Competencias digitales para el profesorado universitario

“En el año 2013, la Comunidad Europea elabora el ``Marco Común Europeo de Competencias Digitales’’, o DigComp, que recoge las capacidades o habilidades que todo ciudadano debe tener en el ámbito digital para desarrollarse social y profesionalmente de una manera competente. Y, posteriormente, se desarrolla el ``Marco Común Europeo de Competencias Digitales Docentes’’, o DigCompEdu, centrado en el docente. Son documentos en continua evolución y que, basándose en ellos, permite a las instituciones elaborar criterios de evaluación y certificación de competencias. Las áreas en las que se agrupan las competencias digitales y que se dan a conocer en este libro son: información y alfabetización informacional, comunicación y colaboración, creación de contenidos digitales, seguridad y resolución de problemas

Tissue culture of ornamental cacti

Cacti species are plants that are well adapted to growing in arid and semiarid regions where the main problem is water availability. Cacti have developed a series of adaptations to cope with water scarcity, such as reduced leaf surface via morphological modifications including spines, cereous cuticles, extended root systems and stem tissue modifications to increase water storage, and crassulacean acid metabolism to reduce transpiration and water loss. Furthermore, seeds of these plants very often exhibit dormancy, a phenomenon that helps to prevent germination when the availability of water is reduced. In general, cactus species exhibit a low growth rate that makes their rapid propagation difficult. Cacti are much appreciated as ornamental plants due to their great variety and diversity of forms and their beautiful short-life flowers; however, due to difficulties in propagating them rapidly to meet market demand, they are very often over-collected in their natural habitats, which leads to numerous species being threatened, endangered or becoming extinct. Therefore, plant tissue culture techniques may facilitate their propagation over a shorter time period than conventional techniques used for commercial purposes; or may help to recover populations of endangered or threatened species for their re-introduction in the wild; or may also be of value to the preservation and conservation of the genetic resources of this important family. Herein we present the state-of-the-art of tissue culture techniques used for ornamental cacti and selected suggestions for solving a number of the problems faced by members of the Cactaceae family

Design Plan for an Evolution Study of Related Characteristics of a Population

The objective is to study the evolution of different characteristics of a population through time. These response variables may be related for each experimental unit, and in addition, the observations for each response may as well be correlated with time, producing a complex correlation structure. The number of responses that can be observed is usually limited for budget, resources, or time reasons, and thus the selection of the most informative time points when data must be taken is quite convenient. This will be performed by using the optimal design of experiments techniques. Some analytical results will be shown, and the results will be applied to obtain the most convenient points when tests about two variables related with the capability of the resolution of mathematical problems in primary school students should be performed

Design Plan for an Evolution Study of Related Characteristics of a Population

The objective is to study the evolution of different characteristics of a population through time. These response variables may be related for each experimental unit, and in addition, the observations for each response may as well be correlated with time, producing a complex correlation structure. The number of responses that can be observed is usually limited for budget, resources, or time reasons, and thus the selection of the most informative time points when data must be taken is quite convenient. This will be performed by using the optimal design of experiments techniques. Some analytical results will be shown, and the results will be applied to obtain the most convenient points when tests about two variables related with the capability of the resolution of mathematical problems in primary school students should be performed

Yeast One- and Two-Hybrid High-Throughput Screenings Using Arrayed Libraries

Since their original description more than 25 years ago, the yeast one- and two-hybrid systems (Y1H/Y2H) have been used by many laboratories to detect DNA?protein (Y1H) and protein?protein interactions (Y2H). These systems use yeast cells (Saccharomyces cerevisiae) as a eukaryotic ?test tube? and are amenable for most labs in the world. The development of highly efficient cloning methods has fostered the generation of large collections of open reading frames (ORFs) for several organisms that have been used for yeast screenings. Here, we describe a simple mating based method for high-throughput screenings of arrayed ORF libraries with DNA (Y1H) or protein (Y2H) baits not requiring robotics. One person can easily carry out this protocol in approximately 10 h of labor spread over 5 days. It can also be scaled down to test one-to-one (few) interactions, scaled up (i.e., robotization) and is compatible with several library formats (i.e., 96, 384-well microtiter plates)