Towards an integrated understanding of gut microbiota using insects as model systems

© 2014 Published by Elsevier Ltd. Metazoans form symbioses with microorganisms that synthesize essential nutritional compounds and increase their efficiency to digest and absorb nutrients. Despite the growing awareness that microbes within the gut play key roles in metabolism, health and development of metazoans, symbiotic relationships within the gut are far from fully understood. Insects, which generally harbor a lower microbial diversity than vertebrates, have recently emerged as potential model systems to study these interactions. In this review, we give a brief overview of the characteristics of the gut microbiota in insects in terms of low diversity but high variability at intra- and interspecific levels and we investigate some of the ecological and methodological factors that might explain such variability. We then emphasize how studies integrating an array of techniques and disciplines have the potential to provide new understanding of the biology of this micro eco-system

Tissue-specific immune gene expression in the migratory locust, Locusta Migratoria

© 2015 by the authors; licensee MDPI, Basel, Switzerland. The ability of hosts to respond to infection involves several complex immune recognition pathways. Broadly conserved pathogen-associated molecular patterns (PAMPs) allow individuals to target a range of invading microbes. Recently, studies on insect innate immunity have found evidence that a single pathogen can activate different immune pathways across species. In this study, expression changes in immune genes encoding peptidoglycan-recognition protein SA (PGRP-SA), gram-negative binding protein 1 (GNBP1) and prophenoloxidase (ProPO) were investigated in Locusta migratoria, following an immune challenge using injected lipopolysaccharide (LPS) solution from Escherichia coli. Since immune activation might also be tissue-specific, gene expression levels were followed across a range of tissue types. For PGRP-SA, expression increased in response to LPS within all seven of the tissue-types assayed and differed significantly between tissues. Expression of GNBP1 similarly varied across tissue types, yet showed no clear expression difference between LPS-injected and uninfected locusts. Increases in ProPO expression in response to LPS, however, could only be detected in the gut sections. This study has revealed tissue-specific immune response to add a new level of complexity to insect immune studies. In addition to variation in recognition pathways identified in previous works, tissue-specificity should be carefully considered in similar works

Spacetime structure of the global vortex

We analyse the spacetime structure of the global vortex and its maximal analytic extension in an arbitrary number of spacetime dimensions. We find that the vortex compactifies space on the scale of the Hubble expansion of its worldvolume, in a manner reminiscent of that of the domain wall. We calculate the effective volume of this compactification and remark on its relevance to hierarchy resolution with extra dimensions. We also consider strongly gravitating vortices and derive bounds on the existence of a global vortex solution.Comment: 19 pages revtex, 2 figures, minor changes, references adde

Radiative corrections to the lightest KK states in the T^2/(Z_2\times Z_2') orbifold

We study radiative corrections localized in the fixed points of the orbifold for the field theory in six dimensions with two dimensions compactified on the $T_2/(Z_2\times Z_2')$ orbifold in a specific realistic model for low energy physics that solves the proton decay and neutrino mass problem. We calculate corrections to the masses of the lightest stable KK modes, which could be the candidates for the dark matter.Comment: 14 pages, 2 figure

Material-independent crack arrest statistics: Application to indentation experiments

An extensive experimental study of indentation and crack arrest statistics is presented for four different brittle materials (alumina, silicon carbide, silicon nitride, glass). Evidence is given that the crack length statistics can be described by a universal (i.e. material independent) distribution. The latter directly derives from results obtained when modeling crack propagation as a depinning phenomenon. Crack arrest (or effective toughness) statistics appears to be fully characterized by two parameters, namely, an asymptotic crack length (or macroscopic toughness) value and a power law size dependent width. The experimental knowledge of the crack arrest statistics at one given scale thus gives access to its knowledge at all scales

Effective description of brane terms in extra dimensions

We study how theories defined in (extra-dimensional) spaces with localized defects can be described perturbatively by effective field theories in which the width of the defects vanishes. These effective theories must incorporate a ``classical'' renormalization, and we propose a renormalization prescription a la dimensional regularization for codimension 1, which can be easily used in phenomenological applications. As a check of the validity of this setting, we compare some general predictions of the renormalized effective theory with those obtained in a particular ultraviolet completion based on deconstruction.Comment: 28 page

Scalar Hair of Global Defect and Black Brane World

We consider a complex scalar field in (p+3)-dimensional bulk with a negative cosmological constant and study global vortices in two extra-dimensions. We reexamine carefully the coupled scalar and Einstein equations, and show that the boundary value of scalar amplitude at infinity of the extra-dimensions should be smaller than vacuum expectation value. The brane world has a cigar-like geometry with an exponentially decaying warp factor and a flat thick p-brane is embedded. Since a coordinate transformation identifies the obtained brane world as a black p-brane world bounded by a horizon, this strange boundary condition of the scalar amplitude is understood as existence of a short scalar hair.Comment: 26 pages, 2 figure

Finite temperature Casimir effect for massive scalar field in spacetime with extra dimensions

We compute the finite temperature Casimir energy for massive scalar field with general curvature coupling subject to Dirichlet or Neumann boundary conditions on the walls of a closed cylinder with arbitrary cross section, located in a background spacetime of the form $M^{d_1+1}\times \mathcal{N}^n$, where $M^{d_1+1}$ is the $(d_1+1)$-dimensional Minkowski spacetime and $\mathcal{N}^n$ is an $n$-dimensional internal manifold. The Casimir energy is regularized using the criteria that it should vanish in the infinite mass limit. The Casimir force acting on a piston moving freely inside the closed cylinder is derived and it is shown that it is independent of the regularization procedure. By letting one of the chambers of the cylinder divided by the piston to be infinitely long, we obtain the Casimir force acting on two parallel plates embedded in the cylinder. It is shown that if both the plates assume Dirichlet or Neumann boundary conditions, the strength of the Casimir force is reduced by the increase in mass. Under certain conditions, the passage from massless to massive will change the nature of the force from long range to short range. Other properties of the Casimir force such as its sign, its behavior at low and high temperature, and its behavior at small and large plate separations, are found to be similar to the massless case. Explicit exact formulas and asymptotic behaviors of the Casimir force at different limits are derived. The Casimir force when one plate assumes Dirichlet boundary condition and one plate assumes Neumann boundary condition is also derived and shown to be repulsive.Comment: 28 pages, 4 figure

ACQUIRING SEISMIC DATA IN THE NORTH-EAST OF ITALY: THE OGS-CRS EXPERIENCE IN USING THE ANTELOPE SOFTWARE SUITE

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Mediation of supersymmetry breaking in extra dimensions

We review the mechanisms of supersymmetry breaking mediation that occur in sequestered models, where the visible and the hidden sectors are separated by an extra dimension and communicate only via gravitational interactions. By locality, soft breaking terms are forbidden at the classical level and reliably computable within an effective field theory approach at the quantum level. We present a self-contained discussion of these radiative gravitational effects and the resulting pattern of soft masses, and give an overview of realistic model building based on this set-up. We consider both flat and warped extra dimensions, as well as the possibility that there be localized kinetic terms for the gravitational fields.Comment: LaTex, 15 pages; brief review prepared for MPLA. v2: minor correction