Frequency and Perceived Authenticity of Social Determinants of Health Discussion by Medical Trainees

Introduction Social determinants of health (SDOH)—the conditions in which people live, learn, and work—play a vital, but often neglected role in shaping a community’s health. SDOH influence risk factors for disease and access to healthcare, consequently promoting health inequities among different populations. Examining how providers discuss SDOH with patients can identify opportunities to better integrate social context into care. Methods We analyzed standardized patient (SP) encounters of rising UofL M3 trainees to 1) determine whether SDOH are integrated into healthcare conversations, and 2) investigate what constitutes an authentic conversation to identify how trainees can better express interest in a patient. SP encounters (n=41) were randomly sampled from 139 video recordings of new patient histories. Discussions concerning SDOH categories were coded for content, patient response, and the perceived authenticity of each interaction. Results The most frequently discussed SDOH was employment (80.49% of encounters) while financial security (0%), healthcare access (2.44%), and discrimination (2.44%) were among the least discussed. Trainees appeared more engaged and interested when they empathized with patients, provided reassurance, established personal connections, and displayed a logical/organized flow of thought. Discussion Clinical skills around SDOH could be improved if students were provided more practice incorporating patients’ answers about SDOH into the health management plan. SDOH discussions can be used to get to know the patient holistically and foster strong doctor-patient relationships, both of which are crucial communication/clinical skills assessed by licensing exams. Emphasis on SDOH in medical education can help students advance these skills.https://ir.library.louisville.edu/uars/1039/thumbnail.jp

A GATA-binding protein expressed predominantly in the pupal ovary of the silkworm, Bombyx mori

Preferential localization of Bkm (Banded krait minor-satellite) DNA sequences on Y/W chromosomes of higher eukaryotes, which remain highly condensed in somatic cells but undergo extensive decondensation in the germ cells during early stages of development, led to the postulation for the existence of a sex- and tissue-specific Bkm-binding protein (BBP). Accordingly, we purified and characterized a BmBBP expressed predominantly in pupal ovary of the silkworm (Bombyx mori). 2D-PAGE revealed BmBBP as moderately basic (pI 7.8-8, in the range expected for DNA-binding proteins) and Matrix Assisted Laser Desorption/Ionization Time of Flight exhibited a value of 37.5-kDa. BmBBP neither contains nor requires divalent metal ions for its DNA-binding activity, suggesting that it does not belong to the well-studied GATA-family of transcription factors. BmBBP is unusually strong in its DNA-binding characteristics to Bkm (GATA-repeats), which suggests its probable role in bringing about coordinated chromatin conformational changes to activate genes present in associated chromosomal domains. Fluorescence immuno-localization studies employing specific anti-BmBBP antibodies revealed its presence in the follicle cells and in the ooplasm, as well as the nucleus of different developmental stages of oocytes

Identification of the Genomic Insertion Site of the Thyroid Peroxidase Promoter–Cre Recombinase Transgene Using a Novel, Efficient, Next-Generation DNA Sequencing Method

Background: It can be useful to know the transgene insertion site in transgenic mice for a variety of reasons, but determining the insertion site generally is a time consuming, expensive, and laborious task. Methods: A simple method is presented to determine transgene insertion sites that combines the enrichment of a sequencing library by polymerase chain reaction (PCR) for sequences containing the transgene, followed by next-generation sequencing of the enriched library. This method was applied to determine the site of integration of the thyroid peroxidase promoter?Cre recombinase mouse transgene that is commonly used to create thyroid-specific gene deletions. Results: The insertion site was found to be between bp 12,372,316 and 12,372,324 on mouse chromosome 9, with the nearest characterized genes being Cntn5 and Jrkl, ?1.5 and 0.9?Mbp from the transgene, respectively. One advantage of knowing a transgene insertion site is that it facilitates distinguishing hemizygous from homozygous transgenic mice. Although this can be accomplished by real-time quantitative PCR, the expected Ct difference is only one cycle, which is challenging to assess accurately. Therefore, the transgene insertion site information was used to develop a 3-primer qualitative PCR assay that readily distinguishes wild type, hemizygous, and homozygous TPO-Cre mice based upon size differences of the wild type and transgenic allele PCR products. Conclusions: Identification of the genomic insertion site of the thyroid peroxidase promoter?Cre mouse transgene should facilitate the use of these mice in studies of thyroid biology.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/140267/1/thy.2015.0215.pd

Implementation of additive manufacturing in the healthcare supply chain for circular economy goals: Paradoxical tensions and solutions from an Industry 5.0 perspective

This study addresses the paradoxical tensions that arise during additive manufacturing (AM) implementation for circular economy goals in the healthcare sector. Using the lens of paradox theory, this study identifies four competing priorities that stakeholders may encounter while adopting AM. Focus group discussions among 12 industry experts from the healthcare supply chain were conducted to verify the paradoxes. Semi-structured interviews were then conducted with 10 industry experts to derive the solutions to manage these tensions from an Industry 5.0 perspective to achieve the full benefits of AM. This study expands paradox theory into the AM literature and provides a novel ‘both/and’ perspective (i.e. a pluralistic rather than a dualistic perspective) to look at emerging tensions encountered while implementing AM in the healthcare sector. This perspective will help decision-makers realise that these tensions can be managed over time to turn them into creative, rather than destructive, forces

Strain improvement for the production of antioxidant activity from Bacillus sp. by induced stress

In the present study, water samples were collected from different beaches in Chennai such as Marina beach, Elliott beach, VGP Golden beach and Kovalam beach to evaluate the best source for antioxidants. The bacteria were isolated on Starch Casein Agar media and screened for the antimicrobial activity. Among 11 isolates, 5 isolates showed antimicrobial potential which were further evaluated for the DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity. Among 5 isolates, one sample showed significant DPPH scavenging activity with half minimal inhibitory concentration of (IC50)344.754µg/mL. The maximum antioxidant production was observed at pH 7 and at temperature of 37ºC with an IC50 of 188.66µg/mL and 293.76µg/mL respectively. The potent antioxidant producing strain was subjected for mutagenesis. In physical mutagenesis, the organism exposed for UV light for 25 minutes showed maximum antioxidant production with an IC50 of 133.55µg/mL. This mutant strain was then subjected for chemical mutagenesis with the addition of different concentrations of Ethidium bromide such as 10µL, 20µL, 30µL, 40µL and 50µL. The mutant strain obtained with the addition of 20µL Ethidium bromide (EtBr) showed significant antioxidant activity with an IC50 of 325.4µg/mL. The sample was purified by solvent extraction method and was evaluated for antioxidant production. The analyte was subjected to HPLC (High Performance Liquid Chromatography) analysis to avince the presence of antioxidants. The ethyl acetate extract showed the total phenolic content of 0.892 mg GAE/g of dry extract. It also showed the total flavonoid content of 0.522mg RE/g of dry extract

Oxidative Stress and Regulation of Pink1 in Zebrafish (Danio rerio)

Peer reviewe

T-cell rich B-cell lymphoma of the breast: A rare case report with emphasis on the role of immunohistochemistry in the diagnosis

Primary breast lymphoma is one of the rare tumors that commonly presents as a lump with <0.5% incidence rate. It may be misdiagnosed as poorly differentiated carcinoma on routine fine-needle aspiration cytology. With appropriate treatment, this breast malignancy has a relatively better prognosis. Here, we report the case of a mammary T-cell rich B-cell non-Hodgkin lymphoma in a pre-menopausal woman diagnosed by biopsy and later confirmed by immunohistochemistry

Implementation of Additive Manufacturing in the Healthcare Supply Chain for Circular Economy Goals: Paradoxical Tensions and Solutions from an Industry 5.0 Perspective

Data Availability: The original dataset is available on request.This study addresses the paradoxical tensions that arise during additive manufacturing (AM) implementation for circular economy goals in the healthcare sector. Using the lens of paradox theory, this study identifies four competing priorities that stakeholders may encounter while adopting AM. Focus group discussions among 12 industry experts from the healthcare supply chain were conducted to verify the paradoxes. Semi-structured interviews were then conducted with 10 industry experts to derive the solutions to manage these tensions from an Industry 5.0 perspective to achieve the full benefits of AM. This study expands paradox theory into the AM literature and provides a novel ‘both/and’ perspective (i.e. a pluralistic rather than a dualistic perspective) to look at emerging tensions encountered while implementing AM in the healthcare sector. This perspective will help decision-makers realise that these tensions can be managed over time to turn them into creative, rather than destructive, forces