Motility and Viability of Friesian Holstein Spermatozoa in Three Different Extender Stored at 5oc

The aims of this study was to compare Tris egg yolk and Citrate egg yolk extender andsupplementation of fructose on citrate egg yolk on the quality of Friesian Holstein (FH) bull semenstored at 5 oC. Semen was collected from 5 FH bulls using an artificial vagina. The semen wereevaluated macroscopic and microscopically. The semen divided into three tubes and extended with Trisegg yolk (TEY), Citrate egg yolk (CEY) or Citrate fructose egg yolk (CFEY). Extended semen wasstored at 5 oC and evaluate daily for sperm motility and viability. There was no significant differences(P>0.05) on the sperm viability among three extender, for every time observation during 144 hours ofstorage. This similar finding found on the sperm motility in all extender for 48 hours of storage. Thesperm motility in TEY demonstrated significantly greater (P<0.05) than in CFEY and CEY extender at72 to 120 hours storage. In the end of storage, sperm motility in TEY (35.2 ± 4.1%) and CFEY (33.5 ±2.71%) extender statistically indicated no significant different, and both were greater than CEY. Inconclusion, CFEY support the sperm motility as good as TEY of FH bull

Intensitas Dan Waktu Estrus Pada Berbagai Paritas Induk Sapi Perah Fries Holland Pasca Partus

Profile Intensity And Estrus Time In Various Parity Fries Holland Dairy Cattle Postpartum. Parity is a period in the reproductive cycle of cattle with an indication of the amount of carrier confinement livestock. Feliciano, et al, (2003) states that the parity is classified into three parts, namely: (1) nuliparous , (2) primiparous and (3) pluriparous / multiparous.This study used one-way classification design with the observed 90 cows post-partum is composed of 30 first parity, 30 second parity and 30 third parity. Time of estrus observation data were analyzed with General Linear Model (GLM) followed by Duncan Multiple Range Test (DMRT).This study used one-way classification design with the observed 90 cows post-partum was composed of 30 first parity, 30 second parity and 30 third parity. Data observation of estrus time were analyzed with General Linear Model (GLM) followed by Duncan Multiple Range Test (DMRT).It could be concluded that the parity I, II and III with two estrus intensity (+ +), each for 63.3%, 46.7% and 73.4% and third estrus intensity (+ + +) respectively amounted to 36.6%, 53.3% and 26.6%. While the second estrus postpartum in various parity indicates a difference in means that the parity gives effect to the second estrus postpartum. While the second estrus postpartum in various parity indicated a significance difference that the parity gave effect to the second estrus postpartum

Occurrence of Sperm Abnormality of Beef Cattle at Several Artificial Insemination Centers in Indonesia

In the most species studied sperm abnormalities have long been associated with male infertility and sterility. This study evaluated the sperm morphology (normality and abnormality) of beef cattle at several Artificial Insemination centers in Indonesia. Total of 142 bulls were used in this study; an ejaculate from each bull was examined. A drop of semen was placed on 3-4 glass slides, and smears were prepared and air-dried. The smears were stained with carbolfluchsin-eosin (Williams stain). Types of morphological abnormalities were recorded from 500 cells on each sample. It was recorded that 77.46% samples had low primary sperm abnormalities (10%) was found in 5.63% samples.  Pear shaped was the most frequently type of abnormality found on examined samples (2.24 ± 2.94%); while double head was the lowest (0.01 ± 0.04%). (Animal Production 12(1): 44-49 (2010) Key words : sperm abnormality, beef catlle, artificial insemination cente

Occurrence of Sperm Abnormality of Beef Cattle at Several Artificial Insemination Centers in Indonesia

In the most species studied sperm abnormalities have long been associated with male infertility and sterility. This study evaluated the sperm morphology (normality and abnormality) of beef cattle at several Artificial Insemination centers in Indonesia. Total of 142 bulls were used in this study; an ejaculate from each bull was examined. A drop of semen was placed on 3-4 glass slides, and smears were prepared and air-dried. The smears were stained with carbolfluchsin-eosin (Williams stain). Types of morphological abnormalities were recorded from 500 cells on each sample. It was recorded that 77.46% samples had low primary sperm abnormalities (10%) was found in 5.63% samples. Pear shaped was the most frequently type of abnormality found on examined samples (2.24 ± 2.94%); while double head was the lowest (0.01 ± 0.04%). (Animal Production 12(1): 44-49 (2010

Pembekuan Semen Lele Dumbo (Clarias Gariepinus Burchell 1822) Sebagai Model Kriopreservasi Semen Ikan [Freezing of African Catfish Semen (Clarias Gariepinus Burchell 1822) as a Model of Cryopreservation Fish Semen]

Efek empat jenis pengencer dan empat konsentrasi dimetil sulfoksida (DMSO) (5%, 10%, 15% dan 20%) dari motilitas spermatozoa ikan lele dumbo dievaluasi setelah penyimpanan pada suhu beku. Upaya kriopreservasi semen lele dumbo terhadap 16 kombinasi perlakuan yang terdiri atas beberapa tahap, yaitu: persiapan pengenceran semen lele dumbo; pencampuran dengan bahan pengencer DMSO; pengepakan semen di dalam straw 0,3 ml; equilibrasi pada suhu lemari es pada suhu 4oC selama 30 menit; semen lele dumbo dibekukan di atas uap nitrogen cair dengan tinggi 6,5 cm selama 10 menit dan selanjutnya disimpan dalam wadah nitrogen cair (-196oC) untuk dianalisis lanjut guna melihat postthawing motility (PTM). Hasil analisis PTM, terhadap tingkat motilitas spermatozoa lele dumbo, terlihat bahwa nilai tertinggi pada perlakuan PiD15 (45,7±4,3%) dan terendah pada perlakuan P2D20 (14,5±13,2%). Pengencer terbaik ada-lah pengencer yang mengandung NaCl, KCl, CaCl2, dan NaHCO3. Konsentrasi terbaik DMSO adalah konsentrasi DMSO 15%. Sementara interaksi terbaik antara pengencer dengan konsentrasi DMSO adalah PiD15 perlakuan yang mengandung NaCl, KCl, CaCl2, dan NaHCO3 dengan kombinasi konsentrasi DMSO 15%. Kesimpulan, upaya kriopreservasi sperma lele dumbo dapat menggunakan pengencer yang mengandung NaCl, KCl, CaCl2, dan NaHCO3 dengan kombinasi konsentrasi DMSO 15%

Sperm Morphological Assessments of Friesian Holstein Bull Semen Collected From Three Artificial Insemination Centers in Indonesia

Morphologically abnormal sperm in semen has been associated with the sub fertility and sterilityfor many years. This study assessed the sperm morphology of Friesian Holstein bull semen which wascollected from three Artificial Insemination centers in Indonesia. Total of 22 bulls were used in thisstudy; an ejaculate from each bull was examined. Three to four glass slides were prepared for each bullsample; a drop of semen was placed on each glass slide, smeared, and air-dried. The smeared sampleswere stained with carbolfuchsin-eosin (Williams stain). Morphological abnormality types were recordedfrom total of 500 spermatozoa. Results demonstrated that all 22 bulls had low sperm abnormality(<10%). Pearshaped was the most frequently type of sperm abnormality found in the samples(0.81±0.93%); while detached head was the lowest (0.01±0.04%)

The viability of fresh and extended semen of stallion with different sperm concentration in Dimitropoulos-modified extender

The objective of the experiment was to study the motility and viability of spermatozoa of fresh semen, and the quality of extended semen with different sperm concentration in Dimitropoulos-modified extender. Semen was collected using artificial vagina from three 4-8 year old stallions (different breed). Semen characteristics and quality was evaluated macro- and microscopically. For longevity evaluation, semen was stored at room and chilled temperature, and was evaluated for motility and viability every 3 h. Prior extension, semen was centrifugated at 3000 rpm for 20 minutes. The condensed sperm was re-suspended in Dimitropoulos (DV) supplemented with 50 mM fructose with the concentration of 200, 100 and 50 x 106 spz/mL. All samples were stored at room and chilled temperature, and was evaluated for motility and viability every 3 h and 12 h for room and chilled temperature. Results of the experiments indicated that fresh semen characteristics was fairly good. For longevity evaluation, semen with motility of 48.33 and 10.42% was observed at 3 h and 12 h after the onset of storage. The extended-semen with 50 x 106 spz/mL showed significantly higher in term of motility and viability (P.05) than that with 200 x 106 spz/mL, but for that of 100 x 106 spz/mL. It is recommended that sperm concentration should be 50 x 106 spz/ml for a long period storage with reasonable good quality. Key words: Stallion, Semen, Sperm Concentration, Dimitropoulo

Pola Distribusi Mitokondria Sel-sel Trofoblas Blastosis Mencit (Mus Muculus Albinus) Dan Pengaruhnya Terhadap Kegagalan Hatching Dan Implantasi

Implantation is the most critical stage in the establishment of pregnancy. In mammals, it has been estimated that between 25% and 60% of conceptuses are lost before or at the time of implantation. The objectives of this study were to investigate the ability of blastocyst hatching and attachment of trophoblast cells in in vitro, the outgrowth and differentiation of trophoblast cells in in vitro culture of hatched and non hatched blastocyst, the activity of mitochondria NADH-CoQ reductase and the pattern of mitochondrial distribution. Blastocysts were collected from mice cornua utery at day-4 of pregnancy and were divided into 3 groups: blastocysts undergo hatching within 24 hours, 48 hours and non hatching. Embryos were cultured in DMEM medium in 5% CO2 incubator at 37°C for 10 days. The trophoblasts monolayer were processed for Giemsa staining and histochemistry analysis of NADH-tetrazolium reductase activity. The outgrowth of trophoblast cells were measured using eyespiece micrometer. The results showed the ability of blastocyst hatching in in vitro were different. The trophoblast outgrowth diameter of 24 h hatched blastocyst was significantly different with the 48 h hatched and non hatched blastocyst. The activity of NADH-CoQ reductase of 24 h and 48 h hatched blastocysts showed higher intensity than the non hatched blastocysts (P<0,05) and the distribution of mitochondrial in trophoblast cell cytoplasma of 24 h and 48 h hatched blastocyst were homogen around nucleus whereas thoes of non hatched blastocyst were cluster and heterogen. In conclusion, in in vitro study the failure of blastocysts hatching and implantation was due to the failure of outgrowth and differentiation of the trofoblast cells and the impairment of NADH-CoQ reductase activity in complex I mitochondria to produce energy and the pattern of mitochondrial distribution

Deer Frozen Semen Quality in Tris Sucrose and Tris Glucose Extender with Different Glycerol Concentrations

In order to improve Timor deer (Cervus timorensis) frozen semen quality, the influence of sugar and glycerol concentration on semen characteristics of sperm was investigated. The semen was collected from five sexually mature Timor deer using an electroejaculator. The semen was evaluated and divided into six equal tubes and diluted with Tris sucrose glycerol 10% (TSG10); Tris sucrose glycerol 12% (TSG12); Tris sucrose glycerol 14% (TSG14); Tris glucose glycerol 10% (TGG10); Tris glucose glycerol 12% (TGG12); and Tris glucose glycerol 14% (TGG14). The diluted semen was packed in 0.3 ml minitub straw, equilibrated at 5 oC for 4 hours and frozen on liquid nitrogen vapor for 10 minutes. The total of forward motility, viability, acrosome integrity and membrane integrity were assessed in fresh, after equilibration and after thawing. Results demonstrated that the percentage of sperm motility in TSG10was higher (P (63.93±7.23%). The sperm in TSG10 and TSG14 extender were superior in acrosome as well as in membrane integrity. It was concluded that Tris Sucrose with 10% glycerol protected Timor deer sperm better than other combinations