STABILITY INDICATING HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF OFLOXACIN AND FLAVOXATE HYDROCHLORIDE

Objective: The objective of this study was to develop and validate a stability indicating reverse-phase HPLC method for simultaneous estimation of Ofloxacin and Flavoxate hydrochloride from their combination product.Methods: The proposed RP-HPLC method was developed using inertsil C18, 5 µm, 250 mm × 4.6 mm column. The mobile phase used was a mixture of methanol and water in the proportion of 50:50 (v/v) with apparent pH adjusted to 4.9, and UV detection at 274 nm using a PDA detector and Empower-2 software. The flow rate was 1.0 ml/min. Ofloxacin, Flavoxate hydrochloride and their combination drug product were exposed to thermal, photolytic, hydrolytic, and oxidative stress conditions, and the stressed samples were analysed by the proposed method.Results: With the optimized method, retention times of Ofloxacin and Flavoxate hydrochloride were found to be 4.3 and 2.98 respectively. Peak homogeneity data of Ofloxacin and Flavoxate hydrochloride peaks obtained using PDA detector, in the stressed sample chromatograms demonstrated the specificity of the method for their estimation in the presence of degradants. The described method was linear over a range of 10-60 µg/ml with regression coefficient of 0.9996 and 0.9998. The mean recoveries were 99.57% and 99.99% for Ofloxacin and Flavoxate hydrochloride, respectively.Conclusion: Stress testing, which covered acid, alkali, peroxide, photolytic and thermal degradation was performed to prove the specificity of the proposed method and degradation, was achieved. The developed method was validated according to ICH guidelines and was found to be simple, precise and accurate with the prescribed values.Â

Simulation of Drainage cleaning Robotics system using solid work Tinkercad software

In this paper we are designing simulation using tinkercad solid work software for simulation. In simulation we used pulse width modulation (PWM) to provide very smooth action and speed varieties .for PWM pulse we used ATmega2560 microcontroller. In this design we used robotic system which can reduce the complex mechanism for cleaning the manhole.it will give a clear understanding of the manipulator and its subsystem interactions

Automated Mechanism Generation Using Linear Scaling Relationships and Sensitivity Analyses Applied to Catalytic Partial Oxidation of Methane

Kinetic parameters for surface reactions can be predicted using a combination of DFT calculations, scaling relations, and machine learning algorithms; however, construction of microkinetic models still requires a knowledge of all the possible, or at least reasonable, reaction pathways. The recently developed Reaction Mechanism Generator (RMG) for heterogeneous catalysis, now included in RMG version 3.0, is built upon well-established, open-source software that can provide detailed reaction mechanisms from user-supplied initial conditions without making a priori assumptions. RMG is now able to estimate adsorbate thermochemistry and construct detailed microkinetic models on a range of hypothetical metal surfaces using linear scaling relationships. These relationships are a simple, computationally efficient way to estimate adsorption energies by scaling the energy of a calculated surface species on one metal to any other metal. By conducting simulations with sensitivity analyses, users can not only determine the rate limiting step on each surface by plotting a "volcano surface" for the degree of rate control of each reaction as a function of elemental binding energies, but also screen novel catalysts for desirable properties. We investigated the catalytic partial oxidation of methane to demonstrate the utility of this new tool and determined that an inlet gas C/O ratio of 0.8 on a catalyst with carbon and oxygen binding energies of -6.75 eV and -5.0 eV, respectively, yields the highest amount of synthesis gas. Sensitivity analyses show that while the dissociative adsorption of O2 has the highest degree of rate control, the interactions between individual reactions and reactor conditions are complex, which result in a dynamic rate-limiting step across differing metals.</div

Abstracts of Scientifica 2022

This book contains the abstracts of the papers presented at Scientifica 2022, Organized by the Sancheti Institute College of Physiotherapy, Pune, Maharashtra, India, held on 12–13 March 2022. This conference helps bring researchers together across the globe on one platform to help benefit the young researchers. There were six invited talks from different fields of Physiotherapy and seven panel discussions including over thirty speakers across the globe which made the conference interesting due to the diversity of topics covered during the conference. Conference Title:  Scientifica 2022Conference Date: 12–13 March 2022Conference Location: Sancheti Institute College of PhysiotherapyConference Organizer: Sancheti Institute College of Physiotherapy, Pune, Maharashtra, Indi