1,352 research outputs found
Expressions of ECE-CYC2 clade genes relating to abortion of both dorsal and ventral stamens in Opithandra (Gesneriaceae)
<p>Abstract</p> <p>Background</p> <p>ECE-CYC2 clade genes known in patterning floral dorsoventral asymmetry (zygomorphy) in <it>Antirrhinum majus </it>are conserved in the dorsal identity function including arresting the dorsal stamen. However, it remains uncertain whether the same mechanism underlies abortion of the ventral stamens, an important morphological trait related to evolution and diversification of zygomorphy in Lamiales <it>sensu lato</it>, a major clade of predominantly zygomorphically flowered angiosperms. <it>Opithandra </it>(Gesneriaceae) is of particular interests in addressing this question as it is in the base of Lamiales <it>s.l</it>., an early representative of this type zygomorphy.</p> <p>Results</p> <p>We investigated the expression patterns of four ECE-CYC2 clade genes and two putative target <it>cyclinD3 </it>genes in <it>Opithandra </it>using RNA <it>in situ </it>hybridization and RT-PCR. <it>OpdCYC </it>gene expressions were correlated with abortion of both dorsal and ventral stamens in <it>Opithandra</it>, strengthened by the negatively correlated expression of their putative target <it>OpdcyclinD3 </it>genes. The complement of <it>OpdcyclinD3 </it>to <it>OpdCYC </it>expressions further indicated that <it>OpdCYC </it>expressions were related to the dorsal and ventral stamen abortion through negative effects on <it>OpdcyclinD3 </it>genes.</p> <p>Conclusion</p> <p>These results suggest that ECE-CYC2 clade TCP genes are not only functionally conserved in the dorsal stamen repression, but also involved in arresting ventral stamens, a genetic mechanism underlying the establishment of zygomorphy with abortion of both the dorsal and ventral stamens evolved in angiosperms, especially within Lamiales <it>s.l</it>.</p
Downstream of tyrosine kinase/docking protein 6, as a novel substrate of tropomyosin-related kinase C receptor, is involved in neurotrophin 3-mediated neurite outgrowth in mouse cortex neurons
<p>Abstract</p> <p>Background</p> <p>The downstream of tyrosine kinase/docking protein (Dok) adaptor protein family has seven members, Dok1 to Dok7, that act as substrates of multiple receptor tyrosine kinase and non-receptor tyrosine kinase. The tropomyosin-related kinase (Trk) receptor family, which has three members (TrkA, TrkB and TrkC), are receptor tyrosine kinases that play pivotal roles in many stages of nervous system development, such as differentiation, migration, axon and dendrite projection and neuron patterning. Upon related neurotrophin growth factor stimulation, dimerisation and autophosphorylation of Trk receptors can occur, recruiting adaptor proteins to mediate signal transduction.</p> <p>Results</p> <p>In this report, by using yeast two-hybrid assays, glutathione S-transferase (GST) precipitation assays and coimmunoprecipitation (Co-IP) experiments, we demonstrate that Dok6 selectively binds to the NPQY motif of TrkC through its phosphotyrosine-binding (PTB) domain in a kinase activity-dependent manner. We further confirmed their interaction by coimmunoprecipitation and colocalisation in E18.5 mouse cortex neurons, which provided more <it>in vivo </it>evidence. Next, we demonstrated that Dok6 is involved in neurite outgrowth in mouse cortex neurons via the RNAi method. Knockdown of Dok6 decreased neurite outgrowth in cortical neurons upon neurotrophin 3 (NT-3) stimulation.</p> <p>Conclusions</p> <p>We conclude that Dok6 interacts with the NPQY motif of the TrkC receptor through its PTB domain in a kinase activity-dependent manner, and works as a novel substrate of the TrkC receptor involved in NT-3-mediated neurite outgrowth in mouse cortex neurons.</p
Insulin regulates glucose consumption and lactate production through reactive oxygen species and pyruvate kinase M2.
Although insulin is known to regulate glucose metabolism and closely associate with liver cancer, the molecular mechanisms still remain to be elucidated. In this study, we attempt to understand the mechanism of insulin in promotion of liver cancer metabolism. We found that insulin increased pyruvate kinase M2 (PKM2) expression through reactive oxygen species (ROS) for regulating glucose consumption and lactate production, key process of glycolysis in hepatocellular carcinoma HepG2 and Bel7402 cells. Interestingly, insulin-induced ROS was found responsible for the suppression of miR-145 and miR-128, and forced expression of either miR-145 or miR-128 was sufficient to abolish insulin-induced PKM2 expression. Furthermore, the knockdown of PKM2 expression also inhibited cancer cell growth and insulin-induced glucose consumption and lactate production, suggesting that PKM2 is a functional downstream effecter of insulin. Taken together, this study would provide a new insight into the mechanism of insulin-induced glycolysis
Measurement-device-independent quantum key distribution over untrustful metropolitan network
Quantum cryptography holds the promise to establish an
information-theoretically secure global network. All field tests of
metropolitan-scale quantum networks to date are based on trusted relays. The
security critically relies on the accountability of the trusted relays, which
will break down if the relay is dishonest or compromised. Here, we construct a
measurement-device-independent quantum key distribution (MDIQKD) network in a
star topology over a 200 square kilometers metropolitan area, which is secure
against untrustful relays and against all detection attacks. In the field test,
our system continuously runs through one week with a secure key rate ten times
larger than previous result. Our results demonstrate that the MDIQKD network,
combining the best of both worlds --- security and practicality, constitutes an
appealing solution to secure metropolitan communications.Comment: 17 pages, 4 figure
Current reversals and metastable states in the infinite Bose-Hubbard chain with local particle loss
We present an algorithm which combines the quantum trajectory approach to
open quantum systems with a density-matrix renormalization group scheme for
infinite one-dimensional lattice systems. We apply this method to investigate
the long-time dynamics in the Bose-Hubbard model with local particle loss
starting from a Mott-insulating initial state with one boson per site. While
the short-time dynamics can be described even quantitatively by an equation of
motion (EOM) approach at the mean-field level, many-body interactions lead to
unexpected effects at intermediate and long times: local particle currents far
away from the dissipative site start to reverse direction ultimately leading to
a metastable state with a total particle current pointing away from the lossy
site. An alternative EOM approach based on an effective fermion model shows
that the reversal of currents can be understood qualitatively by the creation
of holon-doublon pairs at the edge of the region of reduced particle density.
The doublons are then able to escape while the holes move towards the
dissipative site, a process reminiscent---in a loose sense---of Hawking
radiation
N-Phenylpyridine-2-carbÂamide
In the title compound, C12H10N2O, the dihedral angle between the pyridine ring system and the phenyl ring is 1.8 (1)°. There is an intraÂmolecular N—H⋯N hydrogen bond between the pyridine N atom and the amide NH function
Real-space BCS-BEC crossover in FeSe monolayer
The quantum many body states in the BCS-BEC crossover regime are of
long-lasting interest. Here we report direct spectroscopic evidence of BCS-BEC
crossover in real-space in a FeSe monolayer thin film by using spatially
resolved scanning tunneling spectra. The crossover is driven by the shift of
band structure relative to the Fermi level. The theoretical calculation based
on a two-band model qualitatively reproduces the measured spectra in the whole
crossover range. In addition, the Zeeman splitting of the quasi-particle states
is found to be consistent with the characteristics of a condensate. Our work
paves the way to study the exotic states of BCS-BEC crossover in a
two-dimensional crystalline material at the atomic scale
Genomic Inference of the Metabolism and Evolution of the Archaeal Phylum Aigarchaeota
Microbes of the phylum Aigarchaeota are widely distributed in geothermal environments, but their physiological and ecological roles are poorly understood. Here we analyze six Aigarchaeota metagenomic bins from two circumneutral hot springs in Tengchong, China, to reveal that they are either strict or facultative anaerobes, and most are chemolithotrophs that can perform sulfide oxidation. Applying comparative genomics to the Thaumarchaeota and Aigarchaeota, we find that they both originated from thermal habitats, sharing 1154 genes with their common ancestor. Horizontal gene transfer played a crucial role in shaping genetic diversity of Aigarchaeota and led to functional partitioning and ecological divergence among sympatric microbes, as several key functional innovations were endowed by Bacteria, including dissimilatory sulfite reduction and possibly carbon monoxide oxidation. Our study expands our knowledge of the possible ecological roles of the Aigarchaeota and clarifies their evolutionary relationship to their sister lineage Thaumarchaeota
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