201 research outputs found
Effects of Allelic Variation in Glutenin Subunits and Gliadins on Baking-Quality in Near-isogenic Lines of Common Wheat cv. Longmai 19
Two lines, L-19-613 and L-19-626, were produced from the common wheat cultivar Longmai 19 (L-19) by six consecutive backcrosses using biochemical marker-assisted selection. L-19 (Glu-D1a, Glu-A3c/Gli-A1?; Gli-A1? is a gene coding for unnamed gliadin) and L-19-613 (Glu-D1d, Glu-A3c/Gli-A1?) formed a set of near-isogenic lines (NILs) for HMW-GS, while L-19-613 and L-19-626 (Glu-D1d, Glu-A3e/Gli-A1m) constituted another set of NILs for the LMW-GS/gliadins. The three L-19 NILs were grown in the wheat breeding nursery in 2007 and 2008. The field experiments were designed using the three-column contrast arrangement method with four replicates. The three lines were ranked as follows for measurements of gluten strength, which was determined by the gluten index, Zeleny sedimentation, the stability and breakdown time of the farinogram, the maximum resistance and area of the extensogram, and the P andWvalues of the alveogram: L-19-613 > L-19-626 > L-19. The parameters listed above were significantly different between lines at the 0.05 or 0.01 level. The Glu-D1 and Glu-A3/Gli-A1 loci had additive effects on the gluten index, Zeleny sedimentation, stability, breakdown time, maximum resistance, area, P and W values. Although genetic variation at the Glu-A3/Gli-A1 locus had a great influence on wheat quality, the genetic difference between Glu-D1d and Glu-D1a at the Glu-D1 locus was much larger than that of Glu-A3c/Gli-A1? and Glu-A3e/Gli-A1m at the Glu-A3/Gli-A1 locus. Glu-D1d had negative effects on the extensibility and the L value compared with Glu-D1a. In contrast, Glu-A3c/Gli-A1? had a positive effect on these traits compared with Glu-A3e/Gli-A1m
Alternative splicing and genetic diversity of the white collar-1 (wc-1) gene in cereal Phaeosphaeria pathogens
The white collar-1 (wc-1) gene encodes an important light-responsive protein (wc-1) that maintains circadian clocks and controls numerous light-dependent reactions including sporulation in ascomycete fungi. The structure and expression of the wc-1 gene in wheat-biotype Phaeosphaeria nodorum (PN-w) was studied. It was shown that the full-size (3,353 bp in length) wc-1 gene in PN-w contained 4 introns in which introns 1 and 2 were flanked by GC-AG splice borders and were spliced constitutively. However, introns 3 and 4 of the wc-1 gene were alternatively spliced. As the result of alternative splicing (AS), six transcript variants were identified, encoding different lengths of deduced polypeptides (from 1,044 to 1,065aa). Ratios of the wc-1 gene transcript variants in the RNA population were the same in the sporulated and non-sporulated PN-w isolate Sn37-1 and the sporulated PN-w isolate S-79-1, grown under light/dark conditions. The AS of the wc-1 gene may control various light-dependent reactions in PN-w, which leads to diverse morphological, physiological and pathological characters for pathogen infection and spread. Based on the nucleotide and deduced amino acid sequences, the wc-1 gene in cereal Phaeosphaeria pathogens was diverse. It appeared that the deduced wc-1 polypeptide sequences of P. avenaria f. sp. avenaria (Paa), P. avenaria f. sp. triticea (Pat1 and Pat3) and barley biotype P. nodorum (PN-b) were more closely related than PN-w and Phaeosphaeeria sp. (P-rye) from Poland. Based on the wc-1 deduced polypeptide sequences, P. avenaria f. sp. triticea (Pat2) from foxtail barley (Hordeum jubatum L.) was evolutionary well separated from the other cereal Phaeosphaeria pathogens
A nitroreductase and glutathione responsive nanoplatform for integration of gene delivery and near-infrared fluorescence imaging
A novel platform rationally integrating indocyanine green analogues and an arginine-rich dendritic peptide with both nitroreductase (NTR) and glutathione (GSH) reduction responsive linkers was developed. This multifunctional platform can enable selective and efficient gene delivery and specific turn-on fluorescence imaging in tumors.Drug Delivery Technolog
Measurements of the Mass and Full-Width of the Meson
In a sample of 58 million events collected with the BES II detector,
the process J/ is observed in five different decay
channels: , , (with ), (with
) and . From a combined fit of all five
channels, we determine the mass and full-width of to be
MeV/ and
MeV/.Comment: 9 pages, 2 figures and 4 table. Submitted to Phys. Lett.
Partial Wave Analysis of
BES data on are presented. The
contribution peaks strongly near threshold. It is fitted with a
broad resonance with mass MeV, width MeV. A broad resonance peaking at 2020 MeV is also required
with width MeV. There is further evidence for a component
peaking at 2.55 GeV. The non- contribution is close to phase
space; it peaks at 2.6 GeV and is very different from .Comment: 15 pages, 6 figures, 1 table, Submitted to PL
Evidence of psi(3770) non-DD-bar Decay to J/psi pi+pi-
Evidence of decays to a non- final state is
observed. A total of \psi(3770) \to \PPJP events are
obtained from a data sample of 27.7 taken at center-of-mass
energies around 3.773 GeV using the BES-II detector at the BEPC. The branching
fraction is determined to be BF(\psi(3770) \to \PPJP)=(0.34\pm 0.14 \pm
0.09)%, corresponding to the partial width of \Gamma(\psi(3770) \to \PPJP) =
(80 \pm 33 \pm 23) keV.Comment: 8 pages, 7 figures, Submitted to Physics Letters
Measurement of Branching Ratios for Hadronic Decays
In a sample of 58 million events collected with the BES II detector,
the process J/ is observed in five decay channels: , , (with
), (with ) and . From
these signals, we determine ,
, ,
and .Comment: 8 pages, 1 figures and 4 table. Submitted to Phys. Lett.
Measurements of J/psi --> p \bar{p}
The process J/\psi --> p \bar{p} is studied using 57.7 X 10^6 J/\psi events
collected with the BESII detector at the Beijing Electron Positron Collider.
The branching ratio is determined to be Br(J/\psi --> p \bar{p})=(2.26 +- 0.01
+- 0.14) X 10^{-3}, and the angular distribution is well described by
\frac{dN}{d cos\theta_p}=1+\alpha\cos^2\theta_p with \alpha = 0.676 +- 0.036 +-
0.042, where \theta_p is the angle between the proton and beam directions. The
value of \alpha obtained is in good agreement with the predictions of
first-order QCD.Comment: 6 pages, 2 figures, RevTex4, Submitted to Phys.Lett.
A Study of J/psi-->gamma gamma V(rho,phi) Decays with the BESII Detector
Using a sample of events collected with the BESII
detector, radiative decays , where or ,
are studied. A resonance around 1420 MeV/c (X(1424)) is observed in the
mass spectrum. Its mass and width are measured to be MeV/c and MeV/c,
respectively, and its branching ratio is determined to be . A
search for yields a 95% C.L. upper limit .Comment: 10 pages, 5 figures, submitted to PL
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