Capture of manufacturing uncertainty in turbine blades through probabilistic techniques

Efficient designing of the turbine blades is critical to the performance of an aircraft engine. An area of significant research interest is the capture of manufacturing uncertainty in the shapes of these turbine blades. The available data used for estimation of this manufacturing uncertainty inevitably contains the effects of measurement error/noise. In the present work, we propose the application of Principal Component Analysis (PCA) for de-noising the measurement data and quantifying the underlying manufacturing uncertainty. Once the PCA is performed, a method for dimensionality reduction has been proposed which utilizes prior information available on the variance of measurement error for different measurement types. Numerical studies indicate that approximately 82% of the variation in the measurements from their design values is accounted for by the manufacturing uncertainty, while the remaining 18% variation is filtered out as measurement error

Human origin recognition complex is essential for HP1 binding to chromatin and heterochromatin organization

The origin recognition complex (ORC) is a DNA replication initiator protein also known to be involved in diverse cellular functions including gene silencing, sister chromatid cohesion, telomere biology, heterochromatin localization, centromere and centrosome activity, and cytokinesis. We show that, in human cells, multiple ORC subunits associate with hetereochromatin protein 1 (HP1) alpha- and HP1beta-containing heterochromatic foci. Fluorescent bleaching studies indicate that multiple subcomplexes of ORC exist at heterochromatin, with Orc1 stably associating with heterochromatin in G1 phase, whereas other ORC subunits have transient interactions throughout the cell-division cycle. Both Orc1 and Orc3 directly bind to HP1alpha, and two domains of Orc3, a coiled-coil domain and a mod-interacting region domain, can independently bind to HP1alpha; however, both are essential for in vivo localization of Orc3 to heterochromatic foci. Direct binding of both Orc1 and Orc3 to HP1 suggests that, after the degradation of Orc1 at the G1/S boundary, Orc3 facilitates assembly of ORC/HP1 proteins to chromatin. Although depletion of Orc2 and Orc3 subunits by siRNA caused loss of HP1alpha association to heterochromatin, loss of Orc1 and Orc5 caused aberrant HP1alpha distribution only to pericentric heterochromatin-surrounding nucleoli. Depletion of HP1alpha from human cells also shows loss of Orc2 binding to heterochromatin, suggesting that ORC and HP1 proteins are mutually required for each other to bind to heterochromatin. Similar to HP1alpha-depleted cells, Orc2 and Orc3 siRNA-treated cells also show loss of compaction at satellite repeats, suggesting that ORC together with HP1 proteins may be involved in organizing higher-order chromatin structure and centromere function

A review on novel COVID-19: background, etiology, pathogenesis, transmission, prevention and management

Mankind's history is watching a bizarre time battling an imperceptible adversary, the novel COVID-19 Coronavirus. At first seen in the Wuhan province of China, presently limitlessly spreading all over world. How the COVID 19 has been made on the globe become Pandemic needs no depiction. The virus has been accounted for affecting the lungs and related respiratory tracts promoting harm of the alveoli. It has been accounted that the respiratory sickness is the prevailing Clinical symptom of COVID-19. This review article discusses an easily understanding of the causes, different type of Human viruses regarded of Coronavirus, clinical diagnosis of RT-PCR, FET, Primary prevention and control of the virus. Therefore, this review article main theme is to provide more reliable and valid information to control and manage public emergency in both acute and chronic conditions of coronaviru

Universal power law in the orientational relaxation in thermotropic liquid crystals

We observe a surprisingly general power law decay at short to intermediate times in orientational relaxation in a variety of model systems (both calamitic and discotic, and also discrete) for thermotropic liquid crystals. As all these systems transit across the isotropic-nematic phase boundary, two power law relaxation regimes, separated by a plateau, emerge giving rise to a step-like feature (well-known in glassy liquids) in the single-particle second-rank orientational time correlation function. In contrast to its probable dynamical origin in supercooled liquids, we show that the power law here can originate from the thermodynamic fluctuations of the orientational order parameter, driven by the rapid growth in the second-rank orientational correlation length.Comment: Submitted to Physical Review Letter