39 research outputs found
Overview of the Role for Calreticulin in the Enhancement of Wound Healing through Multiple Biological Effects
Calreticulin (CRT), an intracellular chaperone protein crucial for the proper folding and transport of proteins through the endoplasmic reticulum, has more recent acclaim as a critical regulator of extracellular functions, particularly in mediating cellular migration and as a requirement for phagocytosis of apoptotic cells. Consistent with these functions, we show that the topical application of CRT has profound effects on the process of wound healing by causing a dose-dependent increase in epithelial migration and granulation tissue formation in both murine and porcine normal and impaired animal models of skin injury. These effects of CRT are substantiated, in vitro, as we show that CRT strongly induces cell migration/wound closure of human keratinocytes and fibroblasts, using a wound/scratch plate assay, and stimulates cellular proliferation of human keratinocytes, fibroblasts, and vascular endothelial cells, providing mechanistic insight into how CRT functions in repair. Similarly, in both animal models, the histology of the wounds show marked proliferation of basal keratinocytes and dermal fibroblasts, dense cellularity of the dermis with notably increased numbers of macrophages and well-organized collagen fibril deposition. Thus, CRT profoundly affects the wound healing process by recruiting cells essential for repair into the wound, stimulating cell growth, and increasing extracellular matrix production
Railway Systems and the 'Universal Good of the State': Technologies of Government in the 19th-Century Papal State
Informed by Foucaultās concept of governmentality, the paper focuses on nineteenth-century General Commissariat for the Railroad Industry in the Papal State. Unlike in liberal States, where government intervention in the affairs of railway companies was limited, the pressing need to reinforce the Popeās pastoral power, strengthen the bond between the believers and the Holy See and ensure equity and the efficiency of the new infrastructure meant that the Commissariat acted as a governmental centre of calculation. Accounting technologies in the form of budgets, cost accounting systems and penetrating audits enabled the government to intervene in the operations of private railway companies. The study analyses the role of accounting and auditing practices in the pursuit of non-liberal goals in an industry which is traditionally perceived as critical to the development of a liberal economy, one in which accounting was traditionally used to maintain investorsā confidence in the capitalist system
State-owned MNCs and host country expropriation risk: The role of home state soft power and economic gunboat diplomacy
Imaging Mass Spectrometry for Assessing Cutaneous Wound Healing: Analysis of Pressure Ulcers
Imaging
mass spectrometry (IMS) was employed for the analysis of
frozen skin biopsies to investigate the differences between stage
IV pressure ulcers that remain stalled, stagnant, and unhealed versus
those exhibiting clinical and histological signs of improvement. Our
data reveal a rich diversity of proteins that are dynamically modulated,
and we selectively highlight a family of calcium binding proteins
(S-100 molecules) including calcyclin (S100-A6), calgranulins A (S100-A8)
and B (S100-A9), and calgizzarin (S100-A11). IMS allowed us to target
three discrete regions of interest: the wound bed, adjacent dermis,
and hypertrophic epidermis. Plots derived using unsupervised principal
component analysis of the global protein signatures within these three
spatial niches indicate that these data from wound signatures have
potential as a prognostic tool since they appear to delineate wounds
that are favorably responding to therapeutic interventions versus
those that remain stagnant or intractable in their healing status.
Our discovery-based approach with IMS augments current knowledge of
the molecular signatures within pressure ulcers while providing a
rationale for a focused examination of the role of calcium modulators
within the context of impaired wound healing