487 research outputs found
Stereopsis in sports: Visual skills and visuomotor integration models in professional and non-professional athletes
Visual skills in sport are considered relevant variables of athletic performance. However, data on the specific contribution of stereopsis—as the ability to perceive depth—in sport performance are still scarce and scattered in the literature. The aim of this review is therefore to take stock of the effects of stereopsis on the athletic performance, also looking at the training tools to improve visual abilities and potential differences in the visuomotor integration processes of professional and non-professional athletes. Dynamic stereopsis is mainly involved in catching or interceptive actions of ball sports, whereas strategic sports use different visual skills (peripheral and spatial vision) due to the sport-specific requirements. As expected, professional athletes show better visual skills as compared to non-professionals. However, both non-professional and professional athletes should train their visual skills by using sensory stations and light boards systems. Non-professional athletes use the visual inputs as the main method for programming motor gestures. In contrast, professional athletes integrate visual information with sport expertise, thus, they encode the match (or the athletic performance) through a more complex visuomotor integration system. Although studies on visual skills and stereopsis in sports still appear to be in their early stages, they show a large potential for both scientific knowledge and technical development
Thyroid-specific transcription factors control Hex promoter activity
The homeobox-containing gene Hex is expressed in several cell types, including thyroid follicular cells, in which it regulates the transcription of tissue-specific genes. In this study the regulation of Hex promoter activity was investigated. Using co-transfection experiments, we demonstrated that the transcriptional activity of the Hex gene promoter in rat thyroid FRTL-5 cells is ∼10-fold greater than that observed in HeLa and NIH 3T3 cell lines (which do not normally express the Hex gene). To identify the molecular mechanisms underlying these differences, we evaluated the effect of the thyroid-specific transcription factor TTF-1 on the Hex promoter activity. TTF-1 produced 3-4-fold increases in the Hex promoter activity. Gel-retardation assays and mutagenesis experiments revealed the presence of functionally relevant TTF-1 binding sites in the Hex promoter region. These in vitro data may also have functional relevance in vivo, since a positive correlation between TTF-1 and Hex mRNAs was demonstrated in human thyroid tissues by means of RT-PCR analysis. The TTF-1 effect, however, is not sufficient to explain the difference in Hex promoter activity between FRTL-5 and cells that do not express the Hex gene. For this reason, we tested whether Hex protein is able to activate the Hex promoter. Indeed, co-transfection experiments indicate that Hex protein is able to increase the activity of its own promoter in HeLa cells ∼4-fold. TTF-1 and Hex effects are additive: when transfected together in HeLa cells, the Hex promoter activity is increased 6-7-fold. Thus, the contemporary presence of both TTF-1 and Hex could be sufficient to explain the higher transcriptional activity of the Hex promoter in thyroid cells with respect to cell lines that do not express the Hex gene. These findings demonstrate the existence of direct cross-regulation between thyroid-specific transcription factors
Buffering Adaptive Immunity by Hydrogen Sulfide
Abstract: T cell-mediated adaptive immunity is designed to respond to non-self antigens and
pathogens through the activation and proliferation of various T cell populations. T helper 1 (Th1),
Th2, Th17 and Treg cells finely orchestrate cellular responses through a plethora of paracrine and
autocrine stimuli that include cytokines, autacoids, and hormones. Hydrogen sulfide (H2S) is one
of these mediators able to induce/inhibit immunological responses, playing a role in inflammatory
and autoimmune diseases, neurological disorders, asthma, acute pancreatitis, and sepsis. Both endogenous
and exogenous H2S modulate numerous important cell signaling pathways. In monocytes,
polymorphonuclear, and T cells H2S impacts on activation, survival, proliferation, polarization,
adhesion pathways, and modulates cytokine production and sensitivity to chemokines. Here, we
offer a comprehensive review on the role of H2S as a natural buffer able to maintain over time a
functional balance between Th1, Th2, Th17 and Treg immunological responses
Different waters for different performances: Can we imagine sport-related natural mineral spring waters?
Preserving the hydration status means to balance daily fluids and salt losses with gains, where the losses depend on several physiological and environmental factors. Especially for athletes, these losses could be relevant and negatively influence the performance: therefore, their hydro-saline status must be preserved with personalized pre-and rehydration plans all along the performance period. Scientific literature in this field is mainly dedicated to artificial sport drinks. Different territories in most world areas are rich in drinking natural mineral spring waters with saline compositions that reflect their geological origin and that are used for human health (often under medical prescription). However, scarce scientific attention has been dedicated to the use of these waters for athletes. We therefore reviewed the existing literature from the innovative viewpoint of matching spring water mineral compositions with different athletic performances and their hydro-saline requirements
Kinetic Inductance Detectors for the OLIMPO experiment: design and pre-flight characterization
We designed, fabricated, and characterized four arrays of horn--coupled,
lumped element kinetic inductance detectors (LEKIDs), optimized to work in the
spectral bands of the balloon-borne OLIMPO experiment. OLIMPO is a 2.6 m
aperture telescope, aimed at spectroscopic measurements of the
Sunyaev-Zel'dovich (SZ) effect. OLIMPO will also validate the LEKID technology
in a representative space environment. The corrected focal plane is filled with
diffraction limited horn-coupled KID arrays, with 19, 37, 23, 41 active pixels
respectively at 150, 250, 350, and 460GHz. Here we report on the full
electrical and optical characterization performed on these detector arrays
before the flight. In a dark laboratory cryostat, we measured the resonator
electrical parameters, such as the quality factors and the electrical
responsivities, at a base temperature of 300mK. The measured average
resonator s are 1.7, 7.0, 1.0, and
1.0 for the 150, 250, 350, and 460GHz arrays, respectively.
The average electrical phase responsivities on resonance are 1.4rad/pW,
1.5rad/pW, 2.1rad/pW, and 2.1rad/pW; the electrical noise
equivalent powers are 45, 160,
80, and 140, at 12 Hz. In the OLIMPO
cryostat, we measured the optical properties, such as the noise equivalent
temperatures (NET) and the spectral responses. The measured NETs are
, , ,
and , at 12 Hz; under 78, 88, 92, and 90 mK
Rayleigh-Jeans blackbody load changes respectively for the 150, 250, 350, and
460 GHz arrays. The spectral responses were characterized with the OLIMPO
differential Fourier transform spectrometer (DFTS) up to THz frequencies, with
a resolution of 1.8 GHz.Comment: Published on JCA
TR-644 a novel potent tubulin binding agent induces impairment of endothelial cells function and inhibits angiogenesis.
TR-644 is a novel combretastatin A-4 (CA-4) analogue endowed with potent microtubule depolymerizing activity superior to that of the lead compound and it also has high affinity to colchicines binding site of tubulin. We tested TR-644 anti-angiogenic effects in human umbilical endothelial cells (HUVEC). It showed no significant effects on the growth of HUVEC cells at concentrations below 1,000 nM, but at much lower concentrations (10-100 nM) it induced inhibition of capillary tube formation, inhibition of endothelial cell migration and affected endothelial cell morphology as demonstrated by the disruption of the microtubule network. TR-644 also increased permeability of HUVEC cells in a time dependent manner. The molecular mechanism for the anti-vascular activity of TR-644 was investigated in detail. TR-644 caused G2/M arrest in endothelial cells and this effect correlated with downregulation of the expression of Cdc25C and Cdc2Tyr15. Moreover TR-644 inhibited VEGF-induced phosphorylation of VE-cadherin but did not prevent the VEGF-induced phosphorylation of FAK. In chick chorioallantoic membrane in vivo assay, TR-644 (0.1-1.0 pmol/egg) efficiently counteracted the strong angiogenic response induced by FGF. Also CA-4, used as reference compound, caused an antagonistic effect, but in contrast, it induced per se, a remarkable angiogenic response probably due to an inflammatory reaction in the site of treatment. In a mice allogenic tumor model, immunohistochemical staining of tumors with anti-CD31 antibody showed that TR-644 significantly reduced the number of vessel, after 24 h from the administration of a single dose (30 mg/Kg)
Physical activity and redox balance in the elderly: Signal transduction mechanisms
Reactive Oxygen Species (ROS) are molecules naturally produced by cells. If their levels are too high, the cellular antioxidant machinery intervenes to bring back their quantity to physiological conditions. Since aging often induces malfunctioning in this machinery, ROS are considered an effective cause of age-associated diseases. Exercise stimulates ROS production on one side, and the antioxidant systems on the other side. The effects of exercise on oxidative stress markers have been shown in blood, vascular tissue, brain, cardiac and skeletal muscle, both in young and aged people. However, the intensity and volume of exercise and the individual subject characteristics are important to envisage future strategies to adequately personalize the balance of the oxidant/antioxidant environment. Here, we reviewed the literature that deals with the effects of physical activity on redox balance in young and aged people, with insights into the molecular mechanisms involved. Although many molecular pathways are involved, we are still far from a comprehensive view of the mechanisms that stand behind the effects of physical activity during aging. Although we believe that future precision medicine will be able to transform exercise administration from wellness to targeted prevention, as yet we admit that the topic is still in its infancy
Fibroblast growth factor 2-antagonist activity of a long-pentraxin 3-derived antiangiogenic pentapeptide.
Fibroblast growth factor-2 (FGF2) plays a major role in angiogenesis. The pattern recognition receptor long-pentraxin 3 (PTX3) inhibits the angiogenic activity of FGF2. To identify novel FGF2-antagonistic peptide(s), four acetylated (Ac) synthetic peptides overlapping the FGF2-binding region PTX3-(97-110) were assessed for their FGF2-binding capacity. Among them, the shortest pentapeptide Ac-ARPCA-NH(2) (PTX3-[100-104]) inhibits the interaction of FGF2 with PTX3 immobilized to a BIAcore sensorchip and suppresses FGF2-dependent proliferation in endothelial cells, without affecting the activity of unrelated mitogens. Also, Ac-ARPCA-NH(2) inhibits angiogenesis triggered by FGF2 or by tumorigenic FGF2-overexpressing murine endothelial cells in chick and zebrafish embryos, respectively. Accordingly, the peptide hampers the binding of FGF2 to Chinese Hamster ovary cells overexpressing the tyrosine-kinase FGF receptor-1 (FGFR1) and to recombinant FGFR1 immobilized to a BIAcore sensorchip without affecting heparin interaction. In all the assays the mutated Ac-ARPSA-NH(2) peptide was ineffective. In keeping with the observation that hydrophobic interactions dominate the interface between FGF2 and the FGF-binding domain of the Ig-like loop D2 of FGFR1, amino acid substitutions in Ac-ARPCA-NH(2) and saturation transfer difference-nuclear magnetic resonance analysis of its mode of interaction with FGF2 implicate the hydrophobic methyl groups of the pentapeptide in FGF2 binding. These results will provide the basis for the design of novel PTX3-derived anti-angiogenic FGF2 antagonists
The long duration cryogenic system of the OLIMPO balloon--borne experiment: design and in--flight performance
We describe the design and in--flight performance of the cryostat and the
self-contained He refrigerator for the OLIMPO balloon--borne experiment,
a spectrophotometer to measure the Sunyaev-Zel'dovich effect in clusters of
galaxies.
The He refrigerator provides the 0.3 K operation temperature for the
four arrays of kinetic inductance detectors working in 4 bands centered at 150,
250, 350 and 460 GHz. The cryostat provides the 1.65 K base temperature for the
He refrigerator, and cools down the reimaging optics and the filters
chain at about 2 K.
The integrated system was designed for a hold time of about 15 days, to
achieve the sensitivity required by the planned OLIMPO observations, and
successfully operated during the first long-duration stratospheric flight of
OLIMPO in July 2018.
The cryostat features two tanks, one for liquid nitrogen and the other one
for liquid helium. The long hold time has been achieved by means of custom
stiff G10 fiberglass tubes support, which ensures low thermal conductivity and
remarkable structural stiffness; multi--layer superinsulation, and a vapour
cooled shield, all reducing the heat load on the liquid helium tank.
The system was tested in the lab, with more than 15 days of unmanned
operations, and then in the long duration balloon flight in the stratosphere.
In both cases, the detector temperature was below 300 mK, with thermal
stability better than 0.5 mK.
The system also operated successfully in the long duration stratospheric
balloon flight
Clinical outcomes and temporal trends of immunological and non-immunological rare diseases in adult kidney transplant
- …