Experiences and perceptions of abused Turkish women regarding violence against women

Bahadir Yilmaz, Emel/0000-0003-1785-3539WOS: 000463787900017PubMed: 30353446This study qualitatively explored abused Turkish women's experiences and perceptions regarding violence against women. The research sample comprised 30 women who were seen by the police and referred to the Family Counseling Center between 2 October 2012 and 30 August 2013 due to domestic violence. Study data were collected using a Demographic Information Datasheet and a Semi-Structured Interview Form. Qualitative data were gathered using face-to-face interviews, which were transcribed verbatim then analyzed and interpreted to determine themes using the conceptual framework. Qualitative analysis yielded five themes: (1) being subjected to all types of violence; (2) everything is an excuse for violence; (3) forgetting what it is like to be a woman; (4) having to continue the marriage against her will; (5) being forced to do some things involuntarily in order to reduce the violence. In the light of these results, it seems that multidisciplinary approaches are required in order to support women both psychologically and socially. Women need to be supported in order to determine their mental health problems and to get the required medical assistance

S100A16 promotes differentiation and contributes to a less aggressive tumor phenotype in oral squamous cell carcinoma

Background: Altered expression of S100A16 has been reported in human cancers, but its biological role in tumorigenesis is not fully understood. This study aimed to investigate the clinical significance and functional role of S100A16 in oral squamous cell carcinoma (OSCC) suppression. Methods: S100A16 mRNA and/or protein levels were examined by quantitative RT-PCR and immunohistochemistry in whole- and laser microdissected-specimens of normal human oral mucosa (NHOM, n = 65), oral dysplastic lesions (ODL, n = 21), OSCCs (n = 132) and positive cervical nodes (n = 17). S100A16 protein expression in OSCC was examined for correlations with clinicopathological variables and patient survival. S100A16 was over-expressed and knocked-down in OSCC-derived (CaLH3 and H357) cells by employing retroviral constructs to investigate its effects on cell proliferation, sphere formation and three dimensional (3D)-organotypic invasive abilities in vitro and tumorigenesis in a mouse xenograft model. Results: Both S100A16 mRNA and protein levels were found to be progressively down-regulated from NHOM to ODL and OSCC. Low S100A16 protein levels in OSCC significantly correlated with reduced 10-year overall survival and poor tumor differentiation. Analysis of two external OSCC microarray datasets showed a positive correlation between the mRNA expression levels of S100A16 and keratinocyte differentiation markers. CaLH3 and H357 cell fractions enriched for differentiated cells either by lack of adherence to collagen IV or FACS sorting for low p75NTR expression expressed significantly higher S100A16 mRNA levels than the subpopulations enriched for less differentiated cells. Corroborating these findings, retroviral mediated S100A16 over-expression and knock-down in CaLH3 and H357 cells led to respective up- and down-regulation of differentiation markers. In vitro functional studies showed significant reduction in cell proliferation, sphere formation and 3D-invasive abilities of CaLH3 and H357 cells upon S100A16 over-expression. These functional effects were associated with concomitant down-regulation of self-renewal (Bmi-1 and Oct 4A) and invasion related (MMP1 and MMP9) molecules. S100A16 over-expression also suppressed tumorigenesis of H357 cells in a mouse xenograft model and the resulting tumor xenografts displayed features/expression of increased differentiation and reduced proliferation/self-renewal. Conclusions: These results indicate that S100A16 is a differentiation promoting protein and might function as a tumor suppressor in OSCC