Quantifying spatial, temporal, angular and spectral structure of effective daylight in perceptually meaningful ways

We present a method to capture the 7-dimensional light field structure, and translate it into perceptually-relevant information. Our spectral cubic illumination method quantifies objective correlates of perceptually relevant diffuse and directed light components, including their variations over time, space, in color and direction, and the environment's response to sky and sunlight. We applied it 'in the wild', capturing how light on a sunny day differs between light and shadow, and how light varies over sunny and cloudy days. We discuss the added value of our method for capturing nuanced lighting effects on scene and object appearance, such as chromatic gradients

Verdrängung widerständiger Lesarten? : Medien und Macht bei Jean Baudrillard und Stuart Hall

Die Arbeit behandelt einen Vergleich der Medientheorien Jean Baudrillards und Stuart Halls. Im Fokus steht die Frage nach der ‚Macht der Medien’: Versteht man Massenmedien als wichtigen Integrationsfaktor pluralistischer Gesellschaften – sie geben einen Orientierungsrahmen ‚relevanten’ Wissens an –, so sind sie gleichzeitig auch Machtfaktoren. Als radikal-apokalyptischer Theoretiker einer von medialen ‚Simulationen’ durchdrungenen Welt, sah Jean Baudrillard durch Massenmedien eine allumfassende gesellschaftliche Kontrolle eingerichtet. Aus Sicht der Cultural Studies, für deren Beschäftigung mit den Medien Stuart Hall wegweisend war und ist, treffen Medieninhalte auf ein Publikum, das immer schon über Erfahrungen, Wissen und Prägungen verfügt und daraus seine Präferenzen im Mediengebrauch ableitet – und auch entsprechende Kompetenzen ausbildet. Die Verbindung von Medien und Macht geschieht anhand des von Michel Foucault aufgestellten Modells des ‚Dipositivs’, das von Johanna Dorer für die Analyse öffentlicher Kommunikation fruchtbar gemacht worden ist. Der Blick wird hier auch auf das Internet gerichtet, durch dessen Verbreitung sich die Kommunikationslandschaft grundlegend gewandelt hat

Perceptual Qualities of Optically Mixed Materials

We present a novel setup in which real objects made of two different materials can be mixed optically in a linearly weighted manner. We conducted a psychophysical experiment in which observers rated optical mixtures of the three combinations of glossy, matte, and velvety green birds. The observers rated the materials on four scales: matte–glossy, hard–soft, cold–warm, and light-heavy. The judgments were found to be consistent and varied systematically with the weights of the contributions. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down

Uncovering the Signaling Landscape Controlling Breast Cancer Cell Migration Identifies Novel Metastasis Driver Genes

Ttriple-negative breast cancer (TNBC) is an aggressive and highly metastatic breast cancer subtype. Enhanced TNBC cell motility is a prerequisite of TNBC cell dissemination. Here, we apply an imaging-based RNAi phenotypic cell migration screen using two highly motile TNBC cell lines (Hs578T and MDA-MB-231) to provide a repository of signaling determinants that functionally drive TNBC cell motility. We have screened ~4,200 target genes individually and discovered 133 and 113 migratory modulators of Hs578T and MDA-MB-231, respectively, which are linked to signaling networks predictive for breast cancer progression. The splicing factors PRPF4B and BUD31 and the transcription factor BPTF are essential for cancer cell migration, amplified in human primary breast tumors and associated with metastasis-free survival. Depletion of PRPF4B, BUD31 and BPTF causes primarily down regulation of genes involved in focal adhesion and ECM-interaction pathways. PRPF4B is essential for TNBC metastasis formation in vivo, making PRPF4B a candidate for further drug developmen

An improved model to study tumor cell autonomous metastasis programs using MTLn3 cells and the Rag2−/− γc−/− mouse

The occurrence of metastases is a critical determinant of the prognosis for breast cancer patients. Effective treatment of breast cancer metastases is hampered by a poor understanding of the mechanisms involved in the formation of these secondary tumor deposits. To study the processes of metastasis, valid in vivo tumor metastasis models are required. Here, we show that increased expression of the EGF receptor in the MTLn3 rat mammary tumor cell-line is essential for efficient lung metastasis formation in the Rag mouse model. EGFR expression resulted in delayed orthotopic tumor growth but at the same time strongly enhanced intravasation and lung metastasis. Previously, we demonstrated the critical role of NK cells in a lung metastasis model using MTLn3 cells in syngenic F344 rats. However, this model is incompatible with human EGFR. Using the highly metastatic EGFR-overexpressing MTLn3 cell-line, we report that only Rag2−/−γc−/− mice, which lack NK cells, allow efficient lung metastasis from primary tumors in the mammary gland. In contrast, in nude and SCID mice, the remaining innate immune cells reduce MTLn3 lung metastasis formation. Furthermore, we confirm this finding with the orthotopic transplantation of the 4T1 mouse mammary tumor cell-line. Thus, we have established an improved in vivo model using a Rag2−/− γc−/− mouse strain together with MTLn3 cells that have increased levels of the EGF receptor, which enables us to study EGFR-dependent tumor cell autonomous mechanisms underlying lung metastasis formation. This improved model can be used for drug target validation and development of new therapeutic strategies against breast cancer metastasis formation

Uncovering the signaling landscape controlling breast cancer cell migration identifies novel metastasis driver genes

Ttriple-negative breast cancer (TNBC) is an aggressive and highly metastatic breast cancer subtype. Enhanced TNBC cell motility is a prerequisite of TNBC cell dissemination. Here, we apply an imaging-based RNAi phenotypic cell migration screen using two highly motile TNBC cell lines (Hs578T and MDA-MB-231) to provide a repository of signaling determinants that functionally drive TNBC cell motility. We have screened ~4,200 target genes individually and discovered 133 and 113 migratory modulators of Hs578T and MDA-MB-231, respectively, which are linked to signaling networks predictive for breast cancer progression. The splicing factors PRPF4B and BUD31 and the transcription factor BPTF are essential for cancer cell migration, amplified in human primary breast tumors and associated with metastasis-free survival. Depletion of PRPF4B, BUD31 and BPTF causes primarily down regulation of genes involved in focal adhesion and ECM-interaction pathways. PRPF4B is essential for TNBC metastasis formation in vivo, making PRPF4B a candidate for further drug development