26 research outputs found
A Dynamic Vocabulary Speech Recognizer Using Real-Time, Associative-Based Learning
Conventional speech recognizers employ a training phase during which many of their parameters are configured - including vocabulary selection, feature selection, and decision mechanism tailoring to these selections. After this stage during normal operation, these traditional recognizers do not significantly alter any of these parameters. Conversely this work draws heavily on high level human thought patterns and speech perception to outline a set of precepts to eliminate this training phase and instead opt to perform all its tasks during the normal operation. A feature space model is discussed to establish a set of necessary and sufficient conditions to guide real-time feature selection. Detailed implementation and preliminary results are also discussed. These results indicate that benefits of this approach can be seen in increased speech recognizer adaptability while still retaining competitive recognition rates in controlled environments. Thus this can accommodate such changes as varying vocabularies, class migration, and new speakers
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A Dynamic Vocabulary Speech Recognizer Using Real-Time, Associative-Based Learning By
I hereby declare that I am the sole author of this thesis. This is a true copy of the thesis, including any final revisions, as accepted by my examiners. I understand that my thesis may be made electronically available to the public. ii Conventional speech recognizers employ a training phase during which many of their parameters are configured- including vocabulary selection, feature selection, and decision mechanism tailoring to these selections. After this stage during normal operation, these traditional recognizers do not significantly alter any of these parameters. Conversely this work draws heavily on high level human thought patterns and speech perception to outline a set of precepts to eliminate this training phase and instead opt to perform all its tasks during the normal operation. A feature space model is discussed to establish a set of necessary and sufficient conditions to guide real-time feature selection. Detailed implementation and preliminary results are also discussed. These results indicate that benefits of this approach can be seen in increased speech recognizer adaptability while still retaining competitiv
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Synthetic and Biocatalytic Strategies for Natural Product Synthesis via ortho-Quinone Methide Intermediates
Organisms across all domains of life have developed elegant strategies to produce specialized metabolites, also referred to as natural products, for defense, structure, and communication. Natural products provide novel inspiration for pharmaceutical development given their potent biological activities and structural diversity. Recent advancements in DNA sequencing technology and the development of predictive bioinformatics tools have unveiled a vast collection of biosynthetic machinery responsible for generating this structural diversity that may be harnessed by synthetic chemists. Many of the enzymes encoded by biosynthetic genes catalyze chemical reactions that are difficult to replicate with the same selectivity by traditional synthetic methods. An example of this discrepancy is illustrated by the current enzymatic and chemical methods known to generate a highly reactive ortho-quinone methide (o-QM) intermediate. The utility of o-QMs in total synthesis has been hampered by complications that surround the preparation of their precursors, the harsh generation methods, and poor chemo-, regio-, and stereoselective control. In contrast, multiple enzymes have been reported to catalyze o-QM formation under mild conditions with remarkable selectivity. Chapter 1 of this dissertation examines several different enzymatic strategies developed to generate o-QMs. Chapter 2 describes progress towards the biomimetic total synthesis of (–)-chlorizidine A that aims to synthetically replicate the final biosynthetic intramolecular cyclization via an o-QM intermediate. Chapter 3 reports the discovery of tetrachlorizine, a novel tetrachlorinated marine natural product, and the identification of its associated biosynthetic gene cluster. Biochemical characterization of a pivotal flavin-dependent oxidase revealed a dehydrogenation reaction that proceeds via an unprecedented o-QM mechanism. This oxidase was then repurposed to generate an isolable o-QM, an extremely rare chemical motif. Chapter 4 investigates the structure-function relationships of two homologous flavin-dependent oxidases with divergent reaction selectivities and highlights their favorable biocatalytic properties. This chapter also discloses the first reported bacterial enzymes capable of performing oxidative cyclization reactions to produce cannabinoids