Extranuclear structural components that mediate dynamic chromosome movements in yeast meiosis

Telomere-led rapid chromosome movements or rapid prophase movements direct fundamental meiotic processes required for successful haploidization of the genome. Critical components of the machinery that generates rapid prophase movements are unknown, and the mechanism underlying rapid prophase movements remains poorly understood. We identified S. cerevisiae Mps2 as the outer nuclear membrane protein that connects the LINC complex with the cytoskeleton. We also demonstrate that the motor Myo2 works together with Mps2 to couple the telomeres to the actin cytoskeleton. Further, we show that Csm4 interacts with Mps2 and is required for perinuclear localization of Myo2, implicating Csm4 as a regulator of the Mps2-Myo2 interaction. We propose a model in which the newly identified functions of Mps2 and Myo2 cooperate with Csm4 to drive chromosome movements in meiotic prophase by coupling telomeres to the actin cytoskeleton.Fil: Lee, Chih Ying. Oklahoma Medical Research Foundation; Estados UnidosFil: Bisig, Carlos Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Conrad, Michael M.. Oklahoma Medical Research Foundation; Estados UnidosFil: Ditamo, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Previato de Almeida, Luciana. Oklahoma Medical Research Foundation; Estados UnidosFil: Dresser, Michael E.. Oklahoma Medical Research Foundation; Estados UnidosFil: Pezza, Roberto J.. Oklahoma Medical Research Foundation; Estados Unido

Cardiac injury and mortality in patients with Coronavirus disease 2019 (COVID-19): insights from a mediation analysis

Patients at greatest risk of severe clinical conditions from coronavirus disease 2019 (COVID-19) and death are elderly and comorbid patients. Increased levels of cardiac troponins identify patients with poor outcome. The present study aimed to describe the clinical characteristics and outcomes of a cohort of Italian inpatients, admitted to a medical COVID-19 Unit, and to investigate the relative role of cardiac injury on in-hospital mortality

Telomere-led meiotic chromosome movements: recent update in structure and function

In S. cerevisiae prophase meiotic chromosomes move by forces generated in the cytoplasm and transduced to the telomere via a protein complex located in the nuclear membrane. We know that chromosome movements require actin cytoskeleton [13,31] and the proteins Ndj1, Mps3, and Csm4. Until recently, the identity of the protein connecting Ndj1-Mps3 with the cytoskeleton components was missing. It was also not known the identity of a cytoplasmic motor responsible for interacting with the actin cytoskeleton and a protein at the outer nuclear envelope. Our recent work [36] identified Mps2 as the protein connecting Ndj1-Mps3 with cytoskeleton components; Myo2 as the cytoplasmic motor that interacts with Mps2; and Cms4 as a regulator of Mps2 and Myo2 interaction and activities (Figure 1). Below we present a model for how Mps2, Csm4, and Myo2 promote chromosome movements by providing the primary connections joining telomeres to the actin cytoskeleton through the LINC complex.Fil: Lee, C. Y.. Oklahoma Medical Research Foundation; Estados UnidosFil: Bisig, Carlos Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; ArgentinaFil: Conrad, M. N.. Oklahoma Medical Research Foundation; Estados UnidosFil: Ditamo, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; ArgentinaFil: Almeida, L. Previato de. Oklahoma Medical Research Foundation; Estados UnidosFil: Dresser, M.E.. Oklahoma Medical Research Foundation; Estados UnidosFil: Pezza, Roberto. Oklahoma Medical Research Foundation; Estados Unido

Il progetto Interreg Italia-Slovenia HARMO-DATA: un geoportale per la condivisione di dati territoriali transfrontalieri armonizzati

Il progetto Interreg HARMO-DATA, finanziato nell’ambito del Programma Interreg V-A Italia-Slovenia 2014-2020, è stato avviato ufficialmente a Settembre 2017 e si è concluso il 30 giugno 2019. Il Partenariato era composto da: Geodetski In titut Slovenije, Ljubljana, Slovenia (coordinamento), Geodetska Uprava Republike Slovenije, Igea S.r.l. Ljubljana, Slovenia, Insiel S.p.a. Trieste, GeoSNav Lab - Dipartimento di Ingegneria e Architettura, Università degli Studi di Trieste - Terre S.r.l. Mestre. Hanno partecipato inoltre in qualità di partner associati: Regione Veneto, Regione Friuli Venezia Giulia e Ministero sloveno per l’ambiente ed il territorio. Obiettivo generale del Progetto è stato quello di rafforzare la capacità di cooperazione istituzionale transfrontaliera tra le autorità pubbliche e i responsabili della pianificazione territoriale, promuovendo la creazione di soluzioni condivise per il coordinamento e un’efficace gestione del territorio

PROJEKT HARMO-DATA: USKLADITEV PODATKOV ZA \u10cEZMEJNO UPRAVLJANJE PROSTORA (PROJECT HARMO-DATA: DATA HARMONIZATION FOR CROSS-BORDER SPATIAL MANAGEMENT)

HARMO-DATA je projekt, financiran v okviru programa sodelovanja INTERREG V-A Italija-Slovenija 2014\u20132020, ki ga sofinancira Evropski sklad za regionalni razvoj. Uradno se je za\u10del izvajati septembra 2017, projektne dejavnosti so bile dokon\u10dane 30. 6. 2019. V njem je sodelovalo \u161est partnerjev iz Slovenije in Italije, in sicer Geodetski in\u161titut Slovenije (koordiniranje), Geodetska uprava Republike Slovenije, Igea, d. o. o., Insiel, S. p. A. (Trst), GeoSNav Lab \u2013 Oddelek za in\u17eenirstvo in arhitekturo, Univerza v Trstu, in Terre, s. r. l. (Mestre). Pri projektu so sodelovali tudi pridru\u17eeni partnerji de\u17eel Bene\u10dija in Furlanija - Julijska krajina ter ministrstvo za okolje in prostor Republike Slovenije. Glavni cilj projekta je iskanje skupnih re\u161itev za izbolj\u161anje upravljanja prostora in uporabe \u10dezmejnih prostorskih podatkov, uskladitev obstoje\u10dih prostorskih podatkov, razvoj skupne \u10dezmejne prostorske platforme, priprava skupnega protokola za dostop do harmoniziranih podatkov ter njihovo vzdr\u17eevanje. V projekt so bile vklju\u10dene interesne skupine in uporabniki prostorskih podatkov: oblikovalci politik, nacionalne, regionalne in lokalne organizacije, civilna za\u161\u10dita, podjetja, univerze in raziskovalna sredi\u161\u10da. U\u10dinkovita uporaba \u10dezmejnih prostorskih podatkov je mogo\u10da le, \u10de so harmonizirani. Model harmonizacije podatkov, ki smo ga izbrali v projektu, je INSPIRE in je v \u10dlanku predstavljen skupaj z drugimi rezultati projekta (A\u17eman et al., 2018)

Shugoshin protects centromere pairing and promotes segregation of nonexchange partner chromosomes in meiosis

© 2019 the Author(s).Faithful chromosome segregation during meiosis I depends upon the formation of connections between homologous chromosomes. Crossovers between homologs connect the partners, allowing them to attach to the meiotic spindle as a unit, such that they migrate away from one another at anaphase I. Homologous partners also become connected by pairing of their centromeres in meiotic prophase. This centromere pairing can promote proper segregation at anaphase I of partners that have failed to become joined by a crossover. Centromere pairing is mediated by synaptonemal complex (SC) proteins that persist at the centromere when the SC disassembles. Here, using mouse spermatocyte and yeast model systems, we tested the role of shugoshin in promoting meiotic centromere pairing by protecting centromeric synaptonemal components from disassembly. The results show that shugoshin protects the centromeric SC in meiotic prophase and, in anaphase, promotes the proper segregation of partner chromosomes that are not linked by a crossover.The authors thank the members of the Program in Cell Cycle and Cancer Biology for their constructive comments during this project. A.M.P. is funded by Award BFU2017-89408-R from the Spanish Ministry of Economy and Competitiveness and his program is supported by the Programa de Apoyo a Planes Estratégicos de Investigación de Estructuras de Investigación de Excelencia, which is cofunded by the Castilla–León autonomous government and the European Regional Development Fund (Award CLC–2017–01). R.J.P. was supported by Centers of Biomedical Research Excellence Grant GM103636 and March of Dimes Grant FY14-256. D.S.D. was supported by NIH Grant R01 GM087377