Genotyping of Sarawak rice cultivars using microsatellite markers

Genetic diversity of 53 Sarawak rice cultivars, originating from Southern Sarawak, was assessed using 54 microsatellite markers. Initial polymorphism detection was conducted using 54 primer pairs distributed on 12 rice chromosomes. Polymorphic markers were chosen from the initial screening results in order to obtain microsatellite marker panels that can differentiate the rice cultivars undertaken in the study. The chosen microsatellite marker panel consisted of RM1, RM240, RM489, RM252, RM413, RM204, RM11, RM404, RM316, RM271, RM206, and RM19, with one representative from each chromosome. A total of 43 alleles were detected with an average of 3.58 alleles per locus. The polymorphism information content (PIC) values obtained from the microsatellite marker panels ranged from 0.306 to 0.730, with an average of 0.622. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendrogram (r = 0.789) revealed 2 major groups with 6 sub-clusters and the wide range of similarity values (0.24-1.0) obtained showed a high degree of diversity among the cultivars. The results suggest microsatellite markers as a useful tool for the estimation of genetic diversity and cultivar differentiation and present invaluable genetic information for future breeding and association mapping efforts

Development of aircraft electric starter-generator system based-on active rectification technology

More-electric aircraft (MEA) has become a dominant trend for modern aircraft. On-board MEA, many functions, which are conventionally driven by pneumatic and hydraulic power, are replaced with electrical subsystems. Starting aircraft engines with an electrical motor instead of using pneumatic power from the auxiliary power unit (APU) is one of the major characteristics of future aircraft. This paper presents the development of a novel electric starter-generator system for aircraft applications. The paper describes the main achievements of the project within the key areas including electric machines, power electronic converters, thermal management and overall system control design. The developed prototype has been tested successfully and the test results are presented in this paper