GABA biosynthesis in fava bean during germination under hypoxia-NaCl stress.</p></div

β-Elemene Reverses Gefitinib Resistance in NSCLC Cells by Inhibiting lncRNA H19-Mediated Autophagy

Lung cancer is a leading cause of mortality worldwide, especially among Asian patients with non-small cell lung cancer (NSCLC) who have epidermal growth factor receptor (EGFR) mutations. Initially, first-generation EGFR tyrosine kinase inhibitors (TKIs) are commonly administered as the primary treatment option; however, encountering resistance to these medications poses a significant obstacle. Hence, it has become crucial to address initial resistance and ensure continued effectiveness. Recent research has focused on the role of long noncoding RNAs (lncRNAs) in tumor drug resistance, especially lncRNA H19. β-elemene, derived from Curcuma aromatic Salisb., has shown strong anti-tumor effects. However, the relationship between β-elemene, lncRNA H19, and gefitinib resistance in NSCLC is unclear. This study aims to investigate whether β-elemene can enhance the sensitivity of gefitinib-resistant NSCLC cells to gefitinib and to elucidate its mechanism of action. The impact of gefitinib and β-elemene on cell viability was evaluated using the cell counting kit-8 (CCK8) assay. Furthermore, western blotting and qRT-PCR analysis were employed to determine the expression levels of autophagy-related proteins and genes, respectively. The influence on cellular proliferation was gauged through a colony-formation assay, and apoptosis induction was quantified via flow cytometry. Additionally, the tumorigenic potential in vivo was assessed using a xenograft model in nude mice. The expression levels of LC3B, EGFR, and Rab7 proteins were examined through immunofluorescence. Our findings elucidate that the resistance to gefitinib is intricately linked with the dysregulation of autophagy and the overexpression of lncRNA H19. The synergistic administration of β-elemene and gefitinib markedly attenuated the proliferative capacity of resistant cells, expedited apoptotic processes, and inhibited the in vivo proliferation of lung cancer. Notably, β-elemene profoundly diminished the expression of lncRNA H19 and curtailed autophagic activity in resistant cells, thereby bolstering their responsiveness to gefitinib. Moreover, β-elemene disrupted the Rab7-facilitated degradation pathway of EGFR, facilitating its repositioning to the plasma membrane. β-elemene emerges as a promising auxiliary therapeutic for circumventing gefitinib resistance in NSCLC, potentially through the regulation of lncRNA H19-mediated autophagy. The participation of Rab7 in this dynamic unveils novel insights into the resistance mechanisms operative in lung cancer, paving the way for future therapeutic innovations

Negative and Positive Bias for Emotional Faces: Evidence from the Attention and Working Memory Paradigms

Visual attention and visual working memory (VWM) are two major cognitive functions in humans, and they have much in common. A growing body of research has investigated the effect of emotional information on visual attention and VWM. Interestingly, contradictory findings have supported both a negative bias and a positive bias toward emotional faces (e.g., angry faces or happy faces) in the attention and VWM fields. We found that the classical paradigms—that is, the visual search paradigm in attention and the change detection paradigm in VWM—are considerably similar. The settings of these paradigms could therefore be responsible for the contradictory results. In this paper, we compare previous controversial results from behavioral and neuroscience studies using these two paradigms. We suggest three possible contributing factors that have significant impacts on the contradictory conclusions regarding different emotional bias effects; these factors are stimulus choice, experimental setting, and cognitive process. We also propose new research directions and guidelines for future studies

Negative and Positive Bias for Emotional Faces : Evidence from the Attention and Working Memory Paradigms

Visual attention and visual working memory (VWM) are two major cognitive functions in humans, and they have much in common. A growing body of research has investigated the effect of emotional information on visual attention and VWM. Interestingly, contradictory findings have supported both a negative bias and a positive bias toward emotional faces (e.g., angry faces or happy faces) in the attention and VWM fields. We found that the classical paradigms—that is, the visual search paradigm in attention and the change detection paradigm in VWM—are considerably similar. The settings of these paradigms could therefore be responsible for the contradictory results. In this paper, we compare previous controversial results from behavioral and neuroscience studies using these two paradigms. We suggest three possible contributing factors that have significant impacts on the contradictory conclusions regarding different emotional bias effects; these factors are stimulus choice, experimental setting, and cognitive process. We also propose new research directions and guidelines for future studies.peerReviewe

Mechanism of Calcium Lactate Facilitating Phytic Acid Degradation in Soybean during Germination

Calcium lactate facilitates the growth and phytic acid degradation of soybean sprouts, but the mechanism is unclear. In this study, calcium lactate (Ca) and calcium lactate with lanthanum chloride (Ca+La) were used to treat soybean sprouts to reveal the relevant mechanism. Results showed that the phytic acid content decreased and the availability of phosphorus increased under Ca treatment. This must be due to the enhancement of enzyme activity related to phytic acid degradation. In addition, the energy metabolism was accelerated by Ca treatment. The energy status and energy metabolism-associated enzyme activity also increased. However, the transmembrane transport of calcium was inhibited by La³⁺ and concentrated in intercellular space or between the cell wall and cell membrane; thus, Ca+La treatment showed reverse results compared with those of Ca treatment. Interestingly, gene expression did not vary in accordance with their enzyme activity. These results demonstrated that calcium lactate increased the rate of phytic acid degradation by enhancing growth, phosphorus metabolism, and energy metabolism

Fuzzy piecewise coordinated control and stability analysis of the photovoltaic‐storage direct current microgrid

Abstract In order to ensure the safe and reliable operation of photovoltaic‐storage DC microgrid in islanded mode, this paper proposes a piecewise coordinated control strategy based on fuzzy logic to independently judge the working mode of microgrid. Firstly, according to the operation characteristics of microgrid, the device‐level control of each unit (photovoltaic, energy storage and load) is designed successively. Then, according to the requirement of supply and demand balance of microgrid, fuzzy logic is introduced to realize the independent division of threshold nodes on the basis of piecewise control, so as to ensure the fast switching of working mode and stable operation of the system in various complex environments, that is system‐level control. And the method of impedance analysis is used to analyze the stability of microgrid under different working modes. Finally, the effectiveness and feasibility of the proposed coordination control strategy are verified by MATLAB/Simulink simulation results

Hyperactivation and enhanced cytotoxicity of reduced CD8+ gamma delta T cells in the intestine of patients with Crohn’s disease correlates with disease activity

Abstract Background and aims We aimed to investigate the immune characteristics of intestinal CD8+ gamma delta T (CD8+ γδ T) cells in Crohn’s disease (CD) and their correlation with disease activity. Methods The study cohorts included 21 CD patients and 21 healthy individuals. CD8+ γδ T cells were isolated from human ileal mucosa for detection by flow cytometry. The activation or inhibition status of cells was detected by detecting the expression of activation marker HLA-DR and the immunosuppressive molecule PD-1 on cells. The cytotoxicity of cells was assessed by detecting the expression of cytotoxic molecules (Perforin, Granzyme B, and TRAIL) in cells. Ratios of investigated cells were calculated as prediction factors by receiver operating characteristic curve (ROC) analysis. Results The study revealed a reduction in intestinal CD8+ γδT cells among active CD patients, with a more pronounced reduction observed in moderately active patients compared to mildly active patients. Moreover, active CD patients exhibited heightened activation levels in their intestinal CD8+ γδT cells, whereas the activation was comparatively weakened in moderately active patients compared with mildly active patients. Additionally, the cytotoxicity of intestinal CD8+ γδT cells was enhanced solely in mildly active patients, while it was impaired in moderately active patients compared with mildly active patients. Furthermore, HLA-DR+ CD8+ γδT cell ratio, CD8+ γδT ratio, and CD8+ γδT count were identified as indicators in the diagnosis of active CD. Meanwhile, the ratios of Granzyme B+ CD8+ γδT cell and Perforin+ CD8+ γδT cell were identified as indicators that distinguish mildly moderately active CD cases. Conclusions Intestinal CD8+ γδT was reduced in active CD patients, but their activation and cytotoxicity were enhanced. However, with increased disease activity, intestinal CD8+ γδ T cells became dysfunctional. CD-specific perturbations observed in various phenotypic markers in CD8+ γδ T cells can be used as indicators to assist in diagnosing CD patients