Association of Calpastatin (CAST) Gene with Growth Traits and Carcass Characteristics in Bali Cattle

Calpastatin (CAST) gene is well known as an inhibitor of muscle protein degradation and relates to muscle growth and meat tenderness. The objective of this study was to determine the association of CAST gene with growth traits and carcass characteristics in Bali cattle. A number of data from 35 Bali bulls were collected from BPTU Bali Cattle to obtain growth traits, carcass characteristics, and blood samples. Polymorphism of CAST gene in Bali bulls was analyzed by using PCR-RFLP and DNA sequencing. The association of CAST gene with growth traits and carcass characteristics were analyzed by using General Linear Model (GLM). The result showed that there were two genotypes (GG and AG) of CAST gene with allele frequencies of 0.857 and 0.143, respectively, for G and A. Notably, mutation A to G occurred in 253 bp CAST fragment gene in Bali Cattle. Genotypes GG and AG of CAST gene significantly affected (P<0.05) the back-fat thickness and longissimus dorsi without a significant effect on the growth traits. It could be concluded that CAST gene had a potency as a marker gene for carcass quality in Bali cattle

The Growth and Distribution of Carcass Fat in Fattening Steer of Different Breeds

The growth and distribution patterns of carcass fat were investigated in three breeds of beef cattle entering fattening phase. The study involved 23 grass-fed steer Brahman, 24 Hereford and 22 Brahmanx Hereford crosses with a live weight range from 300-600 kg. An allometric Huxley model was used to study the growth and distribution patterns of fat tissue within wholesale cut. In most cases, Brahmans had significantly higher growth coefficients than Herefords and/or BrahmanxHereford crosses while Herefords and BrahmanxHereford crosses had similar growth coefficients in wholesale cuts. At log natural of 75 kg side muscle+bone weight (4.313 kg), Herefords had significantly a higher fat weight within wholesale cuts than Brahmans and BrahmanxHereford crosses. Comparison at log natural of 114 kg side muscle+bone weight (4.733 kg), whilst Brahmans and Herefords were similar in fat weight distribution, the cross-bred steer had lower fat weights than the other two breed groups in almost all wholesale cuts

Integrated Transport System Toward Sustainable Travel Behavior for Work Commuting Travel From Bekasi to Jakarta

Due to the inadequacy of public transport and high critical level of congestion in Jakarta Metropolitan Area, implementing sustainable transport for urban future transport improvement is necessary. Critical transport situation in Jakarta Metropolitan Area has pointed the importance of implementation integrated transport system to increase people accessibility. This study is conducted to identify strategic issues in integrated transport system at operational and policy levels toward sustainable mobility, transport equity, and door to door service. According to research aim, explanatory case study is used to build an understanding the current situation. The results indicate that integrated transport system is not fully implemented yet and it found a lot of missing links and barriers in integrated transport system attributes. Moreover, transportation planning at national to local levels is not synchronous which have impacted to the implementation of integrated transport

Muscle Growth and Distribution in Fattening Steer of Different Breeds

The breed patterns in growth and distribution of muscle were studied using three breeds of beef cattle entering fattening phase. This study involved 23 grass-fed steer Brahman, 24 Hereford and 22 Brahman x Hereford crosses with a live weight range of 300 to 600 kgs. An allometric Huxley model was used to study the growth and distribution patterns of muscle tissue within wholesale cut.  There were several cuts in which the muscle growth coefficients were significantly different among breeds. Comparisons of muscle weight distribution at log natural of 75 kg side muscle + bone weight (4.313 kg) generally showed significant between-breed differences. Herefords tended to have more muscles in their wholesale cuts than Brahmans and/or Brahman x Hereford crosses, except the muscles in Tenderloin, Rib Set and Chuck. Brahmans had heavier Topside and Silverside than Herefords and Brahman x Hereford crosses and the differences were maintained at log natural of 114 kg side muscle + bone weight (4.733 kg).  At this heavier weight, there was a general tendency for Brahmans to have more muscle in their wholesale cuts than the Brahman x Hereford steers, except the muscle in Loin and Neck + Sticking

Pertumbuhan Dan Distribusi Potongan Komersial Karkas Sapi Australian Commercial Cross Dan Brahman Cross Hasil Penggemukan

This research was conducted to study the growth and distribution of carcass components of beef carcas from Australian Commercial Cross and Brahman Cross cattle. The number of animals used was 165 heads with the body weight range of 350 - 400 kg taken from feedlot fattening. To study the growth and development of carcass component, the equation alometric Huxley was used. The result showed that breed had not significant effect on wholesale cuts. The geometry estimation on the specification of traditional markets and special market showed also the differences on topside (traditional market) and flank (special market)

Pengaruh Konformasi Butt Shape Terhadap Karakteristik Karkas Sapi Brahman Cross Pada Beberapa Klasifikasi Jenis Kelamin

Domestic demand on beef is increasing today. However the beef supply can not fulfil the demand so that importation of beef and feeder cattle is still required. Beef cattle feedloting is now growing in Indonesia. This research was done to study the growth and development of carcass components of beef carcas from Brahman Cross cattle. The number of animals used was 165 heads with the body weight range 350 - 400 kg taken from feedlot fattening. The experiment was set in completely randomized factorial design withh two factors, namely butt shape conformation (butt shape score D, C, B) and sex class (heifer, steer, cow). Parameter of carcass characteristic, i.e. carcass weight, carcass percentage, loin eye area, fat thickness of ribs 12th, fat percentage of kidney, pelvic and hearth, and fat thickness of rump P8.The result of this study showed that the increase of butt shape conformation score significantly increased loin eye area, especially in heifer and cow sex class

Association of Calpastatin (CAST) Gene with Growth Traits and Carcass Characteristics in Bali Cattle

Calpastatin (CAST) gene is well known as an inhibitor of muscle protein degradation and relates to muscle growth and meat tenderness. The objective of this study was to determine the association of CAST gene with growth traits and carcass characteristics in Bali cattle. A number of data from 35 Bali bulls were collected from BPTU Bali Cattle to obtain growth traits, carcass characteristics, and blood samples. Polymorphism of CAST gene in Bali bulls was analyzed by using PCR-RFLP and DNA sequencing. The association of CAST gene with growth traits and carcass characteristics were analyzed by using General Linear Model (GLM). The result showed that there were two genotypes (GG and AG) of CAST gene with allele frequencies of 0.857 and 0.143, respectively, for G and A. Notably, mutation A to G occurred in 253 bp CAST fragment gene in Bali Cattle. Genotypes GG and AG of CAST gene significantly affected (P<0.05) the back-fat thickness and longissimus dorsi without a significant effect on the growth traits. It could be concluded that CAST gene had a potency as a marker gene for carcass quality in Bali cattle.  Key words: Bali cattle, CAST gene, PCR-RFLP, polymorphis

Kualitas Daging Babi Dengan Pemberian Zeolit Dan Tepung Darah Sebagai Sumber Protein Dalam Ransum

Zeolite is a composite minerals from mining which can be used as a source of minerals in animal ration. It has high absorbability and cation exchange characteristics. This study was aimed to examine the influence of zeolit and blood meal in the pig ration on pork quality characterictics. The experiment used 27 crossbred pig comprising 19 gilts and 8 barrows, with initial weight of 25.29 + 2.67 kgs. They were set up in a completely randomized factorial design 3 x 3, where the first factor was zeolit levels (0, 3, and 6%) and the second factor was blood meal level (0, 5, and 10%). The results showed that there were no significant influence of zeolite, blood meal or their interaction on all meat quality characteristics including pH, water holding capacity, tenderness, color and intramuscular fat contents of pork, except the measurement at pH of six hours postmortem of the pork was significantly (P < 0.01) affected by blood meal level of the ration. Five percent (5%) blood meal in the ration gave the highest pH of pork compared to the other blood meal levels