DEVELOPMENT OF NEW ANALYTICAL METHOD AND ITS VALIDATION FOR THE DETERMINATION OF SALBUTAMOL SULPHATE IN BULK AND MARKETED FORMULATIONS

Objective: The present study aims to develop a simple, sensitive, specific, and validated colorimetric method for the quantitative estimation of Salbutamol Sulphate in bulk and pharmaceutical dosage form.Method: A simple colorimetric method for the determination of Salbutamol Sulphate in pure as well as in its dosage form has been developed. The method is based on formation of a blue coloured chromogen by the interaction of Salbutamol Sulphate with Folin–Ciocalteu reagent under basic condition and the maximum absorbance of the developed chromogen was found. The developed method is validated in terms of Linearity, Accuracy, Precision, Limit of Detection, Limit of Quantitation, as per ICH Guidelines.Results: The λmax was found to be 723nm for assay. Linearity was found in the concentration range of 10-50 µg/ml respectively with a correlation coefficient of 0.9979. The regression equation was found as y=bx+a: 0.0199x+0.0208.Conclusion: The proposed method makes use of simple reagent, which an ordinary analytical laboratory can afford. The method was found to be simple, precise, economic and less time consuming. The method has been statistically evaluated and results obtained are accurate, precise and insensitive and free from the interferences of other additives present in the formulation

Design, synthesis, characterization and biological evaluation of Benzothiazole-6-carboxylate derivatives

A parent benzothiazole molecule was synthesized by Jacobson synthesis, then it is subjected to treatment with various aromatic aldehydes to get the corresponding Schiff bases followed by esterification of carboxyl group by using various alcohols. The structures of synthesized compounds were confirmed by various spectroscopic methods such as IR, NMR and mass spectroscopy. The products were evaluated for their antimicrobial activity. Some of the compounds exhibited potent activity when compared with the standards

INTERNATIONAL JOURNAL OF PHARMACEUTICAL AND CHEMICAL SCIENCES Development of New Analytical Method and Its Validation for the Determination of Ethamsylate in Bulk and Marketed Formulations

ABSTRACT Method is based on the oxidation of Ethamsylate with ferric ammonium sulphate followed by complex formation of resulting ferrous ion (Fe 2+ ) with 1, 10-phenanthroline to form orange red coloured chromogen exhibiting absorption maximum at 509nm and obeyed Beer's law in the concentration range of 2-10 μg/ml. The correlation coefficient was found 0.9998. The method has been validated according to ICH Guidelines

Synthesis and anticancer evaluation of 2-phenyl thiaolidinone substituted 2-phenyl benzothiazole-6-carboxylic acid derivatives

A novel series of 2-(3-(4-oxo-2-substituted phenyl thiazolidin-3-yl)phenyl)benzo[d]thiazole-6-carboxylic acid derivatives PP1–PP8 were synthesized by various benzothiazole Schiff’s bases by reaction with thioglycollic acid. Their structures were established on the basis of IR, 1H-NMR, 13C-NMR, mass spectral data and elemental analysis. All the synthesized compounds were screened for their in vitro anticancer activity by 3-(4,5-dimethyl thiazole-2yl)-2,5-diphenyltetrazoliumbromide (MTT) assay on human cervical cancer cell line (HeLa) cell lines. Among these compound PP2 exhibited most significant activity as compared with PP5, PP7 and PP8. However, the activity was less as compared to the standard drug Cisplatin

The Marine-Derived Filamentous Fungi in Biotechnology

For a long time considered as essentially terrestrial organisms, filamentous fungi have recently disclosed to be widespread in marine habitats. Such a pervasiveness not only concerns obligate marine species but also a multitude of taxa known from disparate terrestrial substrates whose occurrence at sea, at first considered incidental, is now regarded as an evidence of extreme ecological flexibility. Actually, the peculiar physico-chemical properties of the marine environment are presumed to have induced special physiological adaptations that could be considered in view of a possible biotechnological exploitation of fungal strains recovered from marine sources. The potential of filamentous fungi reported from marine contexts for the manifold applications in biotechnology involving microbial strains is revised in this chapter