Properties and Applications of Geopolymer Composites: A Review Study of Mechanical and Microstructural Properties

Portland cement (PC) is considered the most energy-intensive building material and contributes to around 10% of global warming. It exacerbates global warming and climate change, which have a harmful environmental impact. Efforts are being made to produce sustainable and green concrete as an alternative to PC concrete. As a result, developing a more sustainable strategy and eco-friendly materials to replace ordinary concrete has become critical. Many studies on geopolymer concrete, which has equal or even superior durability and strength compared to traditional concrete, have been conducted for this purpose by many researchers. Geopolymer concrete (GPC) has been developed as a possible new construction material for replacing conventional concrete, offering a clean technological choice for long-term growth. Over the last few decades, geopolymer concrete has been investigated as a feasible green construction material that can reduce CO2 emissions because it uses industrial wastes as raw materials. GPC has proven effective for structural applications due to its workability and analogical strength compared to standard cement concrete. This review article discusses the engineering properties and microstructure of GPC and shows its merits in construction applications with some guidelines and suggestions recommended for both the academic community and the industrial sector. This literature review also demonstrates that the mechanical properties of GPC are comparable and even sometimes better than those of PC concrete. Moreover, the microstructure of GPC is significantly different from that of PC concrete microstructure and can be affected by many factors

Investigation of the physical-mechanical properties and durability of high-strength concrete with recycled PET as a partial replacement for fine aggregates

In this study, PET plastic waste, which is a type of polymer commonly used in the manufacture of plastic bottles, has been incorporated into concrete by partially replacing the natural fine aggregate. An experimental study was conducted by casting and testing 90 concrete cylinders and 54 concrete cubes. A concrete mixture was designed in which the natural fine aggregate was substituted partially with PET plastic waste (PW) at a ratio of 0%, 25%, and 50%, with various w/c ratios of.40,.45, and.55. Physical, mechanical, and durability properties were assessed. The downside of the test results show degradation in each of the following characteristics: slump, compressive strength, splitting tensile strength, ultrasonic pulse velocity, water absorption, and porosity. The degradation of these characteristics increased with the increase in the volume of plastic aggregate (PA) and the w/c ratio. While the positive side of the results showed that with the increase of the PA volume and the w/c ratio, the fresh and dry densities decreased further, and by using 50% PET, the dry density became below 2000 kg/m3. Therefore, it is classified as lightweight concrete. Moreover, the fracture of concrete changed from brittle to more ductile compared to control concrete. Also, the thermal conductivity decreased significantly (11%–47%), and by using 50% of PET, the thermal conductivity became less than.71 W/mK, and accordingly, classified as a bearing insulator

FITBAR: a web tool for the robust prediction of prokaryotic regulons

<p>Abstract</p> <p>Background</p> <p>The binding of regulatory proteins to their specific DNA targets determines the accurate expression of the neighboring genes. The <it>in silico </it>prediction of new binding sites in completely sequenced genomes is a key aspect in the deeper understanding of gene regulatory networks. Several algorithms have been described to discriminate against false-positives in the prediction of new binding targets; however none of them has been implemented so far to assist the detection of binding sites at the genomic scale.</p> <p>Results</p> <p>FITBAR (Fast Investigation Tool for Bacterial and Archaeal Regulons) is a web service designed to identify new protein binding sites on fully sequenced prokaryotic genomes. This tool consists in a workbench where the significance of the predictions can be compared using different statistical methods, a feature not found in existing resources. The Local Markov Model and the Compound Importance Sampling algorithms have been implemented to compute the P-value of newly discovered binding sites. In addition, FITBAR provides two optimized genomic scanning algorithms using either log-odds or entropy-weighted position-specific scoring matrices. Other significant features include the production of a detailed genomic context map for each detected binding site and the export of the search results in spreadsheet and portable document formats. FITBAR discovery of a high affinity <it>Escherichia coli </it>NagC binding site was validated experimentally <it>in vitro </it>as well as <it>in vivo </it>and published.</p> <p>Conclusions</p> <p>FITBAR was developed in order to allow fast, accurate and statistically robust predictions of prokaryotic regulons. This feature constitutes the main advantage of this web tool over other matrix search programs and does not impair its performance. The web service is available at <url>http://archaea.u-psud.fr/fitbar</url>.</p

Stabilization of Soft Soil by a Sustainable Binder Comprises Ground Granulated Blast Slag (GGBS) and Cement Kiln Dust (CKD)

Due to its significant deficiencies such as low permeability, low bearing and shear strength, and excessive compressibility, soft soil is one of the most problematic types of soil in civil engineering and soil stabilization can be considered a suitable technique for pavements. This study investigates the use of ground granulated blast slag (GGBS) and cement kiln dust (CKD) as stabilizers for soft soil. Thus, this study involves two optimization stages; in the first stage, GGBS was incorporated into 0%, 3%, 6%, 9%, and 12% by the weight of cement to obtain the optimal percentage, which was 6%. Then, the optimal GGBS was blended with CKD in a binary system at 0%, 25%, 50%, 75%, and 100% by the dry weight of the soil. The testing program used in this paper was Atterberg limits with compaction parameters to investigate the physical properties and unconfined compressive strength (USC) at 7 and 28 days to examine the mechanical characteristics. In addition, the microstructures of the soil specimens were tested at 7 and 28 days using scanning electron microscopy (SEM). The findings reveal that the binary system enhanced the physical and mechanical properties of the soft soil. The optimum binder achieved in this study was 6% (25% GGBS and 75% CKD), which generates an increase in strength of about 3.3 times in 7 days, and of 5.5 times in 28 days in comparison to the untreated soil. The enhancement was attributed to the formation of the hydration products as approved by SEM. Consequently, in the case of soft subgrade soils, this technique can increase the pavement’s bearing capacity and performance

Modulation of host cell processes by T3SS effectors

Two of the enteric Escherichia coli pathotypes-enteropathogenic E. coli (EPEC) and enterohaemorrhagic E. coli (EHEC)-have a conserved type 3 secretion system which is essential for virulence. The T3SS is used to translocate between 25 and 50 bacterial proteins directly into the host cytosol where they manipulate a variety of host cell processes to establish a successful infection. In this chapter, we discuss effectors from EPEC/EHEC in the context of the host proteins and processes that they target-the actin cytoskeleton, small guanosine triphosphatases and innate immune signalling pathways that regulate inflammation and cell death. Many of these translocated proteins have been extensively characterised, which has helped obtain insights into the mechanisms of pathogenesis of these bacteria and also understand the host pathways they target in more detail. With increasing knowledge of the positive and negative regulation of host signalling pathways by different effectors, a future challenge is to investigate how the specific effector repertoire of each strain cooperates over the course of an infection

An intra-bacterial activity for a T3SS effector

Many Gram-negative bacterial pathogens interact with mammalian cells by using type III secretion systems (T3SS) to inject virulence proteins into host cells. A subset of these injected protein 'effectors' are enzymes that inhibit the function of host proteins by catalyzing the addition of unusual post-translational modifications. The E. coli and Citrobacter rodentium NleB effectors, as well as the Salmonella enterica SseK effectors are glycosyltransferases that modify host protein substrates with N-acetyl glucosamine (GlcNAc) on arginine residues. This post-translational modification disrupts the normal functioning of host immune response proteins. T3SS effectors are thought to be inactive within the bacterium and fold into their active conformations after they are injected, due to the activity of chaperones that keep the effectors in a structural state permissive for secretion. While performing mass spectrometry experiments to identify glycosylation substrates of NleB orthologs, we unexpectedly observed that the bacterial glutathione synthetase (GshB) is glycosylated by NleB on arginine residue R256. NleB-mediated glycosylation of GshB resulted in enhanced GshB activity, leading to an increase in glutathione production, and promoted C. rodentium survival in oxidative stress conditions. These data represent, to our knowledge, the first intra-bacterial activity for a T3SS effector and show that arginine-GlcNAcylation, once thought to be restricted to host cell compartments, also plays an important role in regulating bacterial physiology

Arginine glycosylation enhances methylglyoxal detoxification

Type III secretion system effector proteins have primarily been characterized for their interactions with host cell proteins and their ability to disrupt host signaling pathways. We are testing the hypothesis that some effectors are active within the bacterium, where they modulate bacterial signal transduction and physiology. We previously determined that the Citrobacter rodentium effector NleB possesses an intra-bacterial glycosyltransferase activity that increases glutathione synthetase activity to protect the bacterium from oxidative stress. Here we investigated the potential intra-bacterial activities of NleB orthologs in Salmonella enterica and found that SseK1 and SseK3 mediate resistance to methylglyoxal. SseK1 glycosylates specific arginine residues on four proteins involved in methylglyoxal detoxification, namely GloA (R9), GloB (R190), GloC (R160), and YajL (R149). SseK1-mediated Arg-glycosylation of these four proteins significantly enhances their catalytic activity, thus providing another important example of the intra-bacterial activities of type three secretion system effector proteins. These data are also the first demonstration that a Salmonella T3SS effector is active within the bacterium

Stabilization of Soft Soil by a Sustainable Binder Comprises Ground Granulated Blast Slag (GGBS) and Cement Kiln Dust (CKD)

Due to its significant deficiencies such as low permeability, low bearing and shear strength, and excessive compressibility, soft soil is one of the most problematic types of soil in civil engineering and soil stabilization can be considered a suitable technique for pavements. This study investigates the use of ground granulated blast slag (GGBS) and cement kiln dust (CKD) as stabilizers for soft soil. Thus, this study involves two optimization stages; in the first stage, GGBS was incorporated into 0%, 3%, 6%, 9%, and 12% by the weight of cement to obtain the optimal percentage, which was 6%. Then, the optimal GGBS was blended with CKD in a binary system at 0%, 25%, 50%, 75%, and 100% by the dry weight of the soil. The testing program used in this paper was Atterberg limits with compaction parameters to investigate the physical properties and unconfined compressive strength (USC) at 7 and 28 days to examine the mechanical characteristics. In addition, the microstructures of the soil specimens were tested at 7 and 28 days using scanning electron microscopy (SEM). The findings reveal that the binary system enhanced the physical and mechanical properties of the soft soil. The optimum binder achieved in this study was 6% (25% GGBS and 75% CKD), which generates an increase in strength of about 3.3 times in 7 days, and of 5.5 times in 28 days in comparison to the untreated soil. The enhancement was attributed to the formation of the hydration products as approved by SEM. Consequently, in the case of soft subgrade soils, this technique can increase the pavement’s bearing capacity and performance

Stabilization of Soft Soil by a Sustainable Binder Comprises Ground Granulated Blast Slag (GGBS) and Cement Kiln Dust (CKD)

Due to its significant deficiencies such as low permeability, low bearing and shear strength, and excessive compressibility, soft soil is one of the most problematic types of soil in civil engineering and soil stabilization can be considered a suitable technique for pavements. This study investigates the use of ground granulated blast slag (GGBS) and cement kiln dust (CKD) as stabilizers for soft soil. Thus, this study involves two optimization stages; in the first stage, GGBS was incorporated into 0%, 3%, 6%, 9%, and 12% by the weight of cement to obtain the optimal percentage, which was 6%. Then, the optimal GGBS was blended with CKD in a binary system at 0%, 25%, 50%, 75%, and 100% by the dry weight of the soil. The testing program used in this paper was Atterberg limits with compaction parameters to investigate the physical properties and unconfined compressive strength (USC) at 7 and 28 days to examine the mechanical characteristics. In addition, the microstructures of the soil specimens were tested at 7 and 28 days using scanning electron microscopy (SEM). The findings reveal that the binary system enhanced the physical and mechanical properties of the soft soil. The optimum binder achieved in this study was 6% (25% GGBS and 75% CKD), which generates an increase in strength of about 3.3 times in 7 days, and of 5.5 times in 28 days in comparison to the untreated soil. The enhancement was attributed to the formation of the hydration products as approved by SEM. Consequently, in the case of soft subgrade soils, this technique can increase the pavement&rsquo;s bearing capacity and performance

NleB/SseK effectors from Citrobacter rodentium, Escherichia coli, and Salmonella enterica display distinct differences in host substrate specificity

Many Gram-negative bacterial pathogens use a syringe-like apparatus called a type III secretion system to inject virulence factors into host cells. Some of these effectors are enzymes that modify host proteins to subvert their normal functions. NleB is a glycosyltransferase that modifies host proteins with N-acetyl-D-glucosamine to inhibit antibacterial and inflammatory host responses. NleB is conserved among the attaching/effacing pathogens enterohemorrhagic Escherichia coli (EHEC), enteropathogenic E. coli (EPEC), and Citrobacter rodentium. Moreover, Salmonella enterica strains encode up to three NleB orthologs named SseK1, SseK2, and SseK3. However, there are conflicting reports regarding the activities and host protein targets among the NleB/SseK orthologs. Therefore, here we performed in vitro glycosylation assays and cell culture experiments to compare the activities and substrate specificities of these effectors. SseK1, SseK3, EHEC NleB1, EPEC NleB1, and C. rodentium NleB blocked TNF-mediated NF-B pathway activation, whereas SseK2 and NleB2 did not. C. rodentium NleB, EHEC NleB1, and SseK1 glycosylated host GAPDH. C. rodentium NleB, EHEC NleB1, EPEC NleB1, and SseK2 glycosylated the FADD (Fas-associated death domain protein). SseK3 and NleB2 were not active against either substrate. We also found that EHEC NleB1 glycosylated two GAPDH arginine residues, Arg and Arg, and that these two residues were essential for GAPDH-mediated activation of TNF receptor-associated factor 2 ubiquitination. These results provide evidence that members of this highly conserved family of bacterial virulence effectors target different host protein substrates and exhibit distinct cellular modes of action to suppress host responses.Peer Reviewe