Diagnostic de trypanosoma evansi : mise en place, standardisation et évaluation d'outils de PCR et d'Ac-ELISA : application à une étude préliminaire chez le bovin laitier en Thaïlande

La trypanosomose à Trypanosoma evansi, ou Surra, est la trypanosomose la plus largement répartie à travers le monde et touchant le plus grand nombre d'espèces hôtes. Le Surra affecte très fortement l'élevage (bovins, buffles et porcs), et constitue parfois un frein important au développement agricole de certain pays. Les pertes économiques sont énormes bien que le plus souvent très mal estimées. Le besoin de connaissances approfondies sur ce fléau a entraîné la mise en place en Thaïlande d'un projet de recherche sur Trypanosoma evansi à visée régionale. L'initiation d'un tel projet nécessite la mise en place préliminaire d'outils diagnostiques performants. La mise en place d'un test Ac-ELISA pour bovins, la muse en place et l'évaluation comparative de différents couples d'amorces PCR et la standardisation de ces outils ont ainsi été réalisées dans le laboratoire de parasitologie de l'université de Kasetsart (Bangkok, Thaïlande). Ce travail rend également compte de l'application de ces outils à une étude préliminaire sur l'élevage laitier en Thaïlande

Editorial: Anthropogenic wildlife movements and infectious diseases: Health and conservation perspectives

EditorialS

Epidemiology and pathological progression of erythematous lip lesions in captive sun bears (Helarctos malayanus)

This study investigates the occurrence of erythematous lip lesions in a captive sun bear population in Cambodia, including the progression of cheilitis to squamous cell carcinoma, and the presence of Ursid gammaherpesvirus 1. Visual assessment conducted in 2015 and 2016 recorded the prevalence and severity of lesions. Opportunistic sampling for disease testing was conducted on a subset of 39 sun bears, with histopathological examination of lip and tongue biopsies and PCR testing of oral swabs and tissue biopsies collected during health examinations. Lip lesions were similarly prevalent in 2015 (66.0%) and 2016 (68.3%). Degradation of lip lesion severity was seen between 2015 and 2016, and the odds of having lip lesions, having more severe lip lesions, and having lip lesion degradation over time, all increased with age. Cheilitis was found in all lip lesion biopsies, with histological confirmation of squamous cell carcinoma in 64.5% of cases. Single biopsies frequently showed progression from dysplasia to neoplasia. Eighteen of 31 sun bears (58.1%) had at least one sample positive for Ursid gammaherpesvirus 1. The virus was detected in sun bears with and without lip lesions, however due to case selection being strongly biased towards those showing lip lesions it was not possible to test for association between Ursid gammaherpesvirus 1 and lip squamous cell carcinoma. Given gammaherpesviruses can play a role in cancer development under certain conditions in other species, we believe further investigation into Ursid gammaherpesvirus 1 as one of a number of possible co-factors in the progression of lip lesions to squamous cell carcinoma is warranted. This study highlights the progressively neoplastic nature of this lip lesion syndrome in sun bears which has consequences for captive and re-release management. Similarly, the detection of Ursid gammaherpesvirus 1 should be considered in pre-release risk analyses, at least until data is available on the prevalence of the virus in wild sun bears

Learning influences host choice in tsetse

A learning capacity for feeding is described in many insect species including vectors of diseases, but has never been reported in tsetse flies (Diptera, Glossinidae), the cyclic vectors of human (sleeping sickness) and animal trypanosomoses in Africa. Repeated feeding on the same host species by a disease vector is likely to increase the within-species disease-transmission risk, but to decrease it between species

Perception of health risks in Lao market vendors

Wet markets are a critical part of South-East Asian culture and economy. However, their role in circulation and transmission of both endemic and emerging disease is a source of concern in a region considered a hotspot of disease emergence. In the Lao People's Democratic Republic (Lao PDR, Laos), live and dead wild animals are frequently found in wet markets, despite legislation against the bushmeat trade. This is generally considered to increase the risk of disease transmission and emergence, although whether or not wildlife vendors themselves have indeed increased incidence of zoonotic disease has rarely been assessed. In preparation for a future longitudinal study of market vendors investigating vendors’ exposure to zoonotic pathogens, we conducted a pilot survey of Lao market vendors of wildlife meat, livestock meat and vegetables, to identify demographic characteristics and potential control groups within markets. We also investigated baseline risk perception for infectious diseases among market vendors and assessed the association between risk perception and risk mitigation behaviours. The surveys conducted with 177 vendors revealed similar age, sex, ethnic background and geographical origin between vendor types, but differences in professional background and work history for livestock meat vendors. The perception of disease risk was very low across all vendors, as was the reported use of personal protective equipment, and the two appeared unrelated. Personal risk discounting and assumptions about transmission routes may explain this lack of association. This information will help inform the development of future research, risk communication and risk mitigation policy, especially in the light of the COVID-19 pandemic

Zoonotic Pathogens in Wildlife Traded in Markets for Human Consumption, Laos

We tested animals from wildlife trade sites in Laos for the presence of zoonotic pathogens. Leptospira spp. were the most frequently detected infectious agents, found in 20.1% of animals. Rickettsia typhi and R. felis were also detected. These findings suggest a substantial risk for exposure through handling and consumption of wild animal meat

Experimental Comparative Study between Conventional and Green Parking Lots: Analysis of Subsurface Thermal Behavior under Warm and Dry Summer Conditions

Green infrastructure has a role to play in climate change adaptation strategies in cities. Alternative urban spaces should be designed considering new requirements in terms of urban microclimate and thermal comfort. Pervious pavements such as green parking lots can contribute to this goal through solar evaporative cooling. However, the cooling benefits of such systems remain under debate during dry and warm periods. The aim of this study was to compare experimentally the thermal behavior of different parking lot types (PLTs) with vegetated urban soil. Four parking lots were instrumented, with temperature probes buried at different depths. Underground temperatures were measured during summer 2019, and the hottest days of the period were analyzed. Results show that the less mineral used in the surface coating, the less it warms up. The temperature difference at the upper layer can reach 10 °C between mineral and non-mineral PLTs. PLTs can be grouped into three types: (i) high surface temperature during daytime and nighttime, important heat transfer toward the sublayers, and low time shift (asphalt system); (ii) high (resp. low) surface temperature during daytime (resp. nighttime), weak heat transfer toward the sublayers, and important time shift (paved stone system); and (iii) low surface temperature during daytime and nighttime, weak heat transfer toward the sublayers, and important time shift (vegetation and substrate system, wood chips system, vegetated urban soil). The results of this study underline that pervious pavements demonstrate thermal benefits under warm and dry summer conditions compared to conventional parking lot solutions. The results also indicate that the hygrothermal properties of urban materials are crucial for urban heat island mitigation