The Grizzly, October 27, 2005

Clamer Ghosts at it Again • Henrietta: A Haunting Tale • Spirits Spook BWC and Elliott • Terror Behind the Walls • Lighter Side of Halloween • Senior Halloween Party Promises to Bring Excitement to the Weekend • Opinions: Words that Scare People • Streak Ends as Bears Douse Bullets • Bears Blank Bulletshttps://digitalcommons.ursinus.edu/grizzlynews/1697/thumbnail.jp

The Grizzly, November 10, 2005

Congress to Campus • Spyware Protection • Honor Code Debate Continues • Human Rights Activist Speaks at UC • Scientific Sexual Healing • Epic Skate Shop • DiBlasio to Perform at Ursinus • Repeat! Dynasty Built in Second CC Crown • Fad Diets: Sometimes it is OK Not to be Trendy • Opinions: My Mouth Runneth Over; Who is Samuel Alito? • No Crown but Earn Bid to ECAC • Women\u27s Rugby Division III Championshttps://digitalcommons.ursinus.edu/grizzlynews/1699/thumbnail.jp

The Grizzly, September 15, 2005

Gas Prices Continue to Rise • Campus and Local Community Begin Relief Efforts • Students Share Study Abroad Experiences • The Deal with the Meal Deal • One of Ursinus\u27 Own Performs Professionally • Watch Out, Employers: You Could be Next! • How Much is Too Much? Your Guide to Avoiding Portion Distortion • Excitement Building in Kaleidoscope • Beyond the Condom: Guide to Safe Sex • Opinions: New Price of Driving; Ursinus, U are Worth it • Irony of Work Study • Things They Didn\u27t Teach You at Freshman Orientation • Who Says Division III Players Can\u27t Go Pro?https://digitalcommons.ursinus.edu/grizzlynews/1692/thumbnail.jp

The Grizzly, November 3, 2005

Remembering Professor McLennan: A Passionate Teacher and a Beloved Friend • Renowned Writer Speaks at Ursinus • Building Plans for Spring 2006 and Beyond • Ovarian Cancer Walk • Chikara Wrasslin\u27 • EQ vs. IQ: Hirsh-Pasek on Education • The Many Faces of Muslim Women • Take Heed and Use Your Keys • Escape Velocity\u27s Just the Start is a Great Success • New Oktoberfest Policies Put into Effect • Popping the Pill • RHA Brings Halloween Fun to Ursinus • Opinions: The Right to Write Right; Raising Rates for Resident Assistants; Global Gag Rule and FGM; Harriet Miers: Aftermath; You Snooze, You Lose • Title Hopes Still Alive as Ursinus Upsets #16 Johns Hopkins • Bears Beat Blue Jays, Look to Defend Conference Crownhttps://digitalcommons.ursinus.edu/grizzlynews/1698/thumbnail.jp

The Grizzly, September 29, 2005

Fire Safety on Campus • UC Tuition Series Part I: An Overview • Study Abroad in Madrid Returns • Campus Drive Removal • Upcoming RHA Events • Backed Up Your Computer Lately? • Club Spotlight: Le Cercle Francais • Seven Day Itch • The Drift Away Cafe • Main Street Walks for STD Awareness • Sigma Gamma Rho Walks for Sickle Cell Anemia • Update from Mexico • Heefner Organ Recital Series at Ursinus College • Readjusting: Tulane Students at Ursinus • Oktoberfest: An Ursinus Tradition • How do You Take Your Caffeine? • Opinions: Activities Fair Helps Students Get Involved; Face Off; Gangsta Mentality; This Year\u27s Fringe Festival Lived Up to its Name • Just for Kicks, Lady Bears Win Six • Bears Strand Shorewomen • Breaking the Moldhttps://digitalcommons.ursinus.edu/grizzlynews/1694/thumbnail.jp

Proteomic characterization of high-density lipoprotein particles in patients with non-alcoholic fatty liver disease

Abstract Background Metabolic diseases such as obesity and diabetes are associated with changes in high-density lipoprotein (HDL) particles, including changes in particle size and protein composition, often resulting in abnormal function. Recent studies suggested that patients with non-alcoholic fatty liver disease (NAFLD), including individuals with non-alcoholic steatohepatitis (NASH), have smaller HDL particles when compared to individuals without liver pathologies. However, no studies have investigated potential changes in HDL particle protein composition in patients with NAFLD, in addition to changes related to obesity, to explore putative functional changes of HDL which may increase the risk of cardiovascular complications. Methods From a cohort of morbidly obese females who were diagnosed with simple steatosis (SS), NASH, or normal liver histology, we selected five matched individuals from each condition for a preliminary pilot HDL proteome analysis. HDL particles were enriched using size-exclusion chromatography, and the proteome of the resulting fraction was analyzed by liquid chromatography tandem mass spectrometry. Differences in the proteomes between the three conditions (normal, SS, NASH) were assessed using label-free quantitative analysis. Gene ontology term analysis was performed to assess the potential impact of proteomic changes on specific functions of HDL particles. Results Of the 95 proteins identified, 12 proteins showed nominally significant differences between the three conditions. Gene ontology term analysis revealed that severity of the liver pathology may significantly impact the anti-thrombotic functions of HDL particles, as suggested by changes in the abundance of HDL-associated proteins such as antithrombin III and plasminogen. Conclusions The pilot data from this study suggest that changes in the HDL proteome may impact the functionality of HDL particles in NAFLD and NASH patients. These proteome changes may alter cardio-protective properties of HDL, potentially contributing to the increased cardiovascular disease risk in affected individuals. Further validation of these protein changes by orthogonal approaches is key to confirming the role of alterations in the HDL proteome in NAFLD and NASH. This will help elucidate the mechanistic effects of the altered HDL proteome on cardioprotective properties of HDL particles

36-month clinical outcomes of patients with venous thromboembolism: GARFIELD-VTE

Background: Venous thromboembolism (VTE), encompassing both deep vein thrombosis (DVT) and pulmonary embolism (PE), is a leading cause of morbidity and mortality worldwide.Methods: GARFIELD-VTE is a prospective, non-interventional observational study of real-world treatment practices. We aimed to capture the 36-month clinical outcomes of 10,679 patients with objectively confirmed VTE enrolled between May 2014 and January 2017 from 415 sites in 28 countries.Findings: A total of 6582 (61.6 %) patients had DVT alone, 4097 (38.4 %) had PE +/- DVT. At baseline, 98.1 % of patients received anticoagulation (AC) with or without other modalities of therapy. The proportion of patients on AC therapy decreased over time: 87.6 % at 3 months, 73.0 % at 6 months, 54.2 % at 12 months and 42.0 % at 36 months. At 12-months follow-up, the incidences (95 % confidence interval [CI]) of all-cause mortality, recurrent VTE and major bleeding were 6.5 (7.0-8.1), 5.4 (4.9-5.9) and 2.7 (2.4-3.0) per 100 person-years, respectively. At 36-months, these decreased to 4.4 (4.2-4.7), 3.5 (3.2-2.7) and 1.4 (1.3-1.6) per 100 person-years, respectively. Over 36-months, the rate of all-cause mortality and major bleeds were highest in patients treated with parenteral therapy (PAR) versus oral anti-coagulants (OAC) and no OAC, and the rate of recurrent VTE was highest in patients on no OAC versus those on PAR and OAC. The most frequent cause of death after 36-month follow-up was cancer (n = 565, 48.6 %), followed by cardiac (n = 94, 8.1 %), and VTE (n = 38, 3.2 %). Most recurrent VTE events were DVT alone (n = 564, 63.3 %), with the remainder PE, (n = 236, 27.3 %), or PE in combination with DVT (n = 63, 7.3 %).Interpretation: GARFIELD-VTE provides a global perspective of anticoagulation patterns and highlights the accumulation of events within the first 12 months after diagnosis. These findings may help identify treatment gaps for subsequent interventions to improve patient outcomes in this patient population

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field