Creating new perspectives on work-home interface : a cross-cultural comparison of Malta, Serbia and the Netherlands

Cultural context plays an important role in the way employees combine work and home duties. Insights in the role of cultural context are highly relevant for setting up effective interventions and policies to reduce negative work home interface and improve health and well-being of individuals. Data for the studies in this thesis were collected in Malta, Serbia and The Netherlands. Besides work and home characteristics, such as work demands and family situation, cultural context plays an important role. The role of culture is impacting the negotiation of combining work and home demands, and this negotiation is intertwined with the cultural norms and expectations, gender and age. Different aspects appear to be relevant in different cultures. For example, colleague support is important in the Netherlands, and in other contexts it is not. Convergence of policies at EU level will not contribute to better work-home interface. A culturally sensitive approach in policies and interventions is called for

Industrial Plant Layout Analyzing Based on SNA

Social network analysis (SNA) is a widely studied research topics, which has been increasingly being applied for solving different kind of problems, including industrial manufacturing ones. This paper focuses on the application of SNA on an industrial plant layout problem. The study aims at analyzing the importance of using SNA techniques to analyze important relations between entities in a manufacturing environment, such as jobs and resources in the context of industrial plant layout analysis. The study carried out enabled to obtain relevant results for the identification of relations among these entities for supporting to establish an appropriate plant layout for producing the jobs.This work has been supported by COMPETE: POCI-01-0145-FEDER-007043 and FCT - The Foundation for Science and Technology within the Project Scope: UID/CEC/00319/2013.info:eu-repo/semantics/publishedVersio

Analysing critical success factors for supporting online shopping

Copying or distributing in print or electronic forms without written permission of IGI Global is prohibited. Online shopping is nowadays is a highly frequent action but there are several critical factors that have to be considered for enabling websites and platforms to be able to offer all necessary requisites for guaranteeing user friendly, secure and also enjoyable shopping experiences to clients, offering them exactly what they expect to buy, and quickly find, among a huge offer available online. In this paper, a set of considered critical success factors are analysed on a set of top ranked websites, about luxurious furniture, to understand to what extent these critical factors are satisfied. The results can be taken into consideration for implementing a successful business through e-commerce from herein analysed perspectives.(undefined)info:eu-repo/semantics/publishedVersio

Effects of two common polymorphisms in the 3' untranslated regions of estrogen receptor β on mRNA stability and translatability

Estrogen signaling is mediated by estrogen receptors (ERs), ERα and ERβ. Aberrant estrogen signaling is involved in breast cancer development. ERα is one of the key biomarkers for diagnosis and treatment of breast cancer. Unlike ERα, ERβ is still not introduced as a marker for diagnosis and established as a target of therapy. Numerous studies suggest antiproliferative effects of ERβ, however its role remains to be fully explored. Albeit important, ERα is not a perfect marker, and some aspects of ERα function are still unclear. This thesis aims to characterize distinct molecular facets of ER action relevant for breast cancer and provide valuable information for ER-based diagnosis and treatment design. In PAPER I, we analyzed the functionality of two common single nucleotide polymorphisms in the 3’ untranslated regions of ERβ, rs4986938 and rs928554, which have been extensively investigated for association with various diseases. A significant difference in allelic expression was observed for rs4986938 in breast tumor samples from heterozygous individuals. However, no difference in mRNA stability or translatability between the alleles was observed. In PAPER II, we provided a more comprehensive understanding of ERβ function independent of ERα. A global gene expression analysis in a HEK293/ERβ cell model identified a set of ERβ-regulated genes. Gene Ontology (GO) analysis showed that they are involved in cell-cell signaling, morphogenesis and cell proliferation. Moreover, ERβ expression resulted in a significant decrease in cell proliferation. In PAPER III, using the human breast cancer MCF-7/ERβ cell model, we demonstrated, for the first time, the binding of ERα/β heterodimers to various DNA-binding regions in intact chromatin. In PAPER IV, we investigated a potential cross-talk between estrogen signaling and DNA methylation by identifying their common target genes in MCF-7 cells. Gene expression profiling identified around 150 genes regulated by both 17β- estradiol (E2) and a hypomethylating agent 5-aza-2’-deoxycytidine. Based on GO analysis, CpG island prediction analysis and previously reported ER binding regions, we selected six genes for further analysis. We identified BTG3 and FHL2 as direct target genes of both pathways. However, our data did not support a direct molecular interplay of mediators of estrogen and epigenetic signaling at promoters of regulated genes. In PAPER V, we further explored the interactions between estrogen signaling and DNA methylation, with focus on DNA methyltransferases (DNMT1, DNMT3a and DNMT3b). E2, via ERα, up-regulated DNMT1 and down-regulated DNMT3a and DNMT3b mRNA expression. Furthermore, DNMT3b interacted with ERα. siRNA-mediated DNMT3b depletion increased the expression of two genes, CDKN1A and FHL2. We proposed that the molecular mechanism underlying regulation of FHL2 and CDKN1A gene expression involves interplay of DNMT3b and ERα. In conclusion, the studies presented in this thesis contribute to the knowledge of ERβ function, and give additional insight into the cross-talk mechanisms underlying ERα signaling with ERβ and with DNA methylation pathways

Analysing the correlation between social network analysis measures and performance of students in social network-based engineering education

Social network-based engineering education (SNEE) is designed and implemented as a model of Education 3.0 paradigm. SNEE represents a new learning methodology, which is based on the concept of social networks and represents an extended model of project-led education. The concept of social networks was applied in the real-life experiment, considering two different dimensions: (1) to organize the education process as a social network-based process; and (2) to analyze the students' interactions in the context of evaluation of the students learning performance. The objective of this paper is to present a new model for students evaluation based on their behavior during the course and its validation in comparison with the traditional model of students' evaluation. The validation of the new evaluation model is made through an analysis of the correlation between social network analysis measures (degree centrality, closeness centrality, betweenness centrality, eigenvector centrality, and average tie strength) and the grades obtained by students (grades for quality of work, grades for volume of work, grades for diversity of work, and final grades) in a social network-based engineering education. The main finding is that the obtained correlation results can be used to make the process of the students' performance evaluation based on students interactions (behavior) analysis, to make the evaluation partially automatic, increasing the objectivity and productivity of teachers and allowing a more scalable process of evaluation. The results also contribute to the behavioural theory of learning performance evaluation. More specific findings related to the correlation analysis are: (1) the more different interactions a student had (degree centrality) and the more frequently the student was between the interaction paths of other students (betweenness centrality), the better was the quality of the work; (2) all five social network measures had a positive and strong correlation with the grade for volume of work and with the final graThe authors wish to acknowledge the support of the Fundacao para a Ciencia e Tecnologia (FCT), Portugal, through the Grants "Projeto Estrategico-UI 252-2011-2012'' reference PEst-OE/EME/UI0252/2011, "Ph.D. Scholarship Grant'' reference SFRH/BD/85672/2012, and the support of Parallel Planes Lda.info:eu-repo/semantics/publishedVersio

Estrogen mediated-activation of miR-191/425 cluster modulates tumorigenicity of breast cancer cells depending on estrogen receptor status.

MicroRNAs (miRNAs), single-stranded non-coding RNAs, influence myriad biological processes that can contribute to cancer. Although tumor-suppressive and oncogenic functions have been characterized for some miRNAs, the majority of microRNAs have not been investigated for their ability to promote and modulate tumorigenesis. Here, we established that the miR-191/425 cluster is transcriptionally dependent on the host gene, DALRD3, and that the hormone 17β-estradiol (estrogen or E2) controls expression of both miR-191/425 and DALRD3. MiR-191/425 locus characterization revealed that the recruitment of estrogen receptor α (ERα) to the regulatory region of the miR-191/425-DALRD3 unit resulted in the accumulation of miR-191 and miR-425 and subsequent decrease in DALRD3 expression levels. We demonstrated that miR-191 protects ERα positive breast cancer cells from hormone starvation-induced apoptosis through the suppression of tumor-suppressor EGR1. Furthermore, enforced expression of the miR-191/425 cluster in aggressive breast cancer cells altered global gene expression profiles and enabled us to identify important tumor promoting genes, including SATB1, CCND2, and FSCN1, as targets of miR-191 and miR-425. Finally, in vitro and in vivo experiments demonstrated that miR-191 and miR-425 reduced proliferation, impaired tumorigenesis and metastasis, and increased expression of epithelial markers in aggressive breast cancer cells. Our data provide compelling evidence for the transcriptional regulation of the miR-191/425 cluster and for its context-specific biological determinants in breast cancers. Importantly, we demonstrated that the miR-191/425 cluster, by reducing the expression of an extensive network of genes, has a fundamental impact on cancer initiation and progression of breast cancer cells

Long-term results of laparoscopic gastric sleeve resection due to morbid obesity and metabolic syndrome

© 2018, Serbia Medical Society. All rights reserved. Introduction The aim of this paper was to presents long-term results of a laparoscopic gastric sleeve resection in a “super super” obese patient and a follow-up period of eight years. Case outline A patient with body mass index of 70 kg/m2 and Stage 3 obesity according to the King’s Obesity Staging Criteria, with metabolic syndrome and cardiovascular risk of over 20%, and a pronounced severe obstructive sleep apnea, underwent a laparoscopic gastric sleeve resection. After two years, the patient reached body mass index of 28.4 kg/m2 and eight years after the surgery has a body mass index of 34.3 kg/m2, and the percentage of excess body mass index loss of 79.3%. According to the King’s Obesity Staging Criteria, he falls under Stage 0. Conclusion Laparoscopic gastric sleeve resection may be performed as a stand-alone procedure in “super super” obese patients, with excellent long-term results