30 research outputs found

    Comprehensive tissue-specific proteome analysis of drought stress responses in Pennisetum glaucum (L.) R. Br. (Pearl millet)

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    Pearl millet is the fifth most important cereal crop worldwide and cultivated especially by small holder farmers in arid and semi-arid regions because of its drought and salt tolerance. The molecular mechanisms of drought stress tolerance in Pennisetum remain elusive. We have used a shotgun proteomics approach to investigate protein signatures from different tissues under drought and control conditions. Drought stressed plants showed significant changes in stomatal conductance and increased root growth compared to the control plants. Root, leaf and seed tissues were harvested and 2281 proteins were identified and quantified in total. Leaf tissue showed the largest number of significant changes (120), followed by roots (25) and seeds (10). Increased levels of root proteins involved in cell wall-, lipid-, secondary- and signaling metabolism and the concomitantly observed increased root length point to an impaired shoot–root communication under drought stress. The harvest index (HI) showed a significant reduction under drought stress. Proteins with a high correlation to the HI were identified using sparse partial least square (sPLS) analysis. Considering the importance of Pearl millet as a stress tolerant food crop, this study provides a first reference data set for future investigations of the underlying molecular mechanisms

    Magnesia-Based Cements: A Journey of 150 Years, and Cements for the Future?

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    This review examines the detailed chemical insights that have been generated through 150 years of work worldwide on magnesium-based inorganic cements, with a focus on both scientific and patent literature. Magnesium carbonate, phosphate, silicate-hydrate, and oxysalt (both chloride and sulfate) cements are all assessed. Many such cements are ideally suited to specialist applications in precast construction, road repair, and other fields including nuclear waste immobilization. The majority of MgO-based cements are more costly to produce than Portland cement because of the relatively high cost of reactive sources of MgO and do not have a sufficiently high internal pH to passivate mild steel reinforcing bars. This precludes MgO-based cements from providing a large-scale replacement for Portland cement in the production of steel-reinforced concretes for civil engineering applications, despite the potential for CO2 emissions reductions offered by some such systems. Nonetheless, in uses that do not require steel reinforcement, and in locations where the MgO can be sourced at a competitive price, a detailed understanding of these systems enables their specification, design, and selection as advanced engineering materials with a strongly defined chemical basis

    Changes in spectral reflectance of crop canopies due to drought stress.

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    Remote sensing at optical wavelengths provides information on agricultural crop status, therefore being a useful tool for the detection and monitoring of drought stress in crop production. In the project “crop drought stress monitoring by remote sensing” (DROSMON) led by the University of Natural Resources and Applied Life Sciences in Vienna, which started in January 2005, remote sensing methods for drought stress classification were based on physical models of canopy reflectance using a combination of SAILH and PROSPECT. Spectral reflectance of maize and wheat were measured in situ using a field spectroradiometer FieldSpec Pro FR for different crop development stages and drought stress levels at a test site in Vienna, Austria. An extensive validation program was carried out measuring various physiological properties of the crops. A significant difference in reflectance was observed between the canopies experiencing distinct drought stress levels. The observed differences could be confirmed by model simulations based on the measured biophysical variables. These suggest that there will be a change in spectral reflectance in drought stressed crops, varying according to the different growth stages. This is most marked in the near (NIR) and mid (MIR) infrared wavelength region, probably due to modifications of leaf internal structure, variations in leaf inclination (e.g. due to wilting) and leaf area index. We present initial results from this research, which partly support these ideas. Further investigations are necessary
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