Effect of Phaseolus vulgaris on E. coli induced peritonitis and bacteraemia in mice

Infectious or non-infectious peritonitis leads to systemic inflammation due to violation of the peritoneum which is often fatal. Evidences suggest that common bean (Phaseolus vulgaris L.) is a source of nutrients and contains phenolic compounds having antioxidant activity and its consumption has been linked with improved health benefits. The aim of the present investigation was evaluate the in vitro antibacterial, antioxidant activity and protective potential of the methanolic extract of P. vulgaris in E. coli induced model of peritonitis in albino wistar rats. Rats were pre-treated with 200 mg/kg and 400 mg/kg/bwt dose for 3 days and fourth day with E. coli (1×108 CFU/ml) strain and consecutively 3 days treatment. Mortality was monitored for 14 days. After the death of rats or completion of the experiment rats were sacrifice and kidney were used for our protocol. Colonies were count and statically analysis was done. Results showed dose dependent antibacterial activity. Thus the methanolic extract of P. vulgaris exhibited significant protection against E. coli induced peritonitis in normal rats. It significantly reduced the viable cells of E. coli when inoculated in rats. Activity is attributed to flavonoids and phenolic compounds. The present study thus suggests that methanolic extract of P. vulgaris significantly reverses peritoneal infection by E. coli in rats. It can be suggest that this medicinal formulation will be used as herbal medicine with no side effects. The high content of phenolic compounds, antioxidant activity and antibacterial activity of P. vulgaris indicate that they may impart health benefits when consumed and should be regarded as a valuable source of antioxidants. Thus, consumption of P. vulgaris seed along with coats might be recommended to gaining better nutritive benefits. Keywords:  P. vulgaris, Peritonitis, Antioxidant, Antibacterial, E. col

Gastrointestinal stromal tumour in Meckel's diverticulum

<p>Abstract</p> <p>Background</p> <p>Meckel's Diverticulum is the most commonly encountered congenital anomaly of the small intestine, occurring in approximately 2% of the population. Occasionally Meckel's diverticulum harbors neoplasms.</p> <p>Case presentation</p> <p>A 65 year old gentleman, presented with a pelvic mass. On exploratory laparotomy, it turned out to be gastrointestinal stromal tumour (GIST) arising from Meckel's diverticulum. Short history and review of literature are discussed.</p> <p>Conclusion</p> <p>Neoplasms occurring from Meckel's diverticulum, even though rare, should be considered as differential diagnosis of pelvic masses arising from bowel, wherever imaging modalities fail to give a definitive diagnosis.</p

Transcriptomic and metabolomic shifts in rice roots in response to Cr (VI) stress

<p>Abstract</p> <p>Background</p> <p>Widespread use of chromium (Cr) contaminated fields due to careless and inappropriate management practices of effluent discharge, mostly from industries related to metallurgy, electroplating, production of paints and pigments, tanning, and wood preservation elevates its concentration in surface soil and eventually into rice plants and grains. In spite of many previous studies having been conducted on the effects of chromium stress, the precise molecular mechanisms related to both the effects of chromium phytotoxicity, the defense reactions of plants against chromium exposure as well as translocation and accumulation in rice remain poorly understood.</p> <p>Results</p> <p>Detailed analysis of genome-wide transcriptome profiling in rice root is reported here, following Cr-plant interaction. Such studies are important for the identification of genes responsible for tolerance, accumulation and defense response in plants with respect to Cr stress. Rice root metabolome analysis was also carried out to relate differential transcriptome data to biological processes affected by Cr (VI) stress in rice. To check whether the Cr-specific motifs were indeed significantly over represented in the promoter regions of Cr-responsive genes, occurrence of these motifs in whole genome sequence was carried out. In the background of whole genome, the lift value for these 14 and 13 motifs was significantly high in the test dataset. Though no functional role has been assigned to any of the motifs, but all of these are present as promoter motifs in the Database of orthologus promoters.</p> <p>Conclusion</p> <p>These findings clearly suggest that a complex network of regulatory pathways modulates Cr-response of rice. The integrated matrix of both transcriptome and metabolome data after suitable normalization and initial calculations provided us a visual picture of the correlations between components. Predominance of different motifs in the subsets of genes suggests the involvement of motif-specific transcription modulating proteins in Cr stress response of rice.</p

Study and Analysis of MVDR DOA Estimation Algorithm xyz

-The smart antenna (SA} can adjust the direction pattern adaptively and reduce the interference signals using some adaptive interference mulling algorithms, and thus enhances the performance of mobile telecommunication system, especially enlarges the user capacity of system. The smart antenna system is mainly applied to the base station (BS) now, but next step, the smart antenna technology will be applied to mobile station (MS) along with hardware and software in MS becoming more powerful. The smart antenna system will be applied in both BS and MS in next generation mobile telecommunication system. However, performance of smart antenna system greatly depends on efficiency of digital signal processing algorithms. The algorithm uses the Direction of Arrival (DOA) algorithms to estimate the number of incidents plane waves on the antenna array and their angle of incidence. In this paper the performance of Direction-of-Arrival (DOA) algorithm MVDR is investigated. The simulation results show that, the advantages in performance of one algorithm over another vary with the conditions and is significantly influenced by both of the environments well as the system. Thus, careful consideration is imperative to the conditions and system parameters specific to the planned deployment. The algorithms have been simulated in MATLAB 7.4 version

Micropropagation of Wrightia tomentosa : Effect of gelling agents, carbon source and vessel type

115-120The role of gelling agents, carbon source, type of culture vessel and liquid culture system during rooting phase in Wrightia tomentosa was investigated in an effort to reduce the cost of micropropagation. Studies have revealed significant improvement in shoot multiplication on a medium containing crude agar and sugarcubes. Promotory role of vented vessel in culture growth and liquid culture system during rooting phase was established. The results showed strong potential for reduction in cost of plantlets in vitro

Study on types of anaemia and foetomaternal outcome in antenatal patients

Anemia is the most common haematological disorder in pregnancy. Anemia increases maternal morbidity and mortality. It is a condition that can be diagnosed and treated during antenatal period, preventing the serious complications of anemia during pregnancy and labour. Aims: This study investigated the prevalence of types of anemia during pregnancy and fetomaternal outcomes among pregnant women. Study Design: This was a cross-sectional study that enrolled 1100 pregnant women who were in the third trimester of pregnancy with haemoglobin level <11.0 gm/dl between March 2019 and August 2020. Methods and Material: Participants were selected by consecutive sampling and baseline data were collected by using a predesigned and pretested structured questionnaire. Data Analysis: Data were entered and analysed by using SPSS version 20. Results: The prevalence of anemia in third-trimester pregnancy in this study was 91.05%. Iron deficiency anemia was most common (69.18%) among pregnant women followed by megaloblastic anemia (2.5%). Most of pregnant women (45.90%) were mildly anemic. Mean ± SD of haematological parameters among the anemic pregnant women during third trimester of pregnancy were haemoglobin (8.08±2.24 gm/dl), haematocrit (28.92±7.78%), mean corpuscular volume (MCV) (93.02±11.32fl), mean corpuscular haemoglobin (MCH) (26.03±2.90 pg), mean corpuscular haemoglobin concentration(MCHC) (27.99 ± 2.01 gm/dl), RBC count (3.05 ± 0.67million/mm3). Most common maternal complications due to anemia in pregnancy was preterm labour (30%). Fetal outcome in the form of an alive term, most commonly seen in mild anemia (34.7%), fetal complications like Preterm alive (11.3%), preterm intrauterine fetal death (IUFD) (2.7%) most commonly associated with severe anemia. Conclusion: The present study concludes that the prevalence of anemia among pregnant women in third trimester of pregnancy was 91.05% which is a serious public health problem. Proper counselling to the patients and their family members regarding cause of anemia, effect of anemia and complications of anemia. So, that such preventable condition can be prevented

Arsenomics: Omics of Arsenic Metabolism in Plants

AbstractArsenic (As) contamination of drinking water and groundwater used for irrigation can lead to contamination of the food chain and poses serious health risk to people worldwide. To reduce As intake through the consumption of contaminated food, identification of the mechanisms for As accumulation and detoxification in plant is a prerequisite to develop efficient phytoremediation methods and safer crops with reduced As levels. Transcriptome, proteome and metabolome analysis of any organism reflects the total biological activities at any given time which are responsible for the adaptation of the organism to the surrounding environmental conditions. As these approaches are very important in analyzing plant As transport and accumulation, we termed Arsenomics as approach which deals transcriptome, proteome and metabolome alterations during As exposure. Although, various studies have been performed to understand modulation in transcriptome in response to As, many important questions need to be addressed regarding the translated proteins of plants at proteomic and metabolomic level, resulting in various ecophysiological responses. In this review, the comprehensive knowledge generated in this area has been compiled and analyzed. There is a need to strengthen Arsenomics which will lead to develop of tools to develop As-free plants for safe consumption