A newly developed tool for intra-tracheal temperature and humidity assessment in laryngectomized individuals: the Airway Climate Explorer (ACE)

The aim of this study is to develop a postlaryngectomy airway climate explorer (ACE) for assessment of intratracheal temperature and humidity and of influence of heat and moisture exchangers (HMEs). Engineering goals were within-device condensation prevention and fast response time characteristics. The ACE consists of a small diameter, heated air-sampling catheter connected to a heated sensor house, containing a humidity sensor. Air is sucked through the catheter by a controlled-flow pump. Validation was performed in a climate chamber using a calibrated reference sensor and in a two-flow system. Additionally, the analyser was tested in vivo. Over the clinically relevant range of humidity values (5–42 mg H2O/l air) the sensor output highly correlates with the reference sensor readings (R2 > 0.99). The 1–1/e response times are all <0.5 s. A first in vivo pilot measurement was successful. The newly developed, verified, fast-responding ACE is suitable for postlaryngectomy airway climate assessment

Modification of the L1-CAM carboxy-terminus in pancreatic adenocarcinoma cells

The neural cell adhesion molecule L1 has recently been shown to be expressed in pancreatic adenocarcinoma (PDAC) cells. In this report, we demonstrate that L1 is expressed by moderately- to poorly-differentiated PDAC cells in situ, and that L1 expression is a predictor of poor patient survival. In vitro, reduced reactivity of an anti-L1 carboxy-terminus-specific antibody was observed in the more poorly differentiated fast-growing (FG) variant of the COLO357 population, versus its well-differentiated slow-growing (SG) counterpart, even though they express equivalent total L1. The carboxy-terminus of L1 mediates binding to the MAP kinase-regulating protein RanBPM and mutation of T1247/S1248 within this region attenuates the expression of malignancy associated proteins and L1-induced tumorigenicity in mice. Therefore, we reasoned that the differential epitope exposure observed might be indicative of modifications responsible for regulating these events. However, epitope mapping demonstrated that the major determinant of binding was actually N1251; mutation of T1247 and S1248, alone or together, had little effect on C20 binding. Moreover, cluster assays using CD25 ectodomain/L1 cytoplasmic domain chimeras demonstrated the N1251-dependent, RanBPM-independent stimulation of erk phosphorylation in these cells. Reactivity of this antibody also reflects the differential exposure of extracellular epitopes in these COLO357 sublines, consistent with the previous demonstration of L1 ectodomain conformation modulation by intracellular modifications. These data further support a central role for L1 in PDAC, and define a specific role for carboxy-terminal residues including N1251 in the regulation of L1 activity in PDAC cells

Belediye Müzesi

Taha Toros Arşivi, Dosya No: 114-Müzelerİstanbul Kalkınma Ajansı (TR10/14/YEN/0033) İstanbul Development Agency (TR10/14/YEN/0033

Anatomical and Physiological Simulation for Respiratory Mechanics

Injuries in trauma affect anatomical structures, indirectly affecting physiological systems through mechanical behavior and physical proximity. This paper describes the theory and preliminary results from our approach to couple a 3D anatomical model of the chest with a physiological model of respiratory mechanics. In particular, we investigated behavior in quiet, normal breathing and an open sucking chest wound. We envision that our integrated simulation of respiratory anatomy and respiratory mechanics could assist students in visualizing and predicting relationships between structural-anatomical and functional-physiological changes in an interactive, 3D environment. 1 Introduction Trauma often involves direct anatomical injuries to the body that may cause indirect injuries from pathophysiological behavior of systems affected, particularly mechanical behavior. For example, a pneumothorax is a generic term for injuries that result in gas entering the intrapleural space, the space betwe..

Retinopexia pneumática e fotocooagulação a laser para tratamento de descolamento secundário à fosseta de disco óptico: relato de caso Pneumatic retinopexy and laser photocoagulation for treatment of optic disc pit detachment: case report

Os autores relatam o caso de um paciente com fosseta congênita de disco óptico associado a descolamento seroso macular, tratado com sucesso por meio de fotocoagulação com laser de argônio na borda temporal da fosseta, seguido de injeção intravítrea de 0,3 ml de C3F8 (100%). O paciente foi seguido por 12 meses. A resolução do descolamento foi acompanhada de melhora da acuidade visual, bem como o retorno da fóvea a sua configuração normal, comprovada através da tomografia de coerência óptica.<br>The authors report a case of congenital optic disc pit with serous macular detachment successfully treated, with argon laser photocoagulation in the temporal border of the pit, followed by intravitreous injection of C3F8 (100%) 0.3 ml. Patient was followed up for 12 months. Retinal reattachment was associated with improvement in visual acuity, as well as return of the fovea to its normal configuration shown by optical coherence tomography