Self-assembled high molecular weight inulin nanoparticles: Enzymatic synthesis, physicochemical and biological properties

Inulin has interesting physicochemical and functional properties, and therefore a wide range of applications in the food and medical industries. It has gained great traction due to its ability to form nanoparticles and its possible application as nanovehicle for drug delivery. In this work, we demonstrated that the enzymatically-synthesized high molecular weight (HMW) inulin forms stable spherical nanoparticles with an average diameter of 112 ± 5 nm. The self-assemblage of HMW inulin nanoparticles is carried out during enzymatic synthesis of the polymer, and become detectable after a certain critical aggregation concentration (CAC) is reached. Both, the CAC and nanoparticle size are influenced by the reaction temperature. These nanoparticles are not toxic for peripheral blood mononuclear cells, at concentrations below 200 μg/mL; no significant prebiotic potential was detected in cultures of 13 probiotic strains. This work contributes to a better understanding of the formation of HMW inulin nanoparticles and their biological properties

Wplyw terminu zbioru na zawartosc aminokwasow w bulwach ziemniakow

Four very early varieties of edible potatoes Aster, Drop, Malwa and Orlik cutivated in 1992-1993 on the experimental fields in Southern Poland were examined for the contents of solids, total nitrogen, protein nitrogen, and amino acids after 60, 70, 80, and 120 days of vegetation. The potatoes were characterized by the average content of total nitrogen (1.38% dry weight) and high content of protein nitrogen (1.07% dry weight). Statistically significant correlations were found between the contents of total nitrogen and total amino acids (r = 0.83), and the contents of protein nitrogen and total essential amino acids (r = 0.74) or endogenous amino acids (r = 0.80).W bulwach czterech bardzo wczesnych odmian jadalnych ziemniaków (Aster, Drop, Malwa, Orlik), uprawianych w latach 1992-1993 w Stacji Doświadczalnej Oceny Odmian w Węgrzcach koło Krakowa, oznaczano zawartość suchej masy, azotu ogółem, azotu białkowego i aminokwasów w zależności od terminu zbioru. Zbiór ziemniaków przeprowadzono w czterech terminach: po 60, 70, 80 i 120 dniach wegetacji. Badane ziemniaki charakteryzowały się średnią zawartością azotu ogółem (1,38 % s.m.) i wysoką azotu białkowego (1,07% s.m.). Stwierdzono statystycznie istotną współzależność pomiędzy zawartością azotu ogółem i sumą aminokwasów (r=0,83), a także pomiędzy poziomem azotu białkowego i ilością aminokwasów egzogennych (r=Q.74) oraz aminokwasów endogennych (r=0,80) (tab. 1, 2, 3)

Untersuchungen über das Zuckerspektrum in Honigblaseninhalt und Honig

The sugar spectrum was investigated by HPLC in honey-sac contents of 52 fieldbees and in honey of a specially prepared colony. The origin of the honey-sac content was determined from the pollen species. The sugar spectrum has a typical frequency from the most frequent honey plants. A good correlation between the honey-sac content of the fieldbees and the honey produced in the experimental hive was found by consideration of enzyme effect (tables II and III). Liquid honeydew collected had a high sucrose content (49.42 ± 5.56%), but honeydew cristallized in the honey-sac of the stored bees (-18°C) within less than 2 wk had a lower sucrose (20.47 ± 4.78%) but a much higher melecitose content (41.12 ± 2.19% to 19.99 ± 4.10% in honeydew liquid). In this colony more than 90% of sucrose and only 30% of the melezitose collected from the bees could be inverted. The sugar spectrum of the honey-sac content provides information about the botanical origin. On the basis of the sugar spectrum of the honey the botanical origin can also be estimated

Structure of fructans in roots and leaf tissues of Lolium perenne

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