2,189 research outputs found
Alternate routes to the cell surface underpin insulin-regulated membrane trafficking of GLUT4
Insulin-stimulated delivery of glucose transporters (GLUT4) from specialized intracellular GLUT4 storage vesicles (GSVs) to the surface of fat and muscle cells is central to whole-body glucose. This translocation and subsequent internalization of GLUT4 back into intracellular stores transits numerous small membrane-bound compartments (internal GLUT4-containing vesicles; IGVs) including GSVs, but the function of these different compartments is not clear. Cellugyrin and sortilin define distinct populations of IGV; sortilin-positive IGVs represent GSVs, but the function of cellugyrin-containing IGVs is unknown. Here we demonstrate a role for cellugyrin in intracellular sequestration of GLUT4 in HeLa cells and have used a proximity ligation assay to follow changes in pairwise associations between cellugyrin, sortilin, GLUT4 and membrane trafficking machinery following insulin-stimulation of 3T3-L1 adipoctyes. Our data suggest that insulin stimulates traffic from cellugyrin- to sortilin- membranes, and that cellugyrin-IGVs provide an insulin-sensitive reservoir to replenish GSVs following insulin-stimulated exocytosis of GLUT4. Furthermore, our data support the existence of a pathway from cellugyrin-membranes to the surface of 3T3-L1 adipocytes that bypasses GSVs under basal conditions, and that insulin diverts traffic away from this into GSVs
Computerized system for translating a torch head
The system provides a constant travel speed along a contoured workpiece. It has a driven skate characterized by an elongated bed, with a pair of independently pivoted trucks connected to the bed for support. The trucks are mounted on a contoured track of arbitrary configuration in a mutually spaced relation. An axially extensible torch head manipulator arm is mounted on the bed of the carriage and projects perpendicular from the midportion. The torch head is mounted at its distal end. A real-time computerized control drive subsystem is used to advance the skate along the track of a variable rate for maintaining a constant speed for the torch head tip, and to position the torch axis relative to a preset angle to the workpiece
Unveiling the Mysteries of Aceh, Indonesia: Local and Global Intersections of Women\u27s Agency
Forces of globalization, local culture, and Islam continuously inform one another and dynamically manifest in cultures across the world. Scholars often assume that these influences may have distinct and independent effects. However, we argue that these global forces occur simultaneously and they may contradict or complement each other along a spectrum within Aceh, Indonesia. The manifestations and responses vary depending on the nature of the interactions of global and local factors. This spectrum represents various ways in which women negotiate identity and agency, specifically within the context of the implementation of Shari’ah Law. This research investigates the specific ways in which women’s identities influence and are influenced by the globalization of feminism, matrifocal traditions, and Islamic veiling practices in Aceh.
In the summer of 2012, the authors conducted field research in Aceh, Indonesia through interviews and observations. These included over 70 participants and 20 organizations which varied in formality and size. The interview participants include: activists, academicians, spiritual leaders, government officials, law enforcement agents, university students, and other community members from both rural and urban areas
Anomalous quantum confined Stark effects in stacked InAs/GaAs self-assembled quantum dots
Vertically stacked and coupled InAs/GaAs self-assembled quantum dots (SADs)
are predicted to exhibit a strong non-parabolic dependence of the interband
transition energy on the electric field, which is not encountered in single SAD
structures nor in other types of quantum structures. Our study based on an
eight-band strain-dependent Hamiltonian indicates that
this anomalous quantum confined Stark effect is caused by the three-dimensional
strain field distribution which influences drastically the hole states in the
stacked SAD structures.Comment: 4 pages, 4 figure
Tight-binding study of the influence of the strain on the electronic properties of InAs/GaAs quantum dots
We present an atomistic investigation of the influence of strain on the
electronic properties of quantum dots (QD's) within the empirical tight-binding (ETB) model with interactions up to 2nd nearest neighbors
and spin-orbit coupling. Results for the model system of capped pyramid-shaped
InAs QD's in GaAs, with supercells containing atoms are presented and
compared with previous empirical pseudopotential results. The good agreement
shows that ETB is a reliable alternative for an atomistic treatment. The strain
is incorporated through the atomistic valence force field model. The ETB
treatment allows for the effects of bond length and bond angle deviations from
the ideal InAs and GaAs zincblende structure to be selectively removed from the
electronic-structure calculation, giving quantitative information on the
importance of strain effects on the bound state energies and on the physical
origin of the spatial elongation of the wave functions. Effects of dot-dot
coupling have also been examined to determine the relative weight of both
strain field and wave function overlap.Comment: 22 pages, 7 figures, submitted to Phys. Rev. B (in press) In the
latest version, added Figs. 3 and 4, modified Fig. 5, Tables I and II,.and
added new reference
Theoretical interpretation of the experimental electronic structure of lens shaped, self-assembled InAs/GaAs quantum dots
We adopt an atomistic pseudopotential description of the electronic structure
of self-assembled, lens shaped InAs quantum dots within the ``linear
combination of bulk bands'' method. We present a detailed comparison with
experiment, including quantites such as the single particle electron and hole
energy level spacings, the excitonic band gap, the electron-electron, hole-hole
and electron hole Coulomb energies and the optical polarization anisotropy. We
find a generally good agreement, which is improved even further for a dot
composition where some Ga has diffused into the dots.Comment: 16 pages, 5 figures. Submitted to Physical Review
Mean Field Phase Diagram of SU(2)xSU(2) Lattice Higgs-Yukawa Model at Finite Lambda
The phase diagram of an SU(2)_L x SU(2)_R lattice Higgs-Yukawa model with
finite lambda is constructed using mean field theory. The phase diagram bears a
superficial resemblance to that for infinite lambda, however as lambda is
decreased the paramagnetic region shrinks in size. For small lambda the phase
transitions remain second order, and no new first order transitions are seen.Comment: 9 pages, 3 postscript figures, RevTex. To appear in PR
Hubble Space Telescope Evidence for an Intermediate-Mass Black Hole in the Globular Cluster M15: II. Kinematical Analysis and Dynamical Modeling
We analyze HST/STIS spectra (see Paper I) of the central region of the dense
globular cluster M15. We infer the velocities of 64 individual stars,
two-thirds of which have their velocity measured for the first time. This
triples the number of stars with measured velocities in the central 1 arcsec of
M15 and doubles the number in the central 2 arcsec. Combined with existing
ground-based data we obtain the radial profiles of the projected kinematical
quantities. The RMS velocity sigma_RMS rises to 14 km/s in the central few
arcsec, somewhat higher than the values of 10-12 km/s inferred previously from
ground-based data. To interpret the results we construct dynamical models based
on the Jeans equation, which imply that M15 must have a central concentration
of non-luminous material. If this is due to a single black hole, then its mass
is M_BH = (3.9 +/- 2.2) x 10^3 solar masses. This is consistent with the
relation between M_BH and sigma_RMS that has been established for galaxies.
Also, the existence of intermediate-mass black holes in globular clusters is
consistent with several scenarios for globular cluster evolution proposed in
the literature. Therefore, these results may have important implications for
our understanding of the evolution of globular clusters, the growth of black
holes, the connection between globular cluster and galaxy formation, and the
nature of the recently discovered `ultra-luminous' X-ray sources in nearby
galaxies. Instead of a single black hole, M15 could have a central
concentration of dark remnants (e.g., neutron stars) due to mass segregation.
However, the best-fitting Fokker-Planck models that have previously been
constructed for M15 do not predict a central mass concentration that is
sufficient to explain the observed kinematics.[ABRIDGED]Comment: 43 pages, LaTeX, with 14 PostScript figures. Astronomical Journal, in
press (Dec 2002). Please note that the results reported here are modified by
the Addendum available at astro-ph/0210158 (Astronomical Journal, in press,
Jan 2003). This second version submitted to astro-ph is identical to first,
with the exception of the preceeding remar
Bovine Follicular Dynamics, Oocyte Recovery,and Development of Oocytes Microinjected with a Green Fluorescent Protein Construct
The present study was carried out to 1) evaluate the viability of in vitro fertilized zygotes after microinjection of DNA, 2) assess the influence of oocyte quality upon the development rate of embryos when injected with DNA, and 3) determine the integration frequency of green fluorescent protein DNA into microinjected embryos. Oocytes were aspirated from ovaries of nine nonlactating Holsteins and were categorized into grades A, B, C, and D. At 16 h after in vitro fertilization, approximately half of the pronuclear stage presumptive zygotes were classified as having 1 pronucleus or 2 pronuclei, and they were microinjected with DNA constructs. A potential predictor of DNA integration frequency at d 10 was assessment of the incidence of green fluorescing embryos. The proportion of cleaved embryos that developed to morulae or blastocysts was not different between groups with 1 pronucleus injected (45%), 1 pronucleus uninjected (64%), or 2 pronuclei injected (49%). However, the development of morulae or blastocysts was higher in the group with 2 pronuclei uninjected (69%). The overall developmental score of green fluorescent protein-positive embryos was higher for grade A oocytes (1.3 &#;&#;0.1) than for grade B (0.8 &#; 0.1), C (0.6 &#;&#;0.1), or D (0.3 &#;&#;0.1) oocytes. The results show that production of transgenic bovine blastocysts can occur from the microinjection of a presumptive zygote having only one visible pronucleus. Initial oocyte quality is an important factor in selection of oocytes suitable for microinjection of DNA and for preimplantation development to produce bovine transgenic embryos
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